Quorum Sensing and Quorum Quenching in Agrobacterium: A “Go/No Go System”?

Dessaux, Yves; Faure, Denis

doi:10.3390/genes9040210

Cited by 38 publications

(24 citation statements)

References 73 publications

(124 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The actual physiological significance of AHL-degrading enzymes remains largely unclear 59 . Several authors have suggested that AHL degradation could be related with a self-regulation of intercellular systems 25 . Using different AHL biosensor strains we found that strain P6 does not produce any AHLs, nor could we identify any gene coding for a QS signal synthase (luxI) in the genome of the type strain P. segetis FR1439 T .…”

Section: Discussionmentioning

confidence: 99%

Plant growth-promoting activity and quorum quenching-mediated biocontrol of bacterial phytopathogens by Pseudomonas segetis strain P6

Rodríguez

Torres

Blanco

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Given the major threat of phytopathogenic bacteria to food production and ecosystem stability worldwide, novel alternatives to conventional chemicals-based agricultural practices are needed to combat these bacteria. the objective of this study is to evaluate the ability of Pseudomonas segetis strain P6, which was isolated from the Salicornia europaea rhizosphere, to act as a potential biocontrol agent given its plant growth-promoting (pGp) and quorum quenching (QQ) activities. Seed biopriming and in vivo assays of tomato plants inoculated with strain P6 resulted in an increase in seedling height and weight. We detected QQ activity, involving enzymatic degradation of signal molecules in quorum sensing communication systems, against a broad range of N-acylhomoserine lactones (AHLs). HpLc-MRM data and phylogenetic analysis indicated that the QQ enzyme was an acylase. the QQ activity of strain P6 reduced soft rot symptoms caused by Dickeya solani, Pectobacterium atrosepticum and P. carotovorum on potato and carrot. In vivo assays showed that the PGP and QQ activities of strain P6 protect tomato plants against Pseudomonas syringae pv. tomato, indicating that strain P6 could have biotechnological applications. To our knowledge, this is the first report to show PGP and QQ activities in an indigenous Pseudomonas strain from Salicornia plants. Plant bacterial pathogens cause diseases in a wide range of crops worldwide and considerable economic losses in agriculture 1,2. Antibiotics and chemical pesticides have been used for many decades to combat plant bacterial infections 3,4. However, stricter legislation has been introduced in recent years regarding the use of chemical-based treatments which have caused serious problems such as reduced productivity due to resistance to treatment, soil salinization and environmental pollution 5. As a consequence, alternative strategies to combat plant diseases and to promote plant growth are required in order to replace current procedures with more sustainable eco-friendly approaches 6. Currently, one of the most promising tools used in the agricultural industry is the use of formulations containing plant growth-promoting bacteria (PGPB), also known as plant growth-promoting rhizobacteria (PGPR). These are beneficial microorganisms that act as biofertilizers and can fight plant pathogens 7,8. They counteract pathogens through physical displacement, siderophore production, as well as the synthesis of antibiotics, bacteriocins and hydrolytic enzymes which inhibit pathogen growth 8,9. They also boost plant resistance to infections, which is also called induced systemic resistance (ISR) 10 , through mechanisms such as callose deposition 11. PGPB, which promote processes such as plant growth and stress tolerance, and can fight phytopathogens, are considered to be an effective, sustainable and environmentally-friendly alternative to be used in agriculture 8,12. Although several members of the genus Pseudomonas have been identified as PGPB, only a few have been isolated from

show abstract

Section: Discussionmentioning

confidence: 99%

Plant growth-promoting activity and quorum quenching-mediated biocontrol of bacterial phytopathogens by Pseudomonas segetis strain P6

Rodríguez

Torres

Blanco

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…A. tumefaciens is a niche‐constructing pathogen that genetically modifies the plant host genome by transferring a part (the transfer DNA (T‐DNA)) of its virulence Ti plasmid (Barton et al ., ; Dessaux & Faure, ). When expressed into the plant cell nucleus, the T‐DNA genes divert the host hormonal and metabolic pathways to provoke the development of galls or plant tumors (Deeken et al ., ).…”

Section: Introductionmentioning

confidence: 99%

The biotroph Agrobacterium tumefaciens thrives in tumors by exploiting a wide spectrum of plant host metabolites

et al. 2019

Self Cite

View full text Add to dashboard Cite

Agrobacterium tumefaciens is a niche-constructing biotroph that exploits host plant metabolites.We combined metabolomics, transposon-sequencing (Tn-seq), transcriptomics, and reverse genetics to characterize A. tumefaciens pathways involved in the exploitation of resources from the Solanum lycopersicum host plant.Metabolomics of healthy stems and plant tumors revealed the common (e.g. sucrose, glutamate) and enriched (e.g. opines, c-aminobutyric acid (GABA), c-hydroxybutyric acid (GHB), pyruvate) metabolites that A. tumefaciens could use as nutrients. Tn-seq and transcriptomics pinpointed the genes that are crucial and/or upregulated when the pathogen grew on either sucrose (pgi, kdgA, pycA, cisY) or GHB (blcAB, pckA, eno, gpsA) as a carbon source. While sucrose assimilation involved the Entner-Doudoroff and tricarboxylic acid (TCA) pathways, GHB degradation required the blc genes, TCA cycle, and gluconeogenesis. The tumorenriched metabolite pyruvate is at the node connecting these pathways. Using reverse genetics, we showed that the blc, pckA, and pycA loci were important for aggressiveness (tumor weight), proliferation (bacterial charge), and/or fitness (competition between the constructed mutants and wild-type) of A. tumefaciens in plant tumors.This work highlighted how a biotroph mobilizes its central metabolism for exploiting a wide diversity of resources in a plant host. It further shows the complementarity of functional genome-wide scans by transcriptomics and Tn-seq to decipher the lifestyle of a plant pathogen.

show abstract

“…Proline was recently proposed to modulate the plant defense response to Agrobacterium tumefaciens. Proline accumulates in plant tumors, and functions as a competitive antagonist of gamma-aminobutyric (GABA)-dependent plant defense, interfering with the GABA-induced degradation of quorum-sensing signal [44][45][46]. Proline can therefore promote Agrobacterium infection and horizontal transfer of the Ti plasmid.…”

Section: Regulatory Functions Of Prolinementioning

confidence: 99%

“…Proline can therefore promote Agrobacterium infection and horizontal transfer of the Ti plasmid. The above-listed examples suggest that proline functions as versatile cellular signal in both animal and plant cells [44,45].…”

Section: Regulatory Functions Of Prolinementioning

confidence: 99%

Proline, A Peculiar Amino Acid with Astucious Functions in Development and Salt Tolerance Process in Plants

Saibi¹,

Brini²

2020

JFNM

View full text Add to dashboard Cite

Proline is known to play diverse functions in plants. Some aspects of its biological functions are still unclear. This review highlights some cases in the proline, structure, metabolism, functions in development and also its involvement in salt tolerance process in planta. Indeed, we report the clever roles of proline in cellular homeostasis, including redox balance and their implication as effector during some causal enzymological and physiological processes. Furthermore, the proline functions under abiotic stresses are not yet completely understood. The engineering of proline metabolism could lead to new opportunities to improve plant tolerance against environmental stresses, especially salinity. Eventually, we note that the purpose through this review is to provide a rich, concise and mostly cohesive source on proline, considered as a platform and an anchor between several disciplines and biological functions. We also provide insight on some important research gaps that need to be filled to advance our scientific understanding in this area of research on proline in soil-plant systems.

show abstract

Quorum Sensing and Quorum Quenching in Agrobacterium: A “Go/No Go System”?

Cited by 38 publications

References 73 publications

Plant growth-promoting activity and quorum quenching-mediated biocontrol of bacterial phytopathogens by Pseudomonas segetis strain P6

Plant growth-promoting activity and quorum quenching-mediated biocontrol of bacterial phytopathogens by Pseudomonas segetis strain P6

The biotroph Agrobacterium tumefaciens thrives in tumors by exploiting a wide spectrum of plant host metabolites

Proline, A Peculiar Amino Acid with Astucious Functions in Development and Salt Tolerance Process in Plants

Contact Info

Product

Resources

About