Quinacrine fluorescent karyotypes of human diploid and heteroploid cell lines

Miller, Orlando J.; Miller, Dorothy A.; Allderdice, P. W.; Dev, V.G.; Grewal, Manjit Singh

doi:10.1159/000130152

Cited by 93 publications

(27 citation statements)

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“…More recent CGH studies have revealed a high level copy increase of 5p to be a recurrent event during progression of advanced stage CaCx (Heselmeyer et al, 1997;Kirchhoff et al, 1999;Ried et al, 1999;Matthews et al, 2000). Similar markers have been observed in HPV-transformed cell lines (Miller et al, 1971;Durst et al, 1987;Popescu et al, 1987;James et al, 1989;Smith et al, 1989;Macville et al, 1999). Gain of 5p, however, is not observed in low-grade lesions or HR HPV containing keratinocyte cell lines at early passage in vitro (Cottage et al, 2001).…”

Section: Introductionmentioning

confidence: 64%

Amplification of chromosome 5p correlates with increased expression of Skp2 in HPV-immortalized keratinocytes

et al. 2003

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Section: Introductionmentioning

confidence: 64%

Amplification of chromosome 5p correlates with increased expression of Skp2 in HPV-immortalized keratinocytes

et al. 2003

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“…The donor cell line HeLa S3 contains at least three copies of human chromosome 17 (23). Therefore, the experimental values have also been standardized to the level of TK activity in the human microcell-mouse cell hybrid TMS5 (Table 3).…”

Section: Methodsmentioning

confidence: 99%

Chromosome-mediated gene transfer results in two classes of unstable transformants.

Klobutcher¹,

Miller²,

Ruddle³

1980

Proc. Natl. Acad. Sci. U.S.A.

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“…Suspension cultures of Wil2 lymphocytes (4,5) were grown in Auto-Pow BME medium supplemented with 10% fetal calf serum and other ingredients as described previously (2). Normal human fibroblasts initiated from human foreskin were obtained from Dr with 20% fetal calf serum.…”

mentioning

confidence: 99%

Localization of cytoplasmic-membrane-associated DNA in human chromosomes.

Kuo¹,

Meinke²,

Saunders³

1975

Proc. Natl. Acad. Sci. U.S.A.

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In situ hybridization was used to localize the sites of the repeated sequences of cytoplasmic-membrane-associated DNA (cmDNA) in human chromosomes. cmDNA was mainly present in the heterochromatic regions of chromosomes 9. Although cmDNA has buoyant densities in neutral and alkaline CsCl gradients similar to those of human satellite C DNA, which also is localized in the heterochromatic region of chromosomes 9, cmDNA was not homologous to satellite C DNA as determined by DNA'complementary RNA hybridization. These results are in agreement with our previous studies, which indicated that cmDNA is a unique species of human DNA.Cytoplasmic-membrane-associated DNA (cmDNA), isolated from human lymphocytes, has been shown to possess physical and chemical properties which differ from those of chromosomal and mitochrondrial DNAs (1). Reassociation kinetics of cmDNA show that this DNA is composed of two fractions with distinct reassociation rates (2). About 70% of cmDNA reassociates rapidly with a C_0t12 of 2.3 X 10-2 mol-sec/liter and the remaining 30% reassociates with a C0t1/2 of 223. The fast reassociating fraction is homologous to approximately 4% of the repetitive sequences of nuclear DNA, while the more slowly reassociating fraction hybridizes with 11% of the unique sequences of nuclear DNA (2).It has been suggested that cmDNA is synthesized in the nucleus and subsequently "transported" to associate with cytoplasmic membranes (3). If cmDNA represents a selected class of sequences in the human genome, then chromosome localization of at least the repetitive portion of cmDNA may indicate its origin. Alternatively, cmDNA may contain repetitive sequences similar to those found in all chromosomes and give an ambiguous chromosomal localization pattern. In this report, localization was accomplished by employing the complementary RNA with 20% fetal calf serum. Colcemid (0.01 ,ug/ml) was used to arrest the cells 2 hr before harvest.(b) DNA Extraction and Fractionation. cmDNA was isolated from lysed cells as described previously (2). cmDNA was sheared to an average molecular weight of 2 X 105 and allowed to reassociate to a Cot value of 5. The reassociated DNA, isolated by hydroxyapatite fractionation, is termed repetitive cmDNA (2). DNA was dialyzed against distilled H20 and then lyophilized. Complementary RNA was synthesized from DNA prepared in this manner. Satellite C DNA, isolated as described previously (6)

show abstract

Quinacrine fluorescent karyotypes of human diploid and heteroploid cell lines

Cited by 93 publications

References 1 publication

Amplification of chromosome 5p correlates with increased expression of Skp2 in HPV-immortalized keratinocytes

Amplification of chromosome 5p correlates with increased expression of Skp2 in HPV-immortalized keratinocytes

Chromosome-mediated gene transfer results in two classes of unstable transformants.

Localization of cytoplasmic-membrane-associated DNA in human chromosomes.

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