Quick identification of kuraridin, a noncytotoxic anti-MRSA (methicillin-resistant Staphylococcus aureus) agent from Sophora flavescens using high-speed counter-current chromatography

Chan, Barbara Pui; Yu, Hua; Wong, Cheuk‐Yin; Lui, Sau Lai; Jolivalt, Claude; Ganem‐Elbaz, Carine; Paris, Jean‐Marc; Morleo, Barbara; Litaudon, Marc; Lau, Clara Bik‐San; Ip, Margaret; Fung, Kwok‐Pui; Leung, Ping‐Chung; Han, Quan‐Bin

doi:10.1016/j.jchromb.2011.11.039

Cited by 23 publications

(29 citation statements)

References 28 publications

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“…In our preliminary study, we found that the ethanol extract of PO itself showed weak inhibition against MRSA at 500 μg/ml, but could synergistically potentiate the antibacterial actions of some major classes of antibiotics [20]. High‐speed counter‐current chromatography (HSCCC) has been proven successful in tracking out active components from natural products effectively [21,22]. Hence, the aims of this study were to identify the active ingredients from PO that could provide synergisms with macrolides against MRSA using HSCCC coupled with gas chromatography mass spectrometry (GC‐MS), and to characterise their possible mechanisms of resistance inhibition.…”

Section: Introductionmentioning

confidence: 99%

“…[20] High-speed counter-current chromatography (HSCCC) has been proven successful in tracking out active components from natural products effectively. [21,22] Hence, the aims of this study were to identify the active ingredients from PO that could provide synergisms with macrolides against MRSA using HSCCC coupled with gas chromatography mass spectrometry (GC-MS), and to characterise their possible mechanisms of resistance inhibition.…”

Section: Introductionmentioning

confidence: 99%

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Combating against methicillin-resistant Staphylococcus aureus – two fatty acids from Purslane (Portulaca oleracea L.) exhibit synergistic effects with erythromycin

Chan

Han

Lui

et al. 2015

Journal of Pharmacy and Pharmacology

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Combating against methicillin-resistant Staphylococcus aureus – two fatty acids from Purslane (Portulaca oleracea L.) exhibit synergistic effects with erythromycin

Chan

Han

Lui

et al. 2015

Journal of Pharmacy and Pharmacology

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“…Previous study has reported the anti-microbial activities of SF 30 , 31 , however, the effect of SF on the antimicrobial property of macrophages has not been evaluated. In this study, an intracellular growing Mycobacterium aurum Tsukamura (M. aurum) (ATCC) strain was employed.…”

Section: Resultsmentioning

confidence: 99%

Sophora flavescens protects against mycobacterial Trehalose Dimycolate-induced lung granuloma by inhibiting inflammation and infiltration of macrophages

Liu

Chan

Cheng

et al. 2018

Sci Rep

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The immune system responds to Mycobacterium tuberculosis (MTB) infection by forming granulomas to quarantine the bacteria from spreading. Granuloma-mediated inflammation is a cause of lung destruction and disease transmission. Sophora flavescens (SF) has been demonstrated to exhibit bactericidal activities against MTB. However, its immune modulatory activities on MTB-mediated granulomatous inflammation have not been reported. In the present study, we found that flavonoids from Sophora flavescens (FSF) significantly suppressed the pro-inflammatory mediators released from mouse lung alveolar macrophages (MH-S) upon stimulation by trehalose dimycolate (TDM), the most abundant lipoglycan on MTB surface. Moreover, FSF reduced adhesion molecule (LFA-1) expression on MH-S cells after TDM stimulation. Furthermore, FSF treatment on TDM-activated lung epithelial (MLE-12) cells significantly downregulated macrophage chemoattractant protein (MCP-1/CCL2) expression, which in turn reduced the in vitro migration of MH-S to MLE-12 cells. In addition, FSF increased the clearance of mycobacterium bacteria (Mycobacterium aurum) in macrophages. FSF mainly affected the Mincle-Syk-Erk signaling pathway in TDM-activated MH-S cells. In TDM-induced mouse granulomas model, oral administration with FSF significantly suppressed lung granulomas formation and inflammation. These findings collectively implicated an anti-inflammatory role of FSF on MTB-mediated granulomatous inflammation, thereby providing evidence of FSF as an efficacious adjunct treatment during mycobacterial infection.

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“…Prenylated flavonoids have previously shown promise as antimicrobial agents [ 105 ]. Kuraridin (194) has shown promising activity against methicillin sensitive and resistant strains of S. aureus [ 106 ], but was inactive against E. coli [ 107 ]. Piperaduncin A (196) showed antibacterial activity against Bacillus subtilis and Micrococcus luteus , but was also inactive against E. coli [ 72 ].…”

Section: Resultsmentioning

confidence: 99%

The Search for Herbal Antibiotics: An In-Silico Investigation of Antibacterial Phytochemicals

2016

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Recently, the emergence and spread of pathogenic bacterial resistance to many antibiotics (multidrug-resistant strains) have been increasing throughout the world. This phenomenon is of great concern and there is a need to find alternative chemotherapeutic agents to combat these antibiotic-resistant microorganisms. Higher plants may serve as a resource for new antimicrobials to replace or augment current therapeutic options. In this work, we have carried out a molecular docking study of a total of 561 antibacterial phytochemicals listed in the Dictionary of Natural Products, including 77 alkaloids (17 indole alkaloids, 27 isoquinoline alkaloids, 4 steroidal alkaloids, and 28 miscellaneous alkaloids), 99 terpenoids (5 monoterpenoids, 31 sesquiterpenoids, 52 diterpenoids, and 11 triterpenoids), 309 polyphenolics (87 flavonoids, 25 chalcones, 41 isoflavonoids, 5 neoflavonoids, 12 pterocarpans, 10 chromones, 7 condensed tannins, 11 coumarins, 30 stilbenoids, 2 lignans, 5 phenylpropanoids, 13 xanthones, 5 hydrolyzable tannins, and 56 miscellaneous phenolics), 30 quinones, and 46 miscellaneous phytochemicals, with six bacterial protein targets (peptide deformylase, DNA gyrase/topoisomerase IV, UDP-galactose mutase, protein tyrosine phosphatase, cytochrome P450 CYP121, and NAD+-dependent DNA ligase). In addition, 35 known inhibitors were docked with their respective targets for comparison purposes. Prenylated polyphenolics showed the best docking profiles, while terpenoids had the poorest. The most susceptible protein targets were peptide deformylases and NAD+-dependent DNA ligases.

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Quick identification of kuraridin, a noncytotoxic anti-MRSA (methicillin-resistant Staphylococcus aureus) agent from Sophora flavescens using high-speed counter-current chromatography

Cited by 23 publications

References 28 publications

Combating against methicillin-resistant Staphylococcus aureus – two fatty acids from Purslane (Portulaca oleracea L.) exhibit synergistic effects with erythromycin

Combating against methicillin-resistant Staphylococcus aureus – two fatty acids from Purslane (Portulaca oleracea L.) exhibit synergistic effects with erythromycin

Sophora flavescens protects against mycobacterial Trehalose Dimycolate-induced lung granuloma by inhibiting inflammation and infiltration of macrophages

The Search for Herbal Antibiotics: An In-Silico Investigation of Antibacterial Phytochemicals

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