Quickly assessing disinfection effectiveness to control the spread of African swine fever virus

Zeng, Dexin; Qian, Bingxu; Li, Yunfei; Zong, Kai; Liu, Ding; Wang, Manman; Zhou, Tingting; Lv, Xiaying; Zhu, Kun Yan; Yu, Xiaofeng; Jiang, Yuan; Wu, Xiaodong; Xue, Feng; Dai, Jianjun

doi:10.1007/s00253-023-12611-3

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…ASFV is an enveloped virus so that some researchers have applied PMA pretreatments to identify infectious viruses from non-infectious ones. As shown in Table 1, Zeng et al (45) have developed a PMA-qPCR detection method for the rapid diagnosis of infectious ASFV and the evaluation of disinfection efficacy. This method exhibits a detection sensitivity of 10 1.28 HAD50/mL.…”

Section: Propidium Monoazide Qpcrmentioning

confidence: 99%

Current detection methods of African swine fever virus

Hu,

Tian,

Lai

et al. 2023

Front. Vet. Sci.

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African swine fever (ASF), caused by the African swine fever virus (ASFV), is a highly contagious and notifiable animal disease in domestic pigs and wild boars, as designated by the World Organization for Animal Health (WOAH). The effective diagnosis of ASF holds great importance in promptly controlling its spread due to its increasing prevalence and the continuous emergence of variant strains. This paper offers a comprehensive review of the most common and up-to-date methods established for various genes/proteins associated with ASFV. The discussed methods primarily focus on the detection of viral genomes or particles, as well as the detection of ASFV associated antibodies. It is anticipated that this paper will serve as a reference for choosing appropriate diagnostic methods in diverse application scenarios, while also provide direction for the development of innovative technologies in the future.

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Section: Propidium Monoazide Qpcrmentioning

confidence: 99%

Current detection methods of African swine fever virus

Hu,

Tian,

Lai

et al. 2023

Front. Vet. Sci.

View full text Add to dashboard Cite

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Rapidly quantification of intact infectious H1N1 virus using ICA-qPCR and PMA-qPCR

Liang,

Wang,

Fan

et al. 2024

Biosafety and Health

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Propidium Monoazide Integrated With qPCR Enables Rapid and Universal Detection of Infectious Porcine Reproductive and Respiratory Syndrome Viruses

Qi,

Qiu,

Zhao

et al. 2024

Transboundary and Emerging Diseases

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Infectious porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS, but noninfectious PRRSV cannot. PCR and ELISA are commonly used for PRRSV detection but they cannot discriminate PRRSV infectivity. Virus isolation is a gold standard to determine virus infectivity. However, it is time‐consuming. Therefore, we developed a propidium monoazide (PMA) qPCR assay for rapid and universal detection of infectious PRRSV in this study. After comparing the inactivation efficacies of distinct disinfectants, ultraviolet (UV) light, and heat, heat at 72°C for 15 min was determined as an effective strategy for PRRSV inactivation, which was confirmed by virus isolation and immunofluorescence assay (IFA) detection. In addition, PMA pretreatment parameters were optimized, including PMA concentration (5 μM), PMA binding time (25 min), PMA binding temperature (37°C), and photolysis time (25 min). The optimal concentration of primers and probes adapted from our previous study was redetermined. The optimized PMA‐qPCR assay exhibited satisfied specificity, sensitivity, and reproducibility. Furthermore, the new PMA‐qPCR was applied on the detection of 270 clinical samples (including 57 environmental feces, 177 lungs, 33 lymph nodes [LN], and 3 sera) and compared with previously developed qPCR. Eighty samples were qPCR positive, while only 63 samples were PMA‐qPCR positive. No virus could be isolated in the 17 qPCR‐positive but PMA‐qPCR‐negative clinical samples; meanwhile, PRRSV could be isolated in representative PMA‐qPCR‐positive samples, supporting that only live PRRSV isolates in distinct samples could be detected by this PMA‐qPCR assay. In conclusion, this study provides the first PMA‐qPCR assay for rapid and universal detection of infectious PRRSV, offering an alternative and effective method for PRRSV diagnosis, prevention, and control.

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Quickly assessing disinfection effectiveness to control the spread of African swine fever virus

Cited by 3 publications

References 24 publications

Current detection methods of African swine fever virus

Current detection methods of African swine fever virus

Rapidly quantification of intact infectious H1N1 virus using ICA-qPCR and PMA-qPCR

Propidium Monoazide Integrated With qPCR Enables Rapid and Universal Detection of Infectious Porcine Reproductive and Respiratory Syndrome Viruses

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