Quinoa whole grain diet compromises the changes of gut microbiota and colonic colitis induced by dextran Sulfate sodium in C57BL/6 mice

Abstract

A plethora of evidence highlights that the dysbiosis of gut microbiota is a critical factor for inflammatory bowel disease (IBD). Both in vivo and in vitro studies have demonstrated that quinoa possesses potential prebiotic effects. The present study aims to examine the potential in using quinoa to ameliorate the dysbiosis and colitis induced by dextran sodium sulfate (DSS). A total of 40 C57BL/6 mice were fed either an AIN-93M diet or a quinoa-based diet, separately. Colitis was induced for 10 animals/dietary group with a 5-days exposure to 2.5% DSS. The clinical symptoms were monitored every other day, and the gut microbiota was characterized by 16S rRNA gene sequencing. The results indicated that consumption of quinoa lessened clinical symptoms as indicated by the reduced disease activity index and the degree of histological damage (P < 0.05). As expected, the DSS treatment induced significant dysbiosis of gut microbiota in mice on an AIN-93M diet. However, compared to mice fed the AIN-93M diet, the consumption of quinoa alleviated the DSS-induced dysbiosis remarkably, as indicated by increased species richness and diversity, decreased abnormal expansion of phylum Proteobacteria, and decreased overgrowth of genera Escherichia/Shigella and Peptoclostridium (P < 0.05). The relative abundances of Firmicutes and Bacteroidetes were less altered in mice fed with quinoa comparing to those mice fed the AIN-93M diet. In summary, the consumption of quinoa suppressed the dysbiosis of gut microbiota and alleviated clinical symptoms induced by DSS, indicating the potential to utilize quinoa as a dietary approach to improve intestinal health.

Sishen Pill Treatment of DSS-Induced Colitis via Regulating Interaction With Inflammatory Dendritic Cells and Gut Microbiota

“…The enrichment of Lachnospiraceae _NK4A136 (around 3.5 folds) was identified in the DSS group compared with the normal group (around 3.0 folds of increase). Findings from current studies of Lachnospiraceae _NK4A136 abundance in IBD model are still a controversy ( Xiao et al, 2016 ; Liu et al, 2018 ; Li M. et al, 2019 ; Li P. et al, 2019 ). Some of them illustrated that an increased abundance of Lachnospiraceae _NK4A136 contributes to alleviate inflammation in IBD model; however, that is not supported by our observation in the present study ( Liu et al, 2018 ; Li M. et al, 2019 ; Li P. et al, 2019 ).…”

Autoinducer-2 May Be a New Biomarker for Monitoring Neonatal Necrotizing Enterocolitis

“…also reported that, a high-grain diet dynamically increased the relative abundance of Clostridium_sensu_stricto_1 and induced mucosal injuries in the colon of sheep. Quinoa consumption has been reported (Liu et al, 2018) to significantly alleviate DSSinduced dysbiosis as indicated by decreased abnormal expansion of the phylum Proteobacteria and decreased overgrowth of the genus Peptoclostridium. We also found that the abundances of Enterococcus, Peptoclostridium, and Veillonella were significantly different between the antibiotics-free group and the control group, which indicated that antibiotics have a great influence on the intestinal flora of newborns.…”

Linggui Zhugan Formula Improves Glucose and Lipid Levels and Alters Gut Microbiota in High-Fat Diet-Induced Diabetic Mice

“…In addition to changes in specific taxa, the richness and diversity of gut microbiota are often significantly altered by HF diets (Daniel et al, 2014; Liu et al, 2018) or different drug interventions (Li et al, 2017; Song et al, 2017). The gut microbiota is a major environmental factor that can influence metabolic modifications in the host organism (Ley et al, 2006); it is involved in very important aspects of metabolism, such as vitamin metabolism (Ramakrishna, 2013), SCFA metabolism (Kimura et al, 2013), neuropeptide response (Frohlich et al, 2016), and food digestion.…”