Quercetin treatment against ischemia/reperfusion injury in rat corpus cavernosum tissue: a role on apoptosis and oxidative stress

Abstract

Reactive oxygen metabolites play an important role in the ischemia/reperfusion (I/R)-induced tissue injury. This study was designed to investigate the possible protective effects of quercetin against I/R injury of the rat corpus cavernosum tissue. To induce I/R injury, abdominal aorta was clamped for 30 min and reperfused for 60 min. Quercetin (20 mg/kg) or vehicle was given before ischemia and just after reperfusion in the I/R group and in the sham-operated control group in which clamping was not performed. After decapitation, corpus cavernosum tissues were removed and either placed in organ baths or stored for evaluating biochemical parameters. Oxidative injury was examined by measuring lucigenin chemiluminescence (CL), nitric oxide (NO), malondialdehyde (MDA) and glutathione (GSH) levels, superoxide dismutase (SOD) and myeloperoxidase (MPO) activities and caspase-3 protein levels. In the I/R group, contractile responses to phenylephrine and relaxation responses to carbachol were impaired significantly compared with those in the control groups, while quercetin treatment in I/R group reversed both of the responses. On the other hand, increase in lucigenin CL, NO, MDA levels and MPO and caspase-3 activities and decrease in GSH levels and SOD activity in the cavernosal tissues of the I/R group were also significantly reversed by quercetin treatment. Furthermore, observed distorted morphology with ruptured endothelial cells and vacuolization in the cytoplasm of cavernosal tissues of I/R no longer persisted in the quercetin-treated I/R group. Thus, our results suggested that treatment with quercetin may have some benefits in controlling I/R-induced tissue injury through its anti-inflammatory, anti-apoptotic, and antioxidant effects.

Effect of chemotherapy exposure prior to pregnancy on fetal brain tissue and the potential protective role of quercetin

“…DOX-induced toxicity stimulates the translocation of calreticulin to the cell surface in tumor cells, enhancing uptake and clearance by dendritic cells (Bines et al 1996). Both in vitro and in vivo data support the hypothesis that a combination of the antioxidant Quercetin (QR) and DOX produces beneficial, synergistic effects in the treatment of breast cancer and leukemia (Black et al 2002;Bolzan et al 1997;Ceballos et al 1992) However, exposure of pregnant women to chemotherapy results in congenital malformations in as many as 10-20 % of cases (Cevik et al 2013).…”

Antioxidant Agent Quercetin Prevents Impairment of Bladder Tissue Contractility and Apoptosis in a Rat Model of Ischemia/Reperfusion Injury

“…Quercetin has been reported to inhibit oxidative damage in various I/R models and tissues, including myocardial, testicular and corpus cavernosum, but there are no studies against the I/R induced bladder injury . In our previous studies we have demonstrated the protective effects of quercetin (QT) against spinal cord injury‐induced bladder and I/R‐induced corpus cavernosum tissue damage and apoptosis, and suggested that this protection is through its antioxidant, anti‐apoptotic and anti‐inflammatory properties . Thus in the present study, we investigated the effect of QT on the I/R injury in the rat bladder first time in the literature, to the best of our knowledge.…”

“…In the sham‐operated control group, the abdominal aorta was left intact and the rats were treated with vehicle. The rationale for QT dose was based on previous studies showing anti‐oxidant effects of QT . At the end of the reperfusion period, 1 h after the second dose of s/c quercetin, rats were decapitated to obtain bladder tissue samples for in vitro organ bath experiments, biochemical analyses and histological examination.…”

Comparison of quercetin and resveratrol in the prevention of injury due to testicular torsion/detorsion in rats

“…According to previous studies, quercetin (QE) is a powerful antioxidant agent with the ability to reduce tissue damage after I/R processes 891011. Resveratrol (RSV) is found in many plants, particularly grapes and peanuts.…”