Questioning mechanisms such as Prime Minister’s Questions in the United Kingdom and Question Time in Australia are notoriously adversarial. Much less is known about whether and how questioning facilitates conflict in other legislatures. This question is particularly important given the criticism that excessive adversarialism may hinder the performance of accountability, and hence may be detrimental to the work of legislatures. Building on legislative studies literature, this article presents the first comparative study of conflict in oral parliamentary questions; in so doing, it explores patterns of conflictual remarks in questions addressed to prime ministers in Canada, Australia, the United Kingdom and Ireland. It posits that institutional culture, party discipline, government and opposition status and the authority of the Speaker are key factors in explaining the performance of conflict, and that rules of procedure alone are not enough to curb the manifestation of conflict in legislatures where questioning is a known opportunity for criticism.