Quinolone-resistant<i>Campylobacter</i>Infections: Risk Factors and Clinical Consequences1

Engberg, Jørgen; Neimann, J.; Nielsen, Eva Møller; Aarestrup, Frank Møller; Fussing, Vivian

doi:10.3201/eid1006.030669

Cited by 151 publications

(163 citation statements)

References 24 publications

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“…strains isolated from poultry reveals the impact of the use of these antibiotics in poultry production (ENDTZ et al, 1991;SMITH et al, 1999;ENGBERG et al, 2004;HUMPHREY et al, 2005). Resistance levels vary according to the country, variable factors including the permission to use this drug in poultry production (CHEN et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…The same occurred in the United Kingdom and the United States after the approval of its use in Veterinary Medicine (NACHAMKIN et al, 2002). The acquisition of resistance affects the treatment of patients, increasing the duration of gastrointestinal symptoms in patients infected with resistant strains to fluoroquinolones (SMITH et al, 1999;ENGBERG et al, 2004).…”

Section: Microbiologymentioning

confidence: 99%

See 1 more Smart Citation

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Section: Microbiologymentioning

confidence: 99%

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

et al. 2015

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“…Moreover, by mechanisms yet to be elucidated, fluoroquinolone resistance appears to coincide with increased fitness of the organism. In humans, fluoroquinolone-resistant strains are shed longer than sensitive isolates (Nelson et al, 2004;Engberg et al, 2004), and they have been reported to out-compete their sensitive counterparts, at least in certain genetic backgrounds, when colonizing poultry, i.e. the natural reservoir of the pathogen (Luo et al, 2005).…”

mentioning

confidence: 99%

High level of ciprofloxacin resistance and its molecular background among Campylobacter jejuni strains isolated in the United Arab Emirates

Sonnevend

Rotimi

Kolodziejek

et al. 2006

Journal of Medical Microbiology

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The antibiotic sensitivity and the serotype and molecular type (MT) distribution of 41 Campylobacter jejuni strains isolated from individual patients in Tawam Hospital, Al Ain, United Arab Emirates, were investigated. While all strains were sensitive to erythromycin (MIC 0?5-4 mg l "1 ), 35 isolates (85?4 %) exhibited resistance to ciprofloxacin (MIC 8-64 mg l "1 ). All resistant strains carried the Thr-86 to Ile mutation in the gyrase A (gyrA) gene, as shown by mismatch amplification mutation assay (MAMA) and confirmed by sequencing. Based on the partial sequences of gyrA, resistant isolates carried 10 distinct alleles, eight of them representing new variants. Strains were assigned to 30 MTs based on the combined results of PFGE and flaA PCR-RFLP typing. Eight of the 35 ciprofloxacin-resistant strains, isolated over a period of more than 1 year, represented the largest MT, also carrying the same allelic variant of the gyrA gene. These results show that the local incidence of fluoroquinolone resistance among C. jejuni is one of the highest reported worldwide. It was also demonstrated that stable MTs could persist for a relatively long time among the clonally unrelated antibiotic-resistant isolates of C. jejuni. The data also emphasize the need to replace fluoroquinolones as empirical therapy for diarrhoea of undiagnosed aetiology. INTRODUCTIONFluoroquinolone resistance in Campylobacter jejuni, a leading cause of gastrointestinal infections worldwide and one of the most important causes of travellers' diarrhoea, is rapidly increasing. Resistance is commonly due to point mutations in the quinolone resistance determinant region (QRDR) of the gyrase A (gyrA) gene involving the Thr-86 for high, and the Asp-90 and Ala-70 amino acid residues for lower-level resistance (Engberg et al., 2001).While C. jejuni infections usually present as acute selflimiting gastrointestinal illnesses, in severe or prolonged cases antibiotic therapy is indicated, with macrolides as the primary drug of choice (Butzler, 2004). However, particularly if the causative agent is not identified, as is often the case in travellers' diarrhoea, fluoroquinolones are frequently used empirically (Guerrant et al., 2001;Yates, 2005). In such cases, resistance to ciprofloxacin can result in therapeutic failure (Guerrant et al., 2001;Sanders et al., 2002). Moreover, by mechanisms yet to be elucidated, fluoroquinolone resistance appears to coincide with increased fitness of the organism. In humans, fluoroquinolone-resistant strains are shed longer than sensitive isolates (Nelson et al., 2004;Engberg et al., 2004), and they have been reported to out-compete their sensitive counterparts, at least in certain genetic backgrounds, when colonizing poultry, i.e. the natural reservoir of the pathogen (Luo et al., 2005). All these findings underscore the necessity for close monitoring of the incidence and spread of fluoroquinolone-resistant clones of C. jejuni. Campylobacter isolates recovered from the 3389 stool samples tested during this period of time. Seven of th...

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“…Engberg et al (2004) reported 2% C. jejuni and 18.5% C. coli strains resistant to erythromycin. Data concerning such a high level of resistance to erythromycin in Campylobacter strains isolated from poultry are surprising having in mind that Denmark is a developed country in which the use of antibiotics is limited to therapeutic indications only.…”

Section: Discussionmentioning

confidence: 99%

Resistance to erythromycin of Campylobacter jejuni and Campylobacter coli isolated from animals and humans

Tambur¹,

Miljković-Selimović²,

Kulišić³

et al. 2011

Afr. J. Pharm. Pharmacol.

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The sensititivity of thermophilic Campylobacter strains isolated from caecum of broiler chickens as well as caecum and colon of pigs and human stools, were tested against erythromycin. In 16 strains isolated in broiler chickens, resistance rate was found to be 12.50%. Three of 10 strains of Campylobacter jejuni and one of 6 strains of Campylobacter coli isolated from broiler chickens were resistant to erythromycin. In 15 strains of thermophilic Campylobacters isolated from pigs, resistance rate to erythromycin was 40.00%. Resistance was exhibited more often in C. coli (50.00%) as compared to C. jejuni (20.00%). In 24 strains isolated from humans, resistance was demonstrated at the rate of 12.50%. Out of 17 strains of C. jejuni isolated from humans, resistance was exhibited in 17.65% strains. None of 7 strains of C. coli isolated from humans exhibited resistance to erythromycin. Thermophilic campylobacters, especially C. coli isolated from pigs were more resistant to erythromycin than strains isolated from humans and broiler chickens. Therefore, a great attention should be directed to the macrolides monitoring in swine farming in order to prevent resistance in animals and its subsequent spread to human.

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Quinolone-resistantCampylobacterInfections: Risk Factors and Clinical Consequences1

Cited by 151 publications

References 24 publications

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

Detection of fluoroquinolone resistance by mutation in gyrA gene of Campylobacter spp. isolates from broiler and laying (Gallus gallus domesticus) hens,from Rio de Janeiro State, Brazil

High level of ciprofloxacin resistance and its molecular background among Campylobacter jejuni strains isolated in the United Arab Emirates

Resistance to erythromycin of Campylobacter jejuni and Campylobacter coli isolated from animals and humans

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