R-banding pattern of the prometaphase chromosomes of the goat

Berardino, D. Di; Rønne, Mogens; Burguete, Isabel; Lioi, Maria Brigida; Taibi, L.; Matassino, D.

doi:10.1093/oxfordjournals.jhered.a110371

Cited by 23 publications

(18 citation statements)

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“…In the caprine chromosome complements (2n=60), the autosomes are all acrocentric with an arm ratio (long arm length/ short arm length) of 7.0-∞ [24,25]. Although the caprine X chromosome is one of the largest acrocentrices, the Y chromosome is the smallest and the only metacentric chromosome in the set [26].…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Telomere Shortening in the Cloned Caprine Cultured Cell Line, CPF-1, Derived from Placenta of Shiba Goat. (Capra hircus).

Inoue

Takanashi

Noguchi

et al. 2002

Journal of Reproduction and Development

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Abstract. As a prerequisite for the production of cloned goats by nuclear transfer from the cultured somatic cells, we established cloned fibroblast cell lines, CPF-1 and other related CPF subclones from the placenta of the Shiba goat. CPF-1 cells ceased to proliferate at and about the 51st population doubling level (PDL) when the morphological signs of senescence, such as an unusual increase in cell volume, became obvious. The mean surface area of a cell at 51PDL was about 3 times greater than that of an early-passage cell (3PDL) (p<0.001). When the CPF-1 cells at 14PDL were subjected to chromosomal analysis, 49.3% of the cells turned out to be of normal diploid chromosomal constitution (2n=60). All the chromosomes were of acrocentric types. Part of the reason for the rather high incidence of aneuploidy might be due to the well-known technical difficulty in preparing good chromosomal spreads in the caprine cells. We analyzed the rates of telomere length shortening in the CPF-1 cells, according to the number of passages and culture periods. The telomere length in the latepassage cells became approximately 1/2.5 of that of the early-passage cells (p<0.05). The estimated mean rate of telomere shortening in the CPF-1 cells was approximately 260 bases per PDL and faster than the values (50-200 bases/PDL) so far reported in the literature for other types of cells. Furthermore, in the CPF-1 cells the replicative capacity was exhausted when the mean telomere length reached approximately 7.5 kb which is significantly longer than the values generally obtained in other cultured cell lines. The reasons for the observations have not yet been clarified.

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Section: Discussionmentioning

confidence: 99%

Analysis of the Telomere Shortening in the Cloned Caprine Cultured Cell Line, CPF-1, Derived from Placenta of Shiba Goat. (Capra hircus).

Inoue

Takanashi

Noguchi

et al. 2002

Journal of Reproduction and Development

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“…Lymphocytes were synchronized by excess of thymidine and RBA-banding was induced by using a combination of BrdU and H33258, as previously reported for the R-banded karyotype of the goat (Di Berardino et al, 1987). Thymidine synchronization is achieved by a feed-back inhibition mechanism which blocks the formation of deoxycytidine triphosphate from cytidine 5-phosphate (Xeros, 1962), thus blocking the majority of the cells at mid S-phase of the cell cycle.…”

Section: Discussionmentioning

confidence: 99%

“…The potential of the RBA-banding technique for definite identification of Bovidae chromosomes, first pointed out by Popescu (1975) and by Gustavsson and Hagelthorn (1976), has subsequently been demonstrated by another series of studies on the R-banding pattern of prometaphase chromosomes of Bos taurus L. (Di Berardino et aL, 1979, 1985Di Berardino and lannuzzi, 1982), Bubalus bubalis L. lannuzzi, 1981, 1984) and Capra hircus L. (Di Berardino et al, 1987). Peripheral blood drawn from the jugular vein of 11 animals of the Gentile di Puglia breed (6 males and 5 females) was cultured for 72 h in RPMI 1640 medium (Row, Dutch modification), supplemented with 10% fetal calf serum (Gibco), antibacterial and antifungal agents, 0.1% of L-glutamine, and pokeweed mitogen (Gibco).…”

Section: Introductionmentioning

confidence: 94%

“…The karyotype has been arranged by following the same nomenclature as previously adopted for the RBA-banded karyotype of the goat (Di Berardino et al, 1987) and cattle (Di Berardino et al, 1985) which refers, as far as possible, to the Reading system (Ford et aL, 1980). Several karyotypes were prepared from the 11 investigated animals, but only the best banded chromosomes, 4 for each pair, were selected and used to produce the diagrammatic representation shown in Fig.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

R-banding pattern of the prometaphase chromosomes of the domestic sheep Ovis aries L.

Berardino¹,

Lioi²,

Miranda³

et al. 1989

Genet Sel Evol

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“…Cattle, goat and sheep karyotypes show a high degree of similarity as far as their nombre fondamental NF = 58 (number of autosome arms) and the band patterns of most of their chromosome arms are concerned (Buckland and Evans, 1978;Di Berardino et al, 1987;Mensher et al, 1989). …”

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confidence: 99%