Quinolones Resistance And R-Plasmids Of Clinical Isolates Of &lt;i&gt;Pseudomonas&lt;/i&gt; Species

Daini, OA; Effiong, MJ; Ogbolu, OD

doi:10.4314/sjms.v3i2.38528

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…Several previous studies carried out in different settings also support the multi-drug resistance pattern of Pseudomonas spp. 24 – 26 . The mechanisms of intrinsic resistance of Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of antibiotic susceptibility in wound infections: A pilot study from Bangladesh

Roy¹,

Ahmed²,

Uddin³

et al. 2017

F1000Res

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Introduction: Infections due to antibiotic resistant bacteria have increased alarmingly in both developed and developing countries. Unrestrained and rapidly spreading bacterial growth has turned the management of wound infections into a serious challenge. This study aimed to determine the prevalence of different bacterial pathogens and their antibiotic susceptibility in various types of wound infections. Methods: A cross-sectional study was conducted to collect 105 wound swabs. All isolated bacteria were identified based on colony characteristics, gram stain and standard biochemical tests, and antibiotic susceptibility testing (AST) with the disc diffusion method. Descriptive statistics were used to present the study findings, and all analyses were performed using Stata Version 13. Results: The rate of isolation of bacteria was 92.3%. Staphylococcus aureus was found to be the most frequent isolate (55.7%), followed by Escherichia coli (23.7%), Pseudomonas spp. (8.2%), and Streptococcus pyogenes (7.2%). Gram-positive bacteria were mostly (60%) found sensitive to vancomycin, azithromycin, gentamicin, imipenem, cefixime, and ceftriaxone in this study. Among the Gram-negative bacteria, Escherichia coli (>60%) showed sensitivity to cefixime, azithromycin, cefuroxime, ceftriaxone, cefotaxime, gentamycin, and ceftazidime. Conclusions: The diversity of isolated bacteria and their susceptibility patterns signify a need to implement a proper infection control strategy, which can be achieved by carrying out antibiotic sensitivity tests of the isolates.

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Section: Discussionmentioning

confidence: 99%

Evaluation of antibiotic susceptibility in wound infections: A pilot study from Bangladesh

Roy¹,

Ahmed²,

Uddin³

et al. 2017

F1000Res

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show abstract

“…The emergence of MDR and ESBL E. coli pose antibiotic management problems (Lim et al, 2009). Multiple antibiotic resistances in bacteria and production of ESBL is most commonly associated with the presence of plasmids which contain one or more resistance genes, each coding a single antibiotic resistance phenotype (Paterson et al, 2005;Daini et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Extended Spectrum Beta Lactamases Detection and Multiple Antibiotic Resistance Indexing of Escherichia Coli from Urine Samples of Patients from a Referral Hospital of Eastern Nepal

Chakrawarti¹,

Dongol²,

Khanal³

et al. 2015

Int J Appl Sci Biotechnol

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Background: Escherichia coli is the most common causative agent of urinary tract infection. Antibiotic resistance among uropathogens has become a prominent public health problem. Multidrug resistance bacteria have limited the therapeutic possibilities by producing Extended Spectrum Beta Lactamases (ESBL). Objective: Since routine monitoring of ESBL producers are not conducted in clinical laboratories their true prevalence is still unknown. So the objective of this research was to assess multiple antibiotic resistance (MAR) indices and determine ESBL production among Escherichia coli isolated from urine samples. Methods: Standard microbiological techniques and antibiotic sensitivity test were performed by Kirby Bauer disc diffusion method to identify E. coli. ESBL screening was done by using Ceftriaxone, Aztreonam, Cefotaxime, Ceftazidime and Cefpodoxime whereas confirmation by combined disc assay. SPSS 16 software was used to analyze data. Results: 86.95% E. coli isolates were MDR strains. 27 isolates had multiple antibiotic resistance (MAR) index of 0.2 and 5 isolates had MAR index of 0.7. E. coli isolates showed higher degree of resistance towards Amoxicillin (100%) while 100% were sensitive towards Gentamicin followed by Nitrofurantoin (62.31%). The reliable screening agent for ESBL detection with sensitivity 100% and positive predictive value of 80% was Cefotaxime. Combined disc assay detected 12/69 (17.31%) of E. coli isolates as confirmed ESBL producers. Conclusion: The ubiquity of ESBL-producing E. coli was observed emphasizing the necessity of regular surveillance of ESBL producing clinical isolates in clinical samples to minimize multi-drug resistance strains and avert the ineffectiveness of antimicrobial agent for good health practices.\Int J Appl Sci Biotechnol, Vol 3(3): 423-426

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“…The types of antibiotics and their frequency of usage in the various patients from whom the specimens were taken may be the cause of the diversity in the resistance pattern of P. vulgaris isolates. However, [21] found that all Proteus isolates were sensitive to ciprofloxacin whereas [22] revealed that the isolates of P. vulgaris were resistant to ciprofloxacin. [23] showed that (80%)…”

Section: Resultsmentioning

confidence: 99%

Determining the Occurrence of Some Virulence Genes in Proteus Species Isolates

Naji,

Hassan

2023

JLSAR

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Forty isolates of Gram-negative rod-shaped bacteria termed as Proteus, widely known for their swarming motility and urease activity, which cause complicated urinary tract infections (UTIs), were isolated and identified. Two hundred and ten urine specimens collected from the patients suffering from UTIs, who were hospitalized in Babylon hospitals, were used for the isolation of Proteus species isolates. The morphological features (for cells and colonies), biochemical tests, VITEK 2 compact and polymerase chain reaction (PCR) for Proteus-specific genes were used for the identification of these isolates. The assessment of the antimicrobial profiles that represent the prevalence and the level of pathogenicity of the isolates was also carried out. Furthermore, the whole genomic DNA of the isolates was extracted to determine the sites of blaTEM, blaCTX-M, and blaSHV genes on the genome of the isolates. The results revealed that thirty isolates were P. mirabilis and ten isolates were P. vulgaris. These isolates were given names as PM1 to PM30 for P. mirabilis and PV31 to PV40 for P. vulgaris. The most effective antibiotics against the isolates were erythromycin (97.5%), followed by tobramycin (85%), ampicillin (82.5%), chloramphenicol (60%), piperacillin (55%) and 52.5% for each sulfamethoxazole and azithromycin. The meropenem and imipenem showed less resistance (35%) followed by ciprofloxacin (30%) and gentamicin (15%). The PCR assay exhibited that these isolates carried blaTEM gene at the rate of 38/40 (95%), blaSHV gene at the rate of 33/40 (82.5%) and blaCTX-M gene at the rate of 37/40 (92.5%). Therefore, reducing the frequency and severity of infections, however, more research is needed to understand how the rates of pathogenicity of Proteus species isolates can be controlled.

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Quinolones Resistance And R-Plasmids Of Clinical Isolates Of <i>Pseudomonas</i> Species

Cited by 7 publications

References 14 publications

Evaluation of antibiotic susceptibility in wound infections: A pilot study from Bangladesh

Evaluation of antibiotic susceptibility in wound infections: A pilot study from Bangladesh

Extended Spectrum Beta Lactamases Detection and Multiple Antibiotic Resistance Indexing of Escherichia Coli from Urine Samples of Patients from a Referral Hospital of Eastern Nepal

Determining the Occurrence of Some Virulence Genes in Proteus Species Isolates

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