Quillaja brasiliensis saponin-based nanoparticulate adjuvants are capable of triggering early immune responses

Cibulski, Samuel Paulo; Rivera-Patron, Mariana; Mourglia‐Ettlin, Gustavo; Casaravilla, Cecilia; Yendo, Anna Carolina Alves; Fett-Neto, Arthur Germano; Chabalgoity, José A.; Moreno, Marı́a; Roehe, Paulo Michel; Silveira, Fernando

doi:10.1038/s41598-018-31995-1

Cited by 40 publications

(31 citation statements)

References 56 publications

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“…In parallel, QB saponins have emerged as a powerful alternative to QS saponin fractions. Indeed, QB-90, a QB fraction, has shown similar activity in comparison to Quil-A, when it is used in ISCOMs, promoting a local and transient "immunocompetent environment" (Cibulski et al, 2018). These findings are especially important for conservation and management of Quillaja species since alternative fractions from both species could be used for the development of new vaccines (Fleck et al, 2009;Costa et al, 2014;Cibulski et al, 2018).…”

Section: Bacteriamentioning

confidence: 98%

“…Nevertheless, Quillaja saponins could become unstable and induce undesirable hemolytic activity. To counteract those effects, saponins are normally used in combination with liposomes (Brunner et al, 2017) or with cholesterol and phospholipids to form a sort of self-assembled cage called the immunestimulating complex (ISCOM) (Papenmüller et al, 2014;Cibulski et al, 2018). Interestingly, the same affinity of Quillaja saponins to cholesterol is useful to fight cancer cells due to the higher membrane-cholesterol molecules content (Hu et al, 2010;Hassan et al, 2013).…”

Section: Use As Vaccine Adjuvantsmentioning

confidence: 99%

“…Quil-A adjuvant), are widely used for the development of animal vaccines. Their use in human vaccines is under evaluation (Cibulski et al, 2018;Gilabert-Oriol et al, 2015). QS21, the most purified fraction of the aqueous extract of QS, shows some problems, such as its low concentration, difficulty in extraction and purification and/or chemical instability (Adams et al, 2010;Brunner et al, 2017).…”

Section: Bacteriamentioning

confidence: 99%

“…Since the identification of saponins as the main metabolites in Quillaja species, many studies have investigated their composition and applications, mainly on QS and, to a lesser extent, on QB. In the latter, characterization of saponins and the analyses of their potential uses are relatively recent (Costa et al, 2014;Cibulski et al, 2018;Wallace et al, 2017Wallace et al, , 2019. In this review, we analyze historical aspects of the utilization of Quillaja species, and the progress in the characterization and applications of their saponins, incorporating general advances in the identification of saponin biosynthetic pathways, and related genes.…”

Section: Introductionmentioning

confidence: 99%

“…Unlike QS, QB leaves contain more saponins than bark (0.085 % vs 0.022 % of dry weight) (Magedans et al, 2019). The renewable use of bioactive saponins from QB leaves has gained more importance in recent years (Yendo et al, 2014;Costa et al, 2013Costa et al, , 2014Velazco et al, 2018;Cibulski et al, 2017Cibulski et al, -2018Fleck et al, 2009Fleck et al, , 2019Wallace et al, 2017Wallace et al, , 2019. According to the Brazilian National Center for Flora Conservation (CNCF, 2012), QB is an endangered species due to overgrazing and inadequate management, despite its economic importance.…”

Section: Introductionmentioning

confidence: 99%

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Saponin production from Quillaja genus species. An insight into its applications and biology

Guerra

Sepúlveda

2021

Sci. agric. (Piracicaba, Braz.)

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Quillaja genus (Quillajaceae family) is endemic to South America, where is represented by two species, Quillaja saponaria and Quillaja brasiliensis. One outstanding characteristic of these forest tree species is their production of saponins, a family of amphipathic glycosides, involved in the defensive response of plants against biotic and abiotic factors. Saponins are metabolites of economic importance due to their chemical and physical properties. Basic and applied research efforts performed during the last decades, mainly on Q. saponaria, have placed these compounds as an important raw material in industrial areas, such as food and beverage, cosmetics, vaccine production, biopesticides, among others. In this review, we summarize information on saponins from Quillaja species during the last years, analyzing current developments by application areas, as well as their chemical composition and properties. We also describe the general advances in revealing saponin biosynthesis pathways, related genes and Quillaja genomes, as well as the conservation status, domestication processes, and perspectives in the context of implementing genetic improvement programs.

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Section: Bacteriamentioning

confidence: 98%

Section: Use As Vaccine Adjuvantsmentioning

confidence: 99%

Section: Bacteriamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Saponin production from Quillaja genus species. An insight into its applications and biology

Guerra

Sepúlveda

2021

Sci. agric. (Piracicaba, Braz.)

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Potential therapeutic applications of phytoconstituents as immunomodulators: Pre‐clinical and clinical evidences

Ali

Singh

Datusalia

2021

Phytotherapy Research

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Autoimmune and infectious diseases are the major public health issues and have gained great attention in the last few years for the search of new agents with therapeutic benefits on the host immune functions. In recent years, natural products (NPs) have been studied broadly for their multi-targeted activities under pathological conditions. Interestingly, several attempts have been made to outline the immunomodulatory properties of NPs. Research on in-vitro and in-vivo models have shown the immunomodulatory activity of NPs, is due to their antiinflammatory property, induction of phagocytosis and immune cells stimulation activity. Moreover, studies on humans have suggested that phytomedicines reduce inflammation and could provide appropriate benefits either in single form or complex combinations with other agents preventing disease progression, subsequently enhancing the efficacy of treatment to combat multiple malignancies.However, the exact mechanism of immunomodulation is far from clear, warranting more detailed investigations on their effectiveness. Nevertheless, the reduction of inflammatory cascades is considered as a prime protective mechanism in a number of inflammation regulated autoimmune diseases. Altogether, this review will discuss the biological activities of plant-derived secondary metabolites, such as polyphenols, alkaloids, saponins, polysaccharides and so forth, against various diseases and their potential use as an immunomodulatory agent under pathological conditions.

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ISCOMs/MPLA‐Adjuvanted SDAD Protein Nanoparticles Induce Improved Mucosal Immune Responses and Cross‐Protection in Mice

Zhu

Park

Pho

et al. 2023

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The epidemics caused by the influenza virus are a serious threat to public health and the economy. Adding appropriate adjuvants to improve immunogenicity and finding effective mucosal vaccines to combat respiratory infection at the portal of virus entry are important strategies to boost protection. In this study, a novel type of core/shell protein nanoparticle consisting of influenza nucleoprotein (NP) as the core and NA1‐M2e or NA2‐M2e fusion proteins as the coating antigens by SDAD hetero‐bifunctional crosslinking is exploited. Immune‐stimulating complexes (ISCOMs)/monophosphoryl lipid A (MPLA) adjuvants further boost the NP/NA‐M2e SDAD protein nanoparticle‐induced immune responses when administered intramuscularly. The ISCOMs/MPLA‐adjuvanted protein nanoparticles are delivered through the intranasal route to validate the application as mucosal vaccines. ISCOMs/MPLA‐adjuvanted nanoparticles induce significantly strengthened antigen‐specific antibody responses, cytokine‐secreting splenocytes in the systemic compartment, and higher levels of antigen‐specific IgA and IgG in the local mucosa. Meanwhile, significantly expanded lung resident memory (RM) T and B cells (TRM/BRM) and alveolar macrophages population are observed in ISCOMs/MPLA‐adjuvanted nanoparticle‐immunized mice with a 100% survival rate after homogeneous and heterogeneous H3N2 viral challenges. Taken together, ISCOMs/MPLA‐adjuvanted protein nanoparticles could improve strong systemic and mucosal immune responses conferring protection in different immunization routes.

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Quillaja brasiliensis saponin-based nanoparticulate adjuvants are capable of triggering early immune responses

Cited by 40 publications

References 56 publications

Saponin production from Quillaja genus species. An insight into its applications and biology

Saponin production from Quillaja genus species. An insight into its applications and biology

Potential therapeutic applications of phytoconstituents as immunomodulators: Pre‐clinical and clinical evidences

ISCOMs/MPLA‐Adjuvanted SDAD Protein Nanoparticles Induce Improved Mucosal Immune Responses and Cross‐Protection in Mice

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