Quorum sensing systems of regulation, synthesis of phenazine antibiotics, and antifungal activity in rhizospheric bacterium pseudomonas chlororaphis 449

Veselova, M. A.; Klein, Sh.; Bass, I. A.; Липасова, В. А.; Metlitskaya, Anastasia; Ovadis, Marianna; Chernin, Leonid; Khmel, I. A.

doi:10.1134/s102279540812003x

Cited by 30 publications

(28 citation statements)

References 24 publications

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“…As previously shown (Veselova et al 2008), P. chlororaphis strain 449 produces at least 3 types of AHLs: N-butanoyl-HSL, N-hexanoyl-HSL and N-(3-oxohexanoyl)-HSL. The genes of QS systems PhzI-PhzR and CsaI-CsaR were identified in strain 449.…”

Section: Resultsmentioning

confidence: 85%

“…This strain produces three types of PHZs, viz. PCA, 2-OH-PCA and 2-OH-PHZ, producing also EPRs and lipases (Veselova et al 2008 Pectin substances are essential components of plant cell. As strain 449 inhabiting the rhizosphere could utilize substances of vegetable origin for its nutrition including products of pectin degradation, it was of interest to determine if it produces PG.…”

Section: Resultsmentioning

confidence: 99%

“…The cultures were acidified with CF 3 COOH (pH 2.0) and then extracted 2 × with AcOEt (Delaney et al 2001). Absorption maxima for PHZs were measured at 250-257 and 366-369 nm (Veselova et al 2008) on a spectrophotometer UV2100 (Shimadzu). DNA manipulations.…”

Section: Methodsmentioning

confidence: 99%

“…We have shown earlier that PHZs were responsible for the in vitro antifungal activity of this strain as well as for its ability to protect plants against soil-born and foliage diseases caused by Rhizoctonia solani and Sclerotinia sclerotiorum, respectively. Strain 449 possessed at least two quorum sensing systems (PhzI-PhzR and CsaICsaR) and produced at least three types of AHL (Klein et al 2004;Veselova et al 2003Veselova et al , 2008.…”

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confidence: 98%

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GacS-dependent regulation of enzymic and antifungal activities and synthesis of N-acylhomoserine lactones in rhizospheric strain Pseudomonas chlororaphis 449

et al. 2009

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Pseudomonas chlororaphis strain 449 isolated from the rhizosphere of maize suppresses numerous plant pathogens in vitro. The strain produces phenazine antibiotics and synthesizes at least three types of quorum sensing signaling molecules, N-acylhomoserine lactones. Here we have shown that the rhizospheric P. chlororaphis strains 449, well known strain 30-84 as well as two other P. chlororaphis strains exhibit polygalacturonase activity. Using mini-Tn5 transposon mutagenesis, four independent mutants of strain P. chlororaphis 449 with insertion of mini-Tn5 Km2 in gene gacS of two-component GacA-GacS system of global regulation were selected. All these mutant strains were deficient in production of extracellular proteinase(s), phenazines, N-acylhomoserine lactones synthesis, and did not inhibit the growth of G(+) bacteria in comparison with the wild type strain. The P. chlororaphis 449-06 gacS (-) mutant studied in greater detail was deficient in polygalacturonase, pectin methylesterase activities, swarming motility and antifungal activity. It is the first time the involvement of GacA-GacS system in the regulation of enzymes of pectin metabolism, polygalacturonase and pectin methylesterase, was demonstrated in fluorescent pseudomonads.

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Section: Resultsmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 98%

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GacS-dependent regulation of enzymic and antifungal activities and synthesis of N-acylhomoserine lactones in rhizospheric strain Pseudomonas chlororaphis 449

et al. 2009

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“…QS system, (CsaI-CsaR), has also been identified in P. aureofaciens which regulates the cell surface properties that confers rhizosphere fitness (Zhang and Pierson 2001). In P. chlororaphis strains GacA/GacS system is operational depending upon regulators PhzI/PhzR and CsaI/CsaR (Veselova et al 2008). …”

Section: Quorum Sensing: Cell Density Dependent Colonizationmentioning

confidence: 99%

Mechanistic insights on plant root colonization by bacterial endophytes: a symbiotic relationship for sustainable agriculture

Jha

Panwar

2018

Environmental Sustainability

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Plant-microbe and soil interactions are one of the oldest muse for multi-disciplinary researchers. Plant growth promoting microorganisms influence the host physiology by secreting regulatory chemical signals in the vicinity of plant roots and play a key role in the enhancement of plant growth and expansion. The present review deals with the in-depth understanding of steps involved in host tissues colonization by bacterial endophytes. The molecular insights of these events, particularly for endophytic bacteria, are poorly documented till date. The endophytic bacteria must coexist with the host plant and capable of colonizing the internal plant tissues without being recognized as a pathogen. A proper understanding of exchange of signals between the host plant and bacterial communities is required which may facilitate the development of new strategies to promote beneficial interactions between them. This knowledge can be instrumental in agricultural practices as well as for phytoremediation of pollutants. Keeping these facts in mind, the present review attempts to explore the systematic understanding of steps involved and molecular insights of plant colonization events by endophytic bacteria. We conclude that molecular mechanisms and factors affecting endophytic bacterial colonization deserve more research attention in order to exploit their beneficial aspects for sustainable agriculture and environment.

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Quorum‐Sensing Quenching by Volatile Organic Compounds Emitted by Rhizosphere Bacteria

Chernin

Toklikishvili

Ovadis

et al. 2013

Molecular Microbial Ecology of the Rhizosphere

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Quorum sensing systems of regulation, synthesis of phenazine antibiotics, and antifungal activity in rhizospheric bacterium pseudomonas chlororaphis 449

Cited by 30 publications

References 24 publications

GacS-dependent regulation of enzymic and antifungal activities and synthesis of N-acylhomoserine lactones in rhizospheric strain Pseudomonas chlororaphis 449

GacS-dependent regulation of enzymic and antifungal activities and synthesis of N-acylhomoserine lactones in rhizospheric strain Pseudomonas chlororaphis 449

Mechanistic insights on plant root colonization by bacterial endophytes: a symbiotic relationship for sustainable agriculture

Quorum‐Sensing Quenching by Volatile Organic Compounds Emitted by Rhizosphere Bacteria

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