Quinine Sulfate as a Fluorescence Quantum Yield Standard

Fletcher, A. N.

doi:10.1111/j.1751-1097.1969.tb07311.x

Cited by 190 publications

(127 citation statements)

References 11 publications

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“…Integral extinction coefficients were determined as a ratio of pro tein absorption and its total concentration. Fluorescence quantum yields were determined by comparative method [28] with rhodamine 6G as a standard (quantum yield in ethanol 0.95 [29]). …”

Section: Methodsmentioning

confidence: 99%

Mutants of monomeric red fluorescent protein mRFP1 at residue 66: Structure modeling by molecular dynamics and search for correlations with spectral properties

Khrameeva

Друца

Vrzheshch

et al. 2008

Biochemistry Moscow

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Fluorescent proteins are widely used as markers for visualization of processes in intact biological systems. The family of fluorescent proteins includes proteins of various representatives of Coelenterata that are able to absorb light and emit in the visible spectral range. All flu orescent proteins have similar structure represented by 11 strand β barrel with α helix inside, which contains a chromophore in the middle. The chromophore is a het erogroup formed by an autocatalytic posttranslational reaction between three adjacent amino acid residues. The presence of the chromophore imparts color to the protein and induces its possible fluorescence [1].Fluorescence of proteins of this family is influenced by external conditions: pH [2,3], temperature [1], and ionic content of the medium [4,5], but structures of the chromophore and its nearest environment [1,6] are the key factors. The representatives of this family in matura tion rate, stability, spectral properties (absorption and flu orescence spectra, fluorescence quantum yield, photo bleaching, etc.) are described in [1,7]. Theoretical, experimental, and computational studies are carried out to investigate the mechanism of fluorescence of these proteins [8 11].Green fluorescent protein (GFP) was discovered in the early 1960s [12], but its active study began only after cloning of the GFP gene in 1992 [13] and demonstration of its heterologic expression in other organisms. In 1999, another family of colored proteins including the red pro tein DsRed was cloned from corals [14]. However, DsRed is a tetramer, and its use as a fluorescent marker is limit ed because of possible effects of its large molecular mass ISSN 0006 2979, Biochemistry (Moscow), 2008, Vol. 73, No. 10, pp. 1085 1095. © Pleiades Publishing, Ltd., 2008. Original Russian Text © E. E. Khrameeva, V. L. Drutsa, E. P. Vrzheshch, D. V. Dmitrienko, and P. V. Vrzheshch, 2008, published in Biokhimiya, 2008, Vol. 73, No. 10, pp. 1355 1367. Originally published in Biochemistry (Moscow) On Line Papers in Press, as Manuscript BM08 038, September 21, 2008 1085 Abbreviations: DsRed) red fluorescent protein drFP583 from coral Discosoma sp.; GFP) green fluorescent protein from Aequorea victoria; MD) molecular dynamics; mRFP1) monomeric red fluorescent protein, mutant of DsRed; Q66X) mutants of mRFP1 with replacement of residue 66 by residue X. * To whom correspondence should be addressed. Abstract-To study the interrelation between the spectral and structural properties of fluorescent proteins, structures of mutants of monomeric red fluorescent protein mRFP1 with all possible point mutations of Glu66 (except replacement by Pro) were simulated by molecular dynamics. A global search for correlations between geometrical structure parameters and some spectral characteristics (absorption maximum wavelength, integral extinction coefficient at the absorption maximum, excitation maximum wavelength, emission maximum wavelength, and quantum yield) was performed for the chromophore and its 6 A environment in mRFP1, Q66A, Q...

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Section: Methodsmentioning

confidence: 99%

Mutants of monomeric red fluorescent protein mRFP1 at residue 66: Structure modeling by molecular dynamics and search for correlations with spectral properties

Khrameeva

Друца

Vrzheshch

et al. 2008

Biochemistry Moscow

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“…The photophysics of QS have been studied extensively because of its medical applications as well as its use as a fluorescence standard. 20,21 The stability of the inherent fluorescence of QS is attested by its acceptance as one of the most commonly used fluorescence reference standards for monitoring relative fluorescence intensities. Previous studies also showed that quinine could intercalate into DNA, disrupting the parasite's replication and transcriptions, 22,23 but the determination of DNA using QS as a probe with fluorescent techniques have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Study on the Interaction between Quinine Sulfate and DNA by Multiple Spectral Methods and Their Analytical Applications

et al. 2013

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“…The bandwidth of the excitation monochromator was kept smaller than 2 nm to avoid an influence of the spectral slit width of the entrance slit on the fluorescence yields [12,13]. Four samples were measured during each experiment according to the following procedure:…”

Section: Methodsmentioning

confidence: 99%

Fluorescence Quantum Yields of Laser Dyes Determined by On-Line Computer Evaluation

Gauglitz

Lorch

Oelkrug

1982

Zeitschrift Für Naturforschung A

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The determination of relative fluorescence quantum yields has been improved to better than 3% precision by use of a multichannel analyzer and an on-line desk-top computer. The new combined device for data acquisition and handling as well as a new correction procedure are described. Drifts of excitation source and dark current can be omitted. The yields for laser dyes, emitting at short wavelengths, are presented in dependence on excitation wavelength. These results simplify the optimal choice of the pump conditions of laser dyes, which usually are not photostable.

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Quinine Sulfate as a Fluorescence Quantum Yield Standard

Cited by 190 publications

References 11 publications

Mutants of monomeric red fluorescent protein mRFP1 at residue 66: Structure modeling by molecular dynamics and search for correlations with spectral properties

Mutants of monomeric red fluorescent protein mRFP1 at residue 66: Structure modeling by molecular dynamics and search for correlations with spectral properties

Study on the Interaction between Quinine Sulfate and DNA by Multiple Spectral Methods and Their Analytical Applications

Fluorescence Quantum Yields of Laser Dyes Determined by On-Line Computer Evaluation

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