Quorum sensing signals and related virulence inhibition of Pseudomonas aeruginosa by a potential probiotic strain's organic acid

Kiymaci, Merve Eylul; Altanlar, Nurten; Gümüştaş, Mehmet; Özkan, Síbel A.; Akın, Alper

doi:10.1016/j.micpath.2018.05.042

Cited by 64 publications

(48 citation statements)

References 36 publications

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“…Therefore, some authors found out that the probiotic L. fermentum has the ability to attenuate the biofilm-forming potential of P. aeruginosa PAO1 due to postbiotics: bacteriocins and exopolysaccharides (Sharma et al, 2018). Other authors have shown that lactic acid has an inhibitory effect on biofilm formation by P. aeruginosa through QS system (Kiymaci et al, 2018). De Marco et al examined the ability of E. coli to form biofilm in the absence or presence of supernatants of several probiotic microorganisms: L. acidophilus, L. lactis, L. casei, L. reuteri and Saccharomyces boulardii.…”

Section: Discussionmentioning

confidence: 99%

Influence of cell-free extracts of Bifidobacterium bifidum and Lactobacillus reuteri on proliferation and biofilm formation by Escherichia coli and Pseudomonas aeruginosa

Knysh

Isayenko

Voyda

et al. 2019

Regul. Mech. Biosyst.

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The development of new effective preparations for the correction of microecological disorders based on probiotic derivatives requires a comprehensive study of the biological activity of the latter. We studied the proliferative activity and biofilm formation by clinical isolates: Escherichia coli and Pseudomonas aeruginosa under the influence of cell-free extracts containing structural components and metabolites of the Bifidobacterium bifidum and Lactobacillus reuteri probiotic strains. Cell-free extracts were obtained from disintegrates and cultures of probiotics. Disintegrates were prepared by cyclic freezing-thawing of probiotic cell suspensions. The cultures were obtained by cultivating probiotic microorganisms in their own disintegrates. The obtained disintegrates and cultures were filtered. The proliferative activity of the test cultures was studied using the spectrophotometric microtiter plate method after an hour-long exposure in undiluted cell-free extracts and subsequent cultivation in a nutrient medium containing 30%vol of the studied extracts at 37 °C for 24 hours. The biofilm formation of the test cultures was studied with 30% vol content of cell-free extracts in the cultivation medium using the spectrophotometric microtiter plate method. All the studied extracts exerted a similar effect on the proliferative activity and biofilm formation by E. coli and P. aeruginosa. Exposure of the test cultures in all undiluted extracts during an hour led to a significant decrease in the optical density of the test samples: optical density of the test wells ranged from 36.5% to 49.8% of the control wells. The test cultures that were exposed to the extracts: filtrate of L. reuteri disintegrate (L), filtrate of В. bifidum disintegrate (B) and filtrate of В. bifidum culture, grown in В. bifidum disintegrate (MB) after dilution and subsequent cultivation over the next 24 hours completely restored the ability to proliferate. The proliferative activity of the test cultures that were exposed to the extracts: filtrate of L. reuteri culture, grown in L. reuteri disintegrate (ML) and filtrate of L. reuteri culture, grown in L. reuteri disintegrate supplemented with 0.8 M glycerol and 0.4 M glucose (MLG), was significantly inhibited after dilution and subsequent cultivation. The inhibition indices calculated for the ML extract were: 25.9% (E. coli) and 53.0% (P. aeruginosa). Inhibition indices calculated for the MLG extract were: 62.0% (E. coli) and 96.9% (P. aeruginosa). MLG extract had more pronounced inhibitory effect on the proliferation of the test cultures than ML extract. All the studied extracts exerted significant inhibitory effect on the biofilm formation of the test cultures. Analysis of the results of the study shows that cell-free extracts of L. reuteri culture grown in its disintegrate without supplementation or supplemented with glycerol and glucose have the highest antimicrobial activity and can be used as metabiotics to prevent overgrowth of potentially pathogenic bacteria, as well as inoculation and proliferation of pathogenic gram-negative bacteria in the gastrointestinal tract. They can be used alone or in combination with cellular probiotics to enhance their probiotic action. This study encourages further careful investigation of the biochemical composition of cell-free extracts and clarifying the mechanism of their action.

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Section: Discussionmentioning

confidence: 99%

Influence of cell-free extracts of Bifidobacterium bifidum and Lactobacillus reuteri on proliferation and biofilm formation by Escherichia coli and Pseudomonas aeruginosa

Knysh

Isayenko

Voyda

et al. 2019

Regul. Mech. Biosyst.

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“…Some probiotics have also inhibitory effects on QS systems that inhibit the QS-dependent physiologic behaviors of bacteria. 44 Lactic acid produced by probiotics had shown an inhibitory effect on QS by suppressing short-chain AHL production and biofilm formation of P. aeruginosa that is regulated by QS. 44 Probiotics also secret organic acid as QS antagonists that interfere with AHLs production at the gene expression level and prevent biofilm formation.…”

Section: Probiotics Influence Gene Expression Of Pathogenic Biofilmsmentioning

confidence: 99%

“…44 Lactic acid produced by probiotics had shown an inhibitory effect on QS by suppressing short-chain AHL production and biofilm formation of P. aeruginosa that is regulated by QS. 44 Probiotics also secret organic acid as QS antagonists that interfere with AHLs production at the gene expression level and prevent biofilm formation. 45 Biosurfactants isolated from L. plantarum and Pediococcus acidilactici could inhibit the adhesion and biofilm formation of S.aureus CMCC 26003 in a dosedependent manner in vitro.…”

Section: Probiotics Influence Gene Expression Of Pathogenic Biofilmsmentioning

confidence: 99%

<p>The Battle of Probiotics and Their Derivatives Against Biofilms</p>

Barzegari¹,

Kheyrolahzadeh²,

Khatibi³

et al. 2020

IDR

175

112

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Biofilm-related infections have been a major clinical problem and include chronic infections, device-related infections and malfunction of medical devices. Since biofilms are not fully available for the human immune system and antibiotics, they are difficult to eradicate and control; therefore, imposing a global threat to human health. There have been avenues to tackle biofilms largely based on the disruption of their adhesion and maturation. Nowadays, the use of probiotics and their derivatives has gained a growing interest in battling against pathogenic biofilms. In the present review, we have a close look at probiotics with the ultimate objective of inhibiting biofilm formation and maturation. Overall, insights into the mechanisms by which probiotics and their derivatives can be used in the management of biofilm infections would be warranted.

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“…Lactic acid produced by a probiotic Pediococcus strain inhibited at sub‐MIC concentrations the production of short‐chain AHLs as well as swarming and swimming motility of clinical isolates of P. aeruginosa . However, there was no evidence that short‐chain AHL inhibition was causal for inhibiting motility . Various microorganisms share the ability to oxidize bicyclic aromatic compounds like naphthalene and indole.…”

Section: Interspecies Competition and The Microbiotamentioning

confidence: 99%

“…However, there was no evidence that short-chain AHL inhibition was causalf or inhibiting motility. [155] Various microorganisms share the ability to oxidize bicyclic aromatic compounds like naphthalenea nd indole. The oxidation products 1-naphtol as well as different hydroxyindoles completely blocked swarming motility of P. aeruginosa at 50 mm.T he activity wasf ound not to be related to changes in c-di-GMP levels or rhamnolipid pro- ductiona nd was restricted to inhibition of swarming but not swimming motility.…”

Section: Interspecies Competition and The Microbiotamentioning

confidence: 99%

Inhibitors of Bacterial Swarming Behavior

Rütschlin

Böttcher

2019

Chemistry A European J

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Bacteria can migrate in groups of flagella‐driven cells over semisolid surfaces. This coordinated form of motility is called swarming behavior. Swarming is associated with enhanced virulence and antibiotic resistance of various human pathogens and may be considered as favorable adaptation to the diverse challenges that microbes face in rapidly changing environments. Consequently, the differentiation of motile swarmer cells is tightly regulated and involves multi‐layered signaling networks. Controlling swarming behavior is of major interest for the development of novel anti‐infective strategies. In addition, compounds that block swarming represent important tools for more detailed insights into the molecular mechanisms of the coordination of bacterial population behavior. Over the past decades, there has been major progress in the discovery of small‐molecule modulators and mechanisms that allow selective inhibition of swarming behavior. Herein, an overview of the achievements in the field and future directions and challenges will be presented.

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Quorum sensing signals and related virulence inhibition of Pseudomonas aeruginosa by a potential probiotic strain's organic acid

Cited by 64 publications

References 36 publications

Influence of cell-free extracts of Bifidobacterium bifidum and Lactobacillus reuteri on proliferation and biofilm formation by Escherichia coli and Pseudomonas aeruginosa

Influence of cell-free extracts of Bifidobacterium bifidum and Lactobacillus reuteri on proliferation and biofilm formation by Escherichia coli and Pseudomonas aeruginosa

<p>The Battle of Probiotics and Their Derivatives Against Biofilms</p>

Inhibitors of Bacterial Swarming Behavior

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