Quercetin stimulation of calcium release from rabbit skeletal muscle sarcoplasmic reticulum

Watras, J; Glezen, Sharon; Seifert, Christina; Katz, Arnold M.

doi:10.1016/0024-3205(83)90033-4

Cited by 16 publications

(5 citation statements)

References 39 publications

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“…The dependence of the effect of quercetin on Ca 2+ concentration is somewhat different from those of Watras et al (1982), who reported that the Ca release by quercetin was stimulated only at between 0.1 and 1 ~,M Ca 2+. Our results are rather similar to those by F, dm et al (1983;Fig.…”

Section: Dependence On Ca 2+ Concentration On Effect Of Quercetinmentioning

confidence: 51%

“…Quercetin is now known to have a dual action on sarcoplasmic reticulum vesicles: Ca releasing action, and inhibition of Ca uptake and Ca2+-Mg 2+ ATPase activity (Shoshan et al, 1980;Kirino & Shimizu, 1982;Watras et al, 1982;Kim et al, 1983;Palade et aI., 1983;Kurebayashi & Ogawa, 1984). We have also demonstrated a caffeine-like Ca releasing action of quercetin on sarcoplasmic reticulum in skinned fibres (Kurebayashi & Ogawa, 1983, 1985b.…”

Section: Introductionmentioning

confidence: 65%

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Characterization of increased Ca2+ efflux by quercetin from the sarcoplasmic reticulum in frog skinned skeletal muscle fibres

Kurebayashi

Ogawa

1986

J Muscle Res Cell Motil

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To clarify further the characteristics of the Ca-releasing action of quercetin, we examined the effect of quercetin on Ca2+ efflux from the sarcoplasmic reticulum without Ca uptake activity by omission of ATP in the presence of a high concentration (10 mM EGTA) of Ca2+ buffer, using mechanically skinned fibres from frog skeletal muscles. Quercetin increases the rate of Ca release in the presence of Ca2+ and shifts the relationship between Ca2+ concentration and the rate of Ca release to a lower range of Ca2+ concentrations. AMP potentiates the effect of quercetin. Mg2+ and procaine decrease the rate of Ca release in the presence of quercetin. These findings indicate that quercetin is very similar to caffeine; in fact, experiments confirmed that quercetin shares the site(s) of action with caffeine. Subsequent findings, however, suggest that the mechanism of the drug-induced Ca release is not as simple as expected with caffeine which may increase the affinity for Ca2+ of the 'Ca2+-induced Ca-release' mechanism, and that drugs, Ca2+ and nucleotide interact with one another in a more complex way to open the gate for Ca release. In the presence of 10 mM EGTA quercetin also causes Ca release in the virtual absence of Ca2+ if a sufficient amount of AMP is present, and the rate of Ca release in the presence of 1.55 micron Ca2+ showed different dependence on AMP concentration with and without quercetin. A higher concentration of Mg2+ is required in the presence of AMP than in the absence of AMP in order to eliminate Ca release by quercetin.

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Section: Dependence On Ca 2+ Concentration On Effect Of Quercetinmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 65%

Characterization of increased Ca2+ efflux by quercetin from the sarcoplasmic reticulum in frog skinned skeletal muscle fibres

Kurebayashi

Ogawa

1986

J Muscle Res Cell Motil

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“…Thus, quercetin must increase unidirectional efflux, and in particular potentiate its stimulation by choline Cl . Recent studies on SR vesicles also suggest incomplete influx inhibition and direct effects on efflux (Kurino and Shimizu, 1982 ;Kim et al ., 1983 ;Watras et al ., 1983 ;Kurebayashi and Ogawa, 1985). The evidence for direct efflux potentiation makes it necessary to re-evaluate inferences based on the assumption that quercetin potentiates net release only by influx inhibition, for example that pump carriers uncoupled from ATPase activity could not function in Ca release (Shoshan et al ., 1980).…”

Section: Effect Ofquercetin On "Ca Movementmentioning

confidence: 99%

Excitation of skinned muscle fibers by imposed ion gradients. II. Influence of quercetin and ATP removal on the Ca2+-insensitive component of stimulated 45Ca efflux.

Stephenson¹

1985

The Journal of General Physiology

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Ionic gradients imposed by choline Cl replacement of K methanesulfonate (Mes) at constant [K][Cl] product stimulate "Ca efflux from skinned muscle fibers ; a small, sustained Ca"-insensitive efflux component, observed in EGTA, appears to grade a much larger Ca"-dependent component responsible for contractile activation and is likely to reflect intermediate steps in excitation-contraction coupling. The present studies examined ATP-related effects on the Ca"-insensitive stimulation . "Ca efflux was measured on segments of frog semitendinosus muscle skinned by microdissection, with isometric force monitored continuously . The Ca"-insensitive component was potentiated by quercetin, a flavonoid thought to inhibit the sarcoplasmic reticulum (SR) Ca pump by stabilizing a phosphorylated intermediate . Quercetin increased the stimulated net "Ca release in the absence of EGTA, as expected from inhibition of reaccumulation, but its effectiveness in EGTA indicated potentiation of unidirectional efflux as such . Quercetin also increased unstimulated (control) "Ca efflux in EGTA, to a smaller extent ; potentiation appeared to be a function of efflux, with stimulation above control loss increased -2 .6-fold . ATP removal before stimulation, which led to rigor force and increased stiffness, prevented all quercetin effects in EGTA . ATP removal by itselfinhibited ionic stimulation of the Ca"-insensitive component, with little residual increase above the parallel control loss. Addition of the nonhydrolyzable ATP analogue AMP-PCP ([adenylyl-/3,-y-methylene]diphosphate) (0 .8 mM) after ATP removal gave similar results to ATP-free solution, which suggests that adenine nucleotide binding alone does not support stimulation by choline Cl . These results imply a fundamental role for ATP in the excitation of skinned fibers by imposed diffusion potentials; they also suggest that ATP regulates the SR Ca efflux channel, in a manner that could provide the positive feedback in Ca"-dependent Ca release.J. GEN. PHVstot. .

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“…Quercetin, a bioflavonoid, is known to increase Ca 2ϩ release from SR (Kirino and Shimizu, 1982;Watras et al, 1983;Kim et al, 1983;Palade et al, 1983). Increased Ca 2ϩ release by quercetin could be associated with its inhibitory effect on the Ca 2ϩ ATPase (Shoshan and MacLennan, 1981;Shoshan et al, 1980) as well as its stimulatory effect on CRC (Kim et al, 1983).…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, Kirino and Shimizu (1982) reported that quercetin stimulated Ca 2ϩ release from fragmented SR in the absence of extravesicular Ca 2ϩ , although they did not provide any direct evidence for a specific Ca 2ϩ efflux pathway. Watras et al (1983) demonstrated that quercetin increased Ca 2ϩ release from skeletal SR in the presence of 50 mM inorganic phosphate. Palade et al (1983) also showed an enhancement of spontaneous Ca 2ϩ release from SR by quercetin when the SR was loaded in the presence of 100 mM inorganic phosphate.…”

Section: Introductionmentioning

confidence: 96%

Effects of Quercetin on Single Ca2+ Release Channel Behavior of Skeletal Muscle

Lee

Meissner

Kim

2002

Biophysical Journal

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Quercetin, a bioflavonoid, is known to affect Ca(2+) fluxes in sarcoplasmic reticulum, although its direct effect on Ca(2+) release channel (CRC) in sarcoplasmic reticulum has remained to be elucidated. The present study examined the effect of quercetin on the behavior of single skeletal CRC in planar lipid bilayer. The effect of caffeine was also studied for comparison. At very low [Ca(2+)](cis) (80 pM), quercetin activated CRC marginally, whereas at elevated [Ca(2+)](cis) (10 microM), both open probability (P(o)) and sensitivity to the drug increased markedly. Caffeine showed a similar tendency. Analysis of lifetimes for single CRC showed that quercetin and caffeine led to different mean open-time and closed-time constants and their proportions. Addition of 10 microM ryanodine to CRC activated by quercetin or caffeine led to the typical subconductance state (approximately 54%) and a subsequent addition of 5 microM ruthenium red completely blocked CRC activity. When 6 microM quercetin and 3 mM caffeine were added together to the cis side of CRC, a time-dependent increase of P(o) was observed (from mode 1 (0.376 +/- 0.043, n = 5) to mode 2 (0.854 +/- 0.062, n = 5)). On the other hand, no further activation was observed when quercetin was added after caffeine. Quercetin affected only the ascending phase of the bell-shaped Ca(2+) activation/inactivation curve, whereas caffeine affected both ascending and descending phases. [(3)H]ryanodine binding to sarcoplasmic reticulum showed that channel activity increased more by both quercetin and caffeine than by caffeine alone. These characteristic differences in the modes of activation of CRC by quercetin and caffeine suggest that the channel activation mechanisms and presumably the binding sites on CRC are different for the two drugs.

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Quercetin stimulation of calcium release from rabbit skeletal muscle sarcoplasmic reticulum

Cited by 16 publications

References 39 publications

Characterization of increased Ca2+ efflux by quercetin from the sarcoplasmic reticulum in frog skinned skeletal muscle fibres

Characterization of increased Ca2+ efflux by quercetin from the sarcoplasmic reticulum in frog skinned skeletal muscle fibres

Excitation of skinned muscle fibers by imposed ion gradients. II. Influence of quercetin and ATP removal on the Ca2+-insensitive component of stimulated 45Ca efflux.

Effects of Quercetin on Single Ca2+ Release Channel Behavior of Skeletal Muscle

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