Quorum sensing-dependent metalloprotease VvpE is important in the virulence of Vibrio vulnificus to invertebrates

Ha, Changwan; Kim, Sang-Yong; Lee, Mi-Nan; Lee, Joon-Hee

doi:10.1016/j.micpath.2014.04.001

Cited by 18 publications

(11 citation statements)

References 28 publications

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“…This may be due to differences in the host system. Actually, when we investigated the role of QS and vvpE using an insect model, the Tenebrio molitor larva (a mealworm) that is a host similar to the brine shrimp, both SmcR and VvpE were crucial in the V. vulnificus virulence to the insect (Ha et al, 2014). Consistently, QS and Protease IV of P. aeruginosa were also crucial for the virulence of P. aeruginosa to the insect, suggesting that QS and proteases may be generally important in the bacterial virulence to small invertebrate animals.…”

Section: Discussionmentioning

confidence: 99%

Bacterial virulence analysis using brine shrimp as an infection model in relation to the importance of quorum sensing and proteases

Lee

Kim

et al. 2014

J. Gen. Appl. Microbiol.

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Brine shrimp are aquatic crustaceans belonging to a genus of Artemia. This organism is widely used for testing the toxicity of chemicals. In this study, brine shrimp were evaluated as an infection model organism to study bacterial virulence. Artemia nauplii were infected with various pathogenic bacteria, such as Vibrio vulnificus, Pseudomonas aeruginosa, Burkholderia vietnamiensis, Staphylococcus aureus, and Escherichia coli, and the susceptibility to these bacteria was investigated by counting the survival of the infected nauplii. While all of the tested bacteria have significant virulence to brine shrimp, killing the nauplii in a few days, V. vulnificus showed the strongest virulence. P. aeruginosa also showed a dose-dependent virulence to brine shrimp, but the virulence was weaker than that of V. vulnificus. The virulence tests using the virulence-attenuated mutants of V. vulnificus and P. aeruginosa, such as quorum sensing (QS) mutants or protease-deficient mutants showed a significant attenuation of virulence, demonstrating that the QS mechanism is important in the virulence of these bacteria to brine shrimp. B. vietnamiensis, S. aureus, and E. coli were also virulent to brine shrimp and the virulence was correlated with dosage within 24 h under our conditions. Salmonella enterica Typhimurium and Bacillus subtilis were also virulent to brine shrimp, but the virulence was weak and slowly exerted compared with that of other bacteria. Taken together, we suggest that brine shrimp are a good infection model to assay bacterial virulence, especially for V. vulnificus and P. aeruginosa, and QS is important in the bacterial virulence to brine shrimp.Key words: brine shrimp; infection model; protease; Pseudomonas aeruginosa; quorum sensing; Vibrio vulnificus IntroductionThe brine shrimp is an aquatic crustacean that is frequently used as a standard organism for testing the toxicity of chemicals and as live food in the larviculture of economically important fishes and crustaceans (Sorgeloos et al., 1978;Peroone and Wells, 1987;Orozco-Medina et al., 2002;Marques et al., 2006). A genus of brine shrimp, Artemia, is known to exist worldwide in inland saltwater lakes and is able to live in waters of high salinity (up to 25%), which feature makes the brine shrimp a good host model organism for the virulence study of marine pathogenic bacteria (Marques et al., 2006;Gajardo and Beardmore, 2012). Moreover, Artemia produces dormant eggs, known as cysts, that are stably stored for long periods and hatched as needed (Gajardo and Beardmore, 2012). This characteristic also provides a convenient way to use this organism for scientific applications, as well as for practical uses. Brine shrimp may have additional advantages like their short life-span and good resilience when used as a model organism (Sorgeloos et al., 1978;Peroone and Wells, 1987;Marques et al., 2006;Gajardo and Beardmore, 2012).In this study, we intended to use brine shrimp for testing the virulence of pathogenic bacteria, instead of toxic chemicals. Currently, there...

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Section: Discussionmentioning

confidence: 99%

Bacterial virulence analysis using brine shrimp as an infection model in relation to the importance of quorum sensing and proteases

Lee

Kim

et al. 2014

J. Gen. Appl. Microbiol.

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“…After colonization, bacterial population density increases, AIs accumulate in the microenvironment, and bacterial cells detect these messages to track changes in population size as a mechanism to modulate gene expression [72]. QS has been shown to control bacterial bioluminescence [73], biofilm formation [74], toxin secretion [75], motility [76], sporulation [77], and virulence factor expression [78]. This unique bacterial communication makes anti-QS compounds a promising way to selectively counter bacterial virulence, making the interference of QS and AIs production putative targets for alternative therapies.…”

Section: Anti-quorum Sensing Systemmentioning

confidence: 99%

Defeating Antibiotic-Resistant Bacteria: Exploring Alternative Therapies for a Post-Antibiotic Era

Wang

Hsieh

Powers

et al. 2020

IJMS

123

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Antibiotics are one of the greatest medical advances of the 20th century, however, they are quickly becoming useless due to antibiotic resistance that has been augmented by poor antibiotic stewardship and a void in novel antibiotic discovery. Few novel classes of antibiotics have been discovered since 1960, and the pipeline of antibiotics under development is limited. We therefore are heading for a post-antibiotic era in which common infections become untreatable and once again deadly. There is thus an emergent need for both novel classes of antibiotics and novel approaches to treatment, including the repurposing of existing drugs or preclinical compounds and expanded implementation of combination therapies. In this review, we highlight to utilize alternative drug targets/therapies such as combinational therapy, anti-regulator, anti-signal transduction, anti-virulence, anti-toxin, engineered bacteriophages, and microbiome, to defeat antibiotic-resistant bacteria.

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“…A bactéria produz vários fatores de virulência incluindo polissacarídeos capsulares (CPS) e extracelulares, hemolisina, dentre outros, que atuam primariamente para auxiliar na sua sobrevivência às adversidades ambientais e podem ter ação patogênica (Jones & Oliver, 2009). Ha et al (2014) sugerem que os genes de virulência em V. vulnificus podem ser ativados pelo sistema de regulação de densidade QS e que o sistema imune dos diferentes invertebrados pode responder de diversas maneiras ao agentes patogênicos.…”

Section: Vibrio Vulnificusunclassified

Vibrio spp . COMO PATÓGENOS NA CARCINICULTURA: ALTERNATIVAS DE CONTROLE

Rebouças

Menezes

Vieira

et al. 2017

ACMAR

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RESUMOA carcinicultura é uma atividade importante da agroindústria em várias partes do mundo e que sofre perdas significativas devido à ocorrência de doenças. Dentre os agentes patogênicos, as bactérias do gênero Vibrio têm destaque pelos elevados índices de mortalidade registrados. O emprego de antibióticos é a opção mais frequente no combate a essas enfermidades, entretanto seu uso não criterioso aumenta a pressão seletiva sobre estirpes bacterianas resistentes no ambiente aquático, o que resulta em risco ambiental e para a saúde de animais e humanos. Novas tecnologias vêm sendo desenvolvidas no combate de doenças nos ambientes de cultivo de organismos aquáticos prevenindo assim, perdas econômicas. Esforços vêm sendo aplicados para a compreensão dos processos infecciosos e rotas de invasão das estirpes de Vibrio em camarões cultivados a fim de estabelecer melhores técnicas de combate aos patógenos. Procedimentos de biossegurança nos cultivos devem ser desenvolvidos integrando conceitos de saúde, nutrição, genética e qualidade ambiental para um efetivo controle e combate de enfermidades. Este trabalho de revisão tem como objetivo traçar um perfil sobre esse novo panorama que se desenha na atividade da carcinicultura e as alternativas no combate às vibrioses.Palavras-chave: biossegurança, carcinicultura, vibriose, patógeno. ABSTRACTShrimp farming, an important agribusiness activity worldwide, experiences significant losses due to diseases. Among the pathogenic agents, bacteria of the Vibrio genus are prominent for the high mortality rate registered in infections. Antibiotics are the most frequent option in to fight such

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Quorum sensing-dependent metalloprotease VvpE is important in the virulence of Vibrio vulnificus to invertebrates

Cited by 18 publications

References 28 publications

Bacterial virulence analysis using brine shrimp as an infection model in relation to the importance of quorum sensing and proteases

Bacterial virulence analysis using brine shrimp as an infection model in relation to the importance of quorum sensing and proteases

Defeating Antibiotic-Resistant Bacteria: Exploring Alternative Therapies for a Post-Antibiotic Era

Vibrio spp . COMO PATÓGENOS NA CARCINICULTURA: ALTERNATIVAS DE CONTROLE

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