Quorum-Sensing Regulation of Adhesion in
            <i>Serratia marcescens</i>
            MG1 Is Surface Dependent

Labbate, Maurizio; Zhu, Hua; Thung, Leena; Bandara, Rani; Larsen, Martin R.; Willcox, Mark; Givskov, Michael; Rice, Scott A.; Kjelleberg, Staffan

doi:10.1128/jb.01582-06

Cited by 98 publications

(94 citation statements)

References 60 publications

(54 reference statements)

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“…The same study found a role for certain genes, including bsmA, bsmB, and the type I fimbrial adhesin gene fimA, in the attachment of S. marcescens MG1 to immortalized corneal epithelial cells (31). Other studies have revealed the importance of quorum sensing regulation in biofilm maturation and biofilm-dependent protection against predation by protozoans (30,50,51).…”

mentioning

confidence: 82%

“…Surface proteins from cells grown overnight on LB plates at 30°C were prepared as previously described (31). Trichloroacetic acid-precipitated proteins were washed twice with cold acetone, dried, resuspended in 10 l of TE and 15 l of polyacrylamide gel electrophoresis (PAGE) sample buffer, boiled, and run on an 8 to 16% gradient gel.…”

Section: Methodsmentioning

confidence: 99%

“…These large surface structures also play a key role in attachment to epithelial cells and pathogenesis (11,31,40). These appendages are also important in pellicle formation and impart growth advantages in some environmental niches (16,42).…”

mentioning

confidence: 99%

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ASerratia marcescensOxyR Homolog Mediates Surface Attachment and Biofilm Formation

Shanks

Stella²,

Kalivoda³

et al. 2007

J Bacteriol

104

131

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OxyR is a conserved bacterial transcription factor with a regulatory role in oxidative stress response. From a genetic screen for genes that modulate biofilm formation in the opportunistic pathogen Serratia marcescens, mutations in an oxyR homolog and predicted fimbria structural genes were identified. S. marcescens oxyR mutants were severely impaired in biofilm formation, in contrast to the hyperbiofilm phenotype exhibited by oxyR mutants of Escherichia coli and Burkholderia pseudomallei. Further analysis revealed that OxyR plays a role in the primary attachment of cells to a surface. Similar to what is observed in other bacterial species, S. marcescens OxyR is required for oxidative stress resistance. Mutations in oxyR and type I fimbrial genes resulted in severe defects in fimbria-associated phenotypes, revealing roles in cell-cell and cell-biotic surface interactions. Transmission electron microscopy revealed the absence of fimbria-like surface structures on an OxyR-deficient strain and an enhanced fimbrial phenotype in strains bearing oxyR on a multicopy plasmid. The hyperfimbriated phenotype conferred by the multicopy oxyR plasmid was absent in a type I fimbrial mutant background. Real-time reverse transcriptase PCR indicated an absence of transcripts from a fimbrial operon in an oxyR mutant that were present in the wild type and a complemented oxyR mutant strain. Lastly, chromosomal P lac -mediated expression of fimABCD was sufficient to restore wild-type levels of yeast agglutination and biofilm formation to an oxyR mutant. Together, these data support a model in which OxyR contributes to early stages of S. marcescens biofilm formation by influencing fimbrial gene expression.

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confidence: 82%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

ASerratia marcescensOxyR Homolog Mediates Surface Attachment and Biofilm Formation

Shanks

Stella²,

Kalivoda³

et al. 2007

J Bacteriol

104

131

View full text Add to dashboard Cite

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“…These adhesins probably interact with mannose glycoproteins expressed on the surface of corneal ep ithelial cells as a result of abrasion or trauma due to the use of contact lenses. [98]; identified type I fimbriae as the critical adhesin that mediate attachment of S. marcescens to human corneal epithelial cell surface. They also identified two AHL (N-acylhomoserine lactone)-regulated genes, bsmA and bsmB, to be involved in adhesion to biotic surface and the expression of these genes leads to the attachment of S. marcescens to HCE cells.…”

Section: Adhesionmentioning

confidence: 99%

Pathogenesis of microbial keratitis

Lakhundi

Siddiqui

Khan

2017

Microbial Pathogenesis

181

121

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Microbial keratitis is a sight-threatening ocular infection caused by bacteria, fungi, and protist pathogens. Epithelial defects and injuries are key predisposing factors making the eye susceptible to corneal pathogens. Among bacterial pathogens, the most common agents responsible for keratitis include Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pneumonia and Serratia species. Fungal agents of corneal infections include both filamentous as well as yeast, including Fusarium, Aspergillus, Phaeohyphomycetes, Curvularia, Paecilomyces, Scedosporium and Candida species, while in protists, Acanthamoeba spp. are responsible for causing ocular disease. Clinical features include redness, pain, tearing, blur vision and inflammation but symptoms vary depending on the causative agent. The underlying molecular mechanisms associated with microbial pathogenesis include virulence factors as well as the host factors that aid in the progression of keratitis, resulting in damage to the ocular tissue. The treatment therefore should focus not only on the elimination of the culprit but also on the neutralization of virulence factors to minimize the damage, in addition to repairing the damaged tissue. A complete understanding of the pathogenesis of microbial keratitis will lead to the rational development of therapeutic interventions. This is a timely review of our current understanding of the advances made in this field in a comprehensible manner. Coupled with the recently available genome sequence information and high throughput genomics technology, and the availability of innovative approaches, this will stimulate interest in this field.

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“…Quorum sensing is also required for normal attachment and biofilm formation by the aquatic bacterium V. vulnificus (101). Interestingly, in some species, such as Serratia marcescens, the requirement for quorum-sensing regulation of attachment depends on the nature of the surface, with adherence to abiotic but not biotic surfaces being modulated by quorum sensing (87). Overall, the importance of quorum sensing in the regulation of cell-surface and cell-cell attachment has been repeatedly reported for a variety of other bacterial species, including E. coli, L. monocytogenes, Burkholderia cenocepacia, and Burkholderia cepacia (22,37,52,64,154).…”

Section: Figmentioning

confidence: 99%