Quercetin Reduces Inflammation and Protects Gut Microbiota in Broilers

Abstract

The aim of this study was to investigate the effects of quercetin on inflammatory response and intestinal microflora in broiler chicken jejuna. A total of 120 broiler chickens were allocated into 3 groups: saline-challenged broilers fed a basal diet (CTR group), lipopolysaccharide (LPS)-challenged broilers fed a basal diet (L group) and LPS-challenged broilers fed a basal diet supplemented with 200 mg/kg quercetin (LQ group). Our results showed that LPS significantly increased expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-8, interferon (IFN)-γ, toll-like receptor (TLR)-4, Bax, Caspase-3 and diamine oxidase activity (DAO), and decreased expression of zona occludens-1 (ZO-1), Occludin and Bcl-2 in the jejunum, while dietary quercetin prevented the adverse effects of LPS injection. LPS injection significantly decreased the number of Actinobacteria, Armatimonadetes and Fibrobacteriae at the phylum level when compared to the CTR group. Additionally, at genus level, compared with the CTR group, the abundance of Halomonas, Micromonospora, Nitriliruptor, Peptococcus, Rubellimicrobium, Rubrobacter and Slaclda in L group was significantly decreased, while dietary quercetin restored the numbers of these bacteria. In conclusion, our results demonstrated that dietary quercetin could alleviate inflammatory responses of broiler chickens accompanied by modulating jejunum microflora.

Causal associations between gut microbiota and adverse pregnancy outcomes: A two-sample Mendelian randomization study

“…Butyricimonas, Marvinbryantia, and Oscillibacter can produce butyrate, which participates in regulating gut permeability and induces the inflammatory response (Jang et al, 2020;Chen B. D. et al, 2021;Chen Z. et al, 2021;Liu et al, 2022). Prior studies have reported that the abundance of Peptococcus increases when intestinal inflammation occurs, indicating its proinflammatory effects (Sun et al, 2022). The exact mechanism of Peptococcus on the pathogenesis of PPH warrants verification studies.…”

Effects of dietary supplementation with quercetagetin on nutrient digestibility, intestinal morphology, immunity, and antioxidant capacity of broilers

“…Flavonols have numerous biological functions. For instance, quercetin enhances the antioxidant capacity of broilers' intestines, alleviates intestinal inflammatory reactions, regulates the internal intestinal environment, maintains the integrity of the intestinal barrier, and improves the structure and morphology of the intestinal tract (15)(16)(17). Rutin exhibits antioxidant and anti-inflammatory effects by inhibiting the production of reactive oxygen species and cell apoptosis (18).…”

Targeting Oxidative Stress and Inflammation in Intervertebral Disc Degeneration: Therapeutic Perspectives of Phytochemicals

“…Quercetin is a type of natural flavonoid isolated from various fruits and vegetables ( Mirazimi et al, 2022 ). Previous studies have shown that quercetin possesses many functions, including anti-cancer, anti-oxidative, and anti-inflammatory properties ( Pinheiro et al, 2021 ; Yin et al, 2021 ; Sun et al, 2022 ). Wang et al found that quercetin can activate the autophagy pathway through SIRT1 so as to reduce the level of ROS and alleviate apoptosis and extracellular matrix degeneration ( Wang D. et al, 2020 ).…”