Quercetin prevents insulin dysfunction in hypertensive animals

Serra, Cristiane Alves; Reis, Alexandre Freire dos; Calsa, Bruno; Bueno, Cintia Sena; Helaehil, Júlia Venturini; Souza, Suelen Aparecida Ribeiro de; Oliveira, Camila Andréa de; Vanzella, Emerielle Cristine; Amaral, Maria Esméria Corezola do

doi:10.1007/s40200-022-00987-4

Cited by 8 publications

(3 citation statements)

References 58 publications

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“…Serum and liver TBARS contents were determined as described previously (Serra et al 2022). Briefly, 50 μL of serum or liver homogenate was mixed with 10 μL of 0.1 M Butylated Hydroxytoluene, 400 μL of 1% 2-thiobarbituric acid (TBA; Merck, St. Charles, MO, USA), and 200 μL of 20% phosphoric acid and heated at 100 °C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized mice

Sapatini

Calsa

Marin

et al. 2023

Preprint

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Loss of ovarian function is associated with increased visceral fat. In this study, we aimed to study the effects of caloric restriction (CR) on metabolism in ovariectomized mice. Female, 8 to 12 month old mice were divided into three groups: OVX (ovariectomized mice), OVXR (ovariectomized mice on forty percent CR) and Sham. CR increased insulin sensitivity and glucose tolerance as evidenced by the results of intraperitoneal glucose tolerance and intraperitoneal insulin tolerance tests, liver glycogen levels, and homeostasis model assessment indexes. Increased AMPK phosphorylation was observed in the liver of OVXR mice. CR also increased hepatic cholesterol and triglyceride levels. The reductions in the level of TBARS in the serum and liver and of hydrogen peroxide in the liver of OVXR mice suggested alterations in the redox state of the liver. Although expression of catalase protein was reduced by CR, expression of superoxide dismutase was not altered by CR. Although interleukin IL6 and IL10 levels in OVXR mice were similar to those in Sham mice, macrophage infiltration was reduced in OVXR mice as evidenced by n-acetylglucosaminidase activity. OVXR mice had increased sirtuin (SIRT)1 levels and decreased SIRT3 levels in the liver. In conclusion, CR improved the condition of ovariectomized mice by reducing adiposity and increasing insulin sensitivity and glucose tolerance through a mechanism that may involve AMPK.

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Section: Methodsmentioning

confidence: 99%

Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized mice

Sapatini

Calsa

Marin

et al. 2023

Preprint

Self Cite

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show abstract

“…Similar to hesperetin, quercetin extracted from T. alexandrinum exhibited beneficial anti-diabetic effects in vitro and in vivo study [ 209 ]. Another study investigated the antioxidant effects of quercetin on insulin production, signaling, and action in hypertensive rats, showing reduced serum lipid peroxidation rates, improved insulin sensitivity, and increased expression of CAT, VEGF, and M3R [ 286 ].…”

Section: In Vivo and In Vitro Anti-diabet...mentioning

confidence: 99%

Clinical applications and mechanism insights of natural flavonoids against type 2 diabetes mellitus

Bouyahya,

Balahbib,

Khalid

et al. 2024

Heliyon

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“…Serum and liver TBARS contents were determined as described previously [32]. Brie y, 50 µL of serum or liver homogenate was mixed with 10 µL of 0.1 M Butylated Hydroxytoluene, 400 µL of 1% 2-thiobarbituric acid (TBA; Merck, St. Charles, MO, USA), and 200 µL of 20% phosphoric acid and heated at 100°C for 15 min.…”

Section: Hepatic and Systemic Tbarsmentioning

confidence: 99%

Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized mice

et al. 2023

Self Cite

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BackgroundLoss of ovarian function is associated with increased visceral fat. In this study, we aimed to study the effects of caloric restriction (CR) on metabolism in ovariectomized mice. Methods and ResultsFemale, 8-12-month-old mice were divided into three groups: OVX (ovariectomized mice), OVXR (40% CR) and Sham. CR increased insulin sensitivity and glucose tolerance. AMPK phosphorylation was observed in the liver of OVXR mice. CR also increased hepatic cholesterol and triglyceride levels. The reductions in the level of TBARS in the serum and liver and of H2O2 in the liver of OVXR mice suggested alterations in the redox state of the liver. Although expression of catalase protein was reduced by CR, expression of superoxide dismutase was not altered by CR. Although interleukin IL-6 and IL-10 levels in OVXR mice were similar to those in Sham mice, macrophage in ltration was reduced in OVXR mice.OVXR mice had increased sirtuin1 levels and decreased sirtuin3 levels in the liver. ConclusionsIn conclusion, CR improved the condition of ovariectomized mice by reducing adiposity and increasing insulin sensitivity and glucose tolerance through a mechanism that may involve AMPK.

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Quercetin prevents insulin dysfunction in hypertensive animals

Cited by 8 publications

References 58 publications

Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized mice

Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized mice

Clinical applications and mechanism insights of natural flavonoids against type 2 diabetes mellitus

Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized mice

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