Quinine sulfate and bacterial invasion

Abstract

BackgroundAs many patients who receive antimalarial drugs for treatment of noninfectious, inflammatory diseases are also immunosuppressed and might have a concomitant bacterial infection, we studied the effectiveness of these drugs against bacterial infections, to find out whether they could protect against (and even treat) such conditions and obviate the need for an additional antibiotic drug.MethodsEffect of QS on bacterial growth: Escherichia coli (E. coli) HB101 pRI203 were cultured overnight at 37°C in TSB and inoculated (approx 1 × 107 cells /ml) in MEM in the presence of QS at various concentrations (0, 50 and 100 μM).The effect of QS at concentration of 50 and 100 μM on the entry process of E. coli HB101 pRI203 into HeLa cells was studied under different experimental conditions: 1. QS was incubated with 3 × 105 HeLa cells for 60 min at 37°C prior to infection. 2. QS was added to HeLa cell monolayers during the infection period.ResultsQS showed no antibacterial activity after 24 h of incubation.The invasive efficiency of the bacteria was significantly inhibited at a dose-dependent manner, when QS was added to HeLa cells for 60 min at 37°C prior to infection (condition 1), and to a lesser extent when added during the period of infection (condition 2).ConclusionsAlthough the antimalarials are generally regarded as being inactive against most extracellular bacterial species, our results indicate that QS significantly inhibited the internalization/invasion efficacy of E. coli in the host cells.

Inhibition of Toxoplasma gondii Growth by Dihydroquinine and Its Mechanisms of Action

“…host cell penetration ( Fayer et al., 1972 ). Additionally, in prokaryotic cells (i.e., bacteria), quinine derivative (quinine sulfate) has been found to block bacteria invasion and internalization ( Wolf et al., 2002 ; Wolf et al., 2006 ).…”

“…host cell invasion ( Fayer et al., 1972 ). Interestingly quinine sulfate, a derivative of quinine, exhibited a strong inhibition of skin pathogenic bacteria invasion and internalization of host cells ( Wolf et al., 2002 ; Wolf et al., 2006 ). Interestingly, DHQ is an impurity often associated with quinine drugs formulations constituting up to 10% and has potent activity against Plasmodium species ( Nontprasert et al., 1996 ).…”

Multifunctional Therapeutic Potential of Phytocomplexes and Natural Extracts for Antimicrobial Properties

“…Curcumin exerts anti-inflammatory activity by inhibiting the inflammatory response of TNF-α stimulated human endothelial cells by interfering with NF-κB. Additionally, curcumin inhibits platelet-derived growth factor (PDGF) [181].…”

Quinoline Antimalarials Increase the Antibacterial Activity of Ampicillin

“…Of more interest is the fact that chloroquine and quinine have been reported to have some antibacterial activity, albeit at high concentrations, arising from their structural similarities to (Wolf et al, 2002;Lv et al, 2007;Davidson et al, 2008;Kharal et al, 2009;Bawa et al, 2010;Achan et al, 2011;Jagadeesh et al, 2014) quinolone antibacterials.…”