Quorum sensing inhibitory effect of bergamot oil and aspidosperma extract against Chromobacterium violaceum and Pseudomonas aeruginosa

Ahmed, Sarah Omar; Zedan, Hamdallah; Ibrahim, Yasser Musa

doi:10.1007/s00203-021-02455-8

Cited by 14 publications

(7 citation statements)

References 52 publications

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“…The motility of S. putrefaciens was significantly ( p < 0.05) affected by the OGEO in a concentration-dependent manner. Swimming motility is a flagellum-dependent movement of individual cells observed in semi-solid media [ 36 ]. As shown in Table 3 , high concentrations of OGEO impaired the motility of S. putrefaciens .…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Effect of Ocimum gratissimum L. Essential Oil on Shewanella putrefaciens: Insights Based on the Cell Membrane and External Structure

Xie

Zhang

Mei

et al. 2023

IJMS

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The main objective of this study was to assess the in vitro antibacterial effectiveness of Ocimum gratissimum L. essential oil (OGEO) against Shewanella putrefaciens. The minimum inhibitory concentration and minimum bactericidal concentration of OGEO acting on S. putrefaciens were both 0.1% and OGEO could inhibit the growth of S. putrefaciens in a dose-dependent manner. The restraint of the biofilm growth of S. putrefaciens was found in the crystal violet attachment assay and confocal laser scanning microscopy. The disruption of cell membranes and exudation of contents in S. putrefaciens with OGEO treatment were observed by scanning electron microscopy, hemolysis and ATPase activity. The results demonstrated that OGEO had a positive inhibitory effect on the growth of S. putrefaciens, which primarily developed its antibacterial function against S. putrefaciens by disrupting the formation of biofilms and cell membranes. This study could provide a new method of inhibiting the spoilage of food in which the dominant spoilage bacteria are S. putrefaciens.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial Effect of Ocimum gratissimum L. Essential Oil on Shewanella putrefaciens: Insights Based on the Cell Membrane and External Structure

Xie

Zhang

Mei

et al. 2023

IJMS

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“…Among the plant essential oils, immortelle (Helichrysum italicum), Origanum majorana, thyme and citrus showed valuable biofilm inhibition activity against P. aeruginosa and H. influenzae [51][52][53][54].…”

Section: Biofilm Inhibitorsmentioning

confidence: 99%

“…Dioon spinulosum extract P. aeruginosa [49] Plant-derived triterpenes (analogs of oleanolic acid) P. aeruginosa, B. cenocepacia [50] Plant essential oils P. aeruginosa, H. influenzae [51][52][53][54] Ceragenins (CSAs) A. xylosoxidans [55] Metalloproteases from stony coral S. maltophilia [56] Vitamin C S. maltophilia [57] Allium stipitatum extract S. maltophilia [58] Glycosyl hydrolases PslG from P. fluorescens Pseudomonas strains…”

Section: Biofilm Inhibitors Bacteria Referencementioning

confidence: 99%

New Antimicrobial Strategies to Treat Multi-Drug Resistant Infections Caused by Gram-Negatives in Cystic Fibrosis

Scoffone,

Barbieri,

Irudal

et al. 2024

Antibiotics

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People with cystic fibrosis (CF) suffer from recurrent bacterial infections which induce inflammation, lung tissue damage and failure of the respiratory system. Prolonged exposure to combinatorial antibiotic therapies triggers the appearance of multi-drug resistant (MDR) bacteria. The development of alternative antimicrobial strategies may provide a way to mitigate antimicrobial resistance. Here we discuss different alternative approaches to the use of classic antibiotics: anti-virulence and anti-biofilm compounds which exert a low selective pressure; phage therapies that represent an alternative strategy with a high therapeutic potential; new methods helping antibiotics activity such as adjuvants; and antimicrobial peptides and nanoparticle formulations. Their mechanisms and in vitro and in vivo efficacy are described, in order to figure out a complete landscape of new alternative approaches to fight MDR Gram-negative CF pathogens.

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“…Agar well diffusion assay was used to detect violacein inhibition activity of the plant extract adopting standard method described by Ahmed et al 41 . Overnight cultures of C. violaceum (MTCC2656) in LB broth were diluted to 0.5 McFarland.…”

Section: Violacein Inhibition Assay Using C Violaceum (Mtcc2656)mentioning

confidence: 99%

Evaluation of Biofilm Inhibitory Efficacy of Bacopa monnieri, Acalypha indica and Calotropis gigantea Extracts and their Combination Against Wound Colonizing Bacteria Pseudomonas aeruginosa and Staphylococcus Aureus

Tanimu

Vijayan

Sukumaran

2022

Int J Life Sci Pharm Res

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Key factors in compromised wound healing primarily include bacterial colonization and infection. Extensive use of systemic antibiotics, preferred choice of treatment for clinically infected wounds, is often accompanied with emergence of bacterial resistance. Quorum sensing (QS), density dependent chemical communication, is emerging as a promising area of research since most infectious microorganisms operate this mechanism to realize their pathogenic potential. Foregoing research indicates the potential of studying medicinal plant extracts/components and their combinations as vital inhibitors of QS regulated virulence factors’ production. Our study aims to understand the potential of three plant extracts and their combinations as vital inhibitors of QS regulated virulence factor’s production by two wound pathogens and to achieve this our objectives are to perform their violacein inhibitory (on C. violaceum) and biofilm inhibitory properties (on Pseudomonas aeruginosa and Staphylococcus aureus). Minimum inhibitory concentration (MIC) of aqueous plant extracts against P aeruginosa and S aureus was performed (two-fold serial microdilution). Violacein inhibition was assayed using agar well diffusion while biofilm inhibition by crystal violet method. Organisms presented 5mg/ml (Bacopa monnieri ), 2.5mg/ml (Acalypha indica), and 2.5mg/ml (Calotropis gigantea) as MIC. Three sets of individual extract concentrations (sub-MIC in μg/ml) and their corresponding combinations were used for this study. Extracts exhibited more significant (P<0.0001) violacein inhibition in combination than individually. Biofilm inhibition by extracts' combination was also significantly (P<0.001) higher than that of individual extracts indicating a possible positive herb-herb interaction of phytoconstituents (synergistic or complementary). The relative decrease in response to individual extracts in higher concentrations (set 'c') by both organisms point to possible recalcitrance behavior generally exhibited by bacteria on exposure to higher antibacterial agents. The improved antibiofilm efficacy exhibited by this novel combination may serve as an alternative approach in managing wound colonization by biofilm-producing bacteria and hence faster-wound healing.

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Quorum sensing inhibitory effect of bergamot oil and aspidosperma extract against Chromobacterium violaceum and Pseudomonas aeruginosa

Cited by 14 publications

References 52 publications

Antimicrobial Effect of Ocimum gratissimum L. Essential Oil on Shewanella putrefaciens: Insights Based on the Cell Membrane and External Structure

Antimicrobial Effect of Ocimum gratissimum L. Essential Oil on Shewanella putrefaciens: Insights Based on the Cell Membrane and External Structure

New Antimicrobial Strategies to Treat Multi-Drug Resistant Infections Caused by Gram-Negatives in Cystic Fibrosis

Evaluation of Biofilm Inhibitory Efficacy of Bacopa monnieri, Acalypha indica and Calotropis gigantea Extracts and their Combination Against Wound Colonizing Bacteria Pseudomonas aeruginosa and Staphylococcus Aureus

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