Quinoline biodegradation and its nitrogen transformation pathway by a Pseudomonas sp. strain

Abstract

A Pseudomonas sp. strain, which can utilize quinoline as its sole carbon, nitrogen and energy source, was isolated from activated sludge in a coking wastewater treatment plant. Quinoline can be degraded via the 8-hydroxycoumarin pathway. We quantified the first two organic intermediates of the biodegradation, 2-hydroxyquinoline and 2,8-dihydroxyquinoline. We tracked the transformation of the nitrogen in quinoline in two media containing different C/N ratios. At least 40.4% of the nitrogen was finally transformed into ammonium when quinoline was the sole C and N source. But addition of an external carbon source like glucose promoted the transformation of N from NH3 into NO3(-), NO2(-), and then to N2. The product analysis and gene characteristics indicated that the isolate accomplished heterotrophic nitrification and aerobic denitrification simultaneously. The study also demonstrated that quinoline and its metabolic products can be eliminated if the C/N ratio is properly controlled in the treatment of quinoline-containing wastewater.

Biodegradation of carbazole by the seven Pseudomonas sp. strains and their denitrification potential

“…The Luria-Bertani (LB) medium [11] was used for bacteria enrichment and maintenance. The mineral salt medium (MSM), which does not contain N source as described by Bai et al [12], was used as the basic ingredient for the bacterial degradation and transformation. The carbazole was dissolved in DMSO (30 g/L) and added to the MSM as the sole degradable carbon, nitrogen, and energy source in the biodegradation experiments.…”

Biodegradation and Interaction of Quinoline and Glucose in Dual Substrates System

“…Quinoline and its derivates are found widely in coal tar, oil shale, and petroleum, and serve as intermediates and solvents in many industries (Bai et al 2010). The existence of these compounds has a great negative impact on human health and environmental quality because of the toxic, mutagenic and carcinogenic properties of these compounds.…”

Rapid Determination of Quinoline and 2-Hydroxyquinoline in Quinoline Biodegradation Process by Tri-Wavelength UV/Vis Spectroscopy

“…3,4 In previous studies, [4][5][6] it was found that certain bacteria are effective in decomposing Q. It was also suggested that the biochemical conversion of Q into 2-hydroxyquinoline (HQ) 7,8 occurs in the first step of the metabolic pathways that are affected by the intermediates. 9 However, the mechanisms of Q degradation in bacteria remain unclear due to lack of quantitative information about intermediate and final products of the biodegradation.…”

“…3,7,[10][11][12] However, sophisticated or intensive sample pretreatment procedures are required for using these methods. For example, sample filtration must be used to remove the precipitates or particles from the sample solution before HPLC analysis.…”