R-123. Pregnancy and implantation rates after transfers on day 4 and 5 of fresh and vitrified embryos

Vanderzwalmen, Pierre; Zech, Herbert; Lejeune, Bernard; Prapas, Yannis; Πράπας, Νικόλαος; Nijs, Michelle; Vandamme, B.; Segal-Bertin, G.; Debauche, C.; Roosendaal, E. Van; Chatziparasidou, A.; Stecher, Astrid; Puissant, Françoise; Wissman, D.; Schoysman, R

doi:10.1093/humrep/14.suppl_3.333-b

Cited by 6 publications

(3 citation statements)

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“…Furthermore, ethylene g l y c o l ( E G ) h as b e e n u s e d w i d e l y f o r t h e vitrification of human oocytes [10,25] and embryos [26,27,30,31,[36][37][38] due to its low molecular weight, high permeation ability and low toxicity. Moreover, it has been reported that cryoprotectant mixtures may have some advantages over solutions containing only one permeable cryoprotectant [16].…”

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confidence: 99%

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

Chian

Kuwayama

Tan

et al. 2004

Journal of Reproduction and Development

166

100

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Abstract. Improving pregnancy rates associated with the use of cryopreserved human oocytes would be an important advance in human assisted reproductive technology (ART). Vitrification allows glasslike solidification of a solution without ice crystal formation in the living cells. We have attempted to improve the survival rates of oocytes by a vitrification technique using bovine models. In vitro matured oocytes with or without cumulus cells were vitrified with either 15.0% (v/v) ethylene glycol (EG) + 15% (v/v) dimethylsulfoxide (DMSO) + 0.5 M sucrose or 15% (v/v) EG + 15% (v/v) 1,2-propanediol (PROH) + 0.5 M sucrose, using 'Cryotop' or 'thin plastic sticker', respectively. The oocyte survival rates after vitrifying-warming, and the capacity for fertilization and embryonic development were examined in vitro. The rate of embryonic development to blastocyst was significantly higher (P<0.05) in the oocytes vitrified with 15% (v/v) EG + 15% (v/v) PROH + 0.5 M sucrose than in the oocytes vitrified with 15% (v/v) EG + 15% (v/v) DMSO + 0.5 M sucrose (7.4% ± 4.1 vs. 1.7% ± 3.0, respectively). Oocytes vitrified without cumulus cells had a higher survival rate after thawing and a superior embryonic developmental capacity compared with oocytes vitrified with cumulus cells. Prolonged pre-incubation time after thawing adversely affected the rates of embryonic cleavage and development. These results indicate that in vitro matured bovine oocytes can be vitrified successfully with the mixture of the cryoprotectants, EG + PROH, the absence of cumulus cells for vitrification does not affect oocyte survival rate after warming, and vitrified and warmed oocytes do not require pre-incubation before in vitro fertilization.

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confidence: 99%

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

Chian

Kuwayama

Tan

et al. 2004

Journal of Reproduction and Development

166

100

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“…It has been shown that early blastocysts showed better survival than expanded/hatching blastocysts (Vanderzwalmen et al, 1999;Ebner et al, 2009). Amongst the hypotheses that could explain the differences in the efficacy with which cryopreservation can be performed between different stages of embryo development is the structural difference between morula and blastocyst.…”

Section: Artificial Shrinkage Of Blastocoel Cavitymentioning

confidence: 99%

Micromanipulation Techniques in IVF

Ebner

2010

Journal of Reproductive and Stem Cell Biotechnology

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After the world witnessed groundbreaking early advances in assisted reproduction that utilized conventional in vitro fertilization in the human, more complex invasive techniques of oocyte and embryo manipulation were introduced to treat human subfertility. Amongst these were numerous micromanipulation techniques such as partial zona dissection (PZD; 1988), subzonal insemination (SUZI, 1988) and subsequently ICSI, which became the most powerful tool of assisted fertilization (1992). ICSI requires micromanipulation of both the male and the female gamete. Another important approach is laser-assisted opening of the zona pellucida at different stages of preimplantation development either; to assist hatching (1990) or to remove cells for preimplantation genetic diagnosis (1989), or fragments to restore the integrity of an embryo (1999). Sometimes even blastocysts considered for vitrification need further manipulation in order to artificially shrink the blastocoel which facilitates survival of expanded blastocyst (2002). Other methods for reproductive purposes, however, failed to gain acceptance in population, such as cytoplasm transfer (1997), nuclear transfer (1998) and cloning (1996), to name but a few.

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“…Later in 1999, Vanderzwalmen et al reported the first successful pregnancy using vitrified human blastocysts. However, these initial attempts of blastocyst vitrification using cryostraws have not improved the existing pregnancy rates [7,8]. Later modifications by various workers using different cryoprotectants and better carriers like cryoloop [9], electron microscope grids [10], cryotop [11] enabling faster heat transfer has improved the pregnancy rates.…”

Section: Introductionmentioning

confidence: 99%

Vitrification of human early cavitating and deflated expanded blastocysts: clinical outcome of 474 cycles

Raju¹,

Prakash²,

Krishna³

et al. 2009

J Assist Reprod Genet

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Purpose The present study was undertaken to evaluate and compare the post thaw survival, implantation and pregnancy rates of vitrified human early cavitating blastocysts with deflated expanded blastocysts. Material and methods Supernumerary blastocysts were vitrified in 30% ethylene glycol-dimethyl sulphoxide based solution using cryoloop. Fully expanded blastocysts were deflated by gentle aspiration of the blastocoelic fluid using a micromanipulator until the cavity collapses prior to vitrification. Results Of the 576 vitrified blastocysts, 545 (94.61%) survived thawing in the early cavitating blastocyst group which was significantly higher than deflated expanded blastocyst group, in which only 370 survived thawing out of 459 (80.62%). However, no significant difference was observed in implantation and pregnancy rates between early cavitating and deflated expanded blastocyst groups. Conclusions Early cavitating blastocyst would be the ideal stage for cryopreservation of human blastocysts as it has higher survival rate and avoids additional invasive procedures like deflation of the blastocoele.

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R-123. Pregnancy and implantation rates after transfers on day 4 and 5 of fresh and vitrified embryos

Cited by 6 publications

References 0 publications

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

Micromanipulation Techniques in IVF

Vitrification of human early cavitating and deflated expanded blastocysts: clinical outcome of 474 cycles

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