Quercetin protects oral mucosal keratinocytes against lipopolysaccharide-induced inflammatory toxicity by suppressing the AKT/AMPK/mTOR pathway

Cao, Junhua; Xue, Rui; He, Biao

doi:10.1080/08923973.2021.1948565

Cited by 9 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, quercetin seems to reduce the IL-1β, IL-6, IL-8 and TNF-α production and the COX-2 activity, suppressing the AKT/AMPK/mTOR pathway, in oral mucosal keratinocytes. These beneficial actions demonstrate quercetin's protective role in countering inflammatory oral diseases [95].…”

Section: Anti-inflammatory Effectsmentioning

confidence: 90%

Natural Bioactive Compounds in the Management of Oral Diseases in Nephropathic Patients

Basilicata

Lauro

Campolattano

et al. 2022

IJERPH

View full text Add to dashboard Cite

Among the chronic non-communicable degenerative diseases (CDNCDs), chronic kidney disease (CKD) represents a global public health problem. Recent studies demonstrate a mutual cause–effect relationship between CKD and oral diseases, in which the presence of one induces the onset and faster progression of the other. In particular, the oral cavity alterations more frequent in CKD patients are: chronic periodontitis diseases, bone lesions, oral infections, and oral cancer lesions. Currently, a standardized therapy for the treatment of oral diseases is lacking. For this reason, natural bioactive compounds (NBCs), characterized by several health effects, such as antioxidant, antimicrobial, anti-inflammatory and anti-cancer actions, represent a new possible adjuvant therapy in the management of these pathological conditions. Among NBCs, polyphenols play a leading role due to positive modulation of oral microbiota, preventing and correcting oral dysbiosis. Moreover, these compounds exert anti-inflammatory effects, such as inhibiting the production of pro-inflammatory cytokines and the expression of cycloxigenase-2. In this light, the formulation of a new mouthwash/gel/gingival paste, with a high content of polyphenols in association with NBCs characterized by antimicrobial action, could represent a future therapy of oral disease in CKD patients.

show abstract

Section: Anti-inflammatory Effectsmentioning

confidence: 90%

Natural Bioactive Compounds in the Management of Oral Diseases in Nephropathic Patients

Basilicata

Lauro

Campolattano

et al. 2022

IJERPH

View full text Add to dashboard Cite

show abstract

“…Also, our study showed an increased expression of AMPK and a decreased activation of mTOR after LPS treatment. There are a number of studies supporting our observation of LPS-induced alterations in AMPK/mTOR signaling various cellular systems [ 50 , 51 , 52 , 53 ]. We also observed a moderate increase in AMPK activation and a moderate decrease in mTOR phosphorylation after AZD treatment.…”

Section: Discussionmentioning

confidence: 59%

Azadirachtin Attenuates Lipopolysaccharide-Induced ROS Production, DNA Damage, and Apoptosis by Regulating JNK/Akt and AMPK/mTOR-Dependent Pathways in Rin-5F Pancreatic Beta Cells

John

Raza

2021

Biomedicines

View full text Add to dashboard Cite

Pancreatic inflammation and the resulting cellular responses have been implicated in pancreatitis, diabetes, and pancreatic cancer. Inflammatory responses due to the bacterial endotoxin, lipopolysaccharide (LPS), have been demonstrated to alter cellular metabolism, autophagy, apoptosis, and cell proliferation in different cell populations, and hence increases the risks for organ toxicity including cancer. The exact molecular mechanism is however not clear. In the present study, we investigated the role and mechanism of an antioxidant, azadirachtin (AZD), a limonoid extracted from the neem tree (Azadirachta indica), against LPS-induced oxidative stress in the pancreatic β-cell line, Rin-5F. We demonstrated that cells treated with LPS (1 µg/mL for 24 h) showed increased reactive oxygen species (ROS) production, DNA damage, cell cycle arrest, and apoptosis. Our results also showed that LPS induced alterations in the AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathways, suppressing autophagy and augmenting apoptosis. Treatment with Azadirachtin (25 µM for 24 h), on the other hand, rendered some degree of protection to the pancreatic cells from apoptosis by inducing the autophagy signals required for cell survival. These results may have significance in elucidating the mechanisms of pancreatic β-cell survival and death by balancing the molecular communication between autophagy and apoptosis under inflammatory and pathological conditions.

show abstract

“…Over the years, numerous studies have reported the use of curcumin and quercetin as natural anti-apoptotic agents in tissue healing and regeneration [ 48 , 49 , 50 ]. The anti-apoptotic effect of quercetin was reported previously in LPS-stimulated oral mucosal keratinocytes by significantly decreasing the expression of Bax and increasing the expression of Bcl-2 in a dose-dependent manner [ 51 ]. Curcumin was also shown to protect keratinocytes from apoptosis and mitochondrial damage by inhibiting Caspase-3 and Caspase-9 activation, in addition to increasing ERK phosphorylation [ 52 ].…”

Section: Discussionmentioning

confidence: 92%

Development and Investigation of an Innovative 3D Biohybrid Based on Collagen and Silk Sericin Enriched with Flavonoids for Potential Wound Healing Applications

Sleiman,

Lazăr (Popa),

Albu-Kaya

et al. 2024

Polymers

View full text Add to dashboard Cite

Skin tissue injuries necessitate particular care due to associated complex healing mechanisms. Current investigations in the domain of tissue engineering and regenerative medicine are focused on obtaining novel scaffolds adapted as potential delivery systems to restore lost tissue functions and properties. In this study, we describe the fabrication and evaluation of a novel 3D scaffold structure based on collagen and silk sericin (CollSS) enriched with microcapsules containing natural compounds, curcumin (C), and/or quercetin (Q). These 3D composites were characterized by FT-IR spectroscopy, water uptake, in vitro collagenase degradation, and SEM microscopy. Furthermore, they were biologically evaluated in terms of biocompatibility, cell adhesion, anti-inflammatory, and antioxidant properties. All tested materials indicated an overall suitable biocompatibility, with the best results obtained for the one containing both flavonoids. This study suggests the cumulative beneficial effect of C and Q, encapsulated in the same composite, as a potential non-invasive therapeutic strategy for skin tissue regeneration in patients suffering from chronic wounds.

show abstract

Quercetin protects oral mucosal keratinocytes against lipopolysaccharide-induced inflammatory toxicity by suppressing the AKT/AMPK/mTOR pathway

Cited by 9 publications

References 31 publications

Natural Bioactive Compounds in the Management of Oral Diseases in Nephropathic Patients

Natural Bioactive Compounds in the Management of Oral Diseases in Nephropathic Patients

Azadirachtin Attenuates Lipopolysaccharide-Induced ROS Production, DNA Damage, and Apoptosis by Regulating JNK/Akt and AMPK/mTOR-Dependent Pathways in Rin-5F Pancreatic Beta Cells

Development and Investigation of an Innovative 3D Biohybrid Based on Collagen and Silk Sericin Enriched with Flavonoids for Potential Wound Healing Applications

Contact Info

Product

Resources

About