R-Phycoerythrin from Colaconema formosanum (Rhodophyta), an Anti-Allergic and Collagen Promoting Material for Cosmeceuticals

Abstract

R-phycoerythrin (R-PE), a pigment complex found in red algae, was extracted and purified from a newly identified red alga, Colaconema formosanum, and its bioactivities were examined. It was revealed that R-PE treatment resulted in high cell viability (>70%) to the mammalian cell lines NIH-3T3, RBL-2H3, RAW264.7, and Hs68, and had no effect on cell morphology in NIH-3T3 cells. Its suppression effect was insignificant on the production of IL-6 and TNF-α in lipopolysaccharides-stimulated RAW264.7 cells. However, calcium ionophore A23187-induced β-hexosaminidase release was effectively inhibited in a dose-dependent manner in RBL-2H3 cells. Additionally, it was revealed to be non-irritating to bionic epidermal tissues. Notably, procollagen production was promoted in Hs68 cells. Overall, the data revealed that R-PE purified from C. formosanum exhibits anti-allergic and anti-aging bioactivities with no observed consequential toxicity on multiple mammalian cell lines as well as epidermal tissues, suggesting that this macromolecule is a novel material for potential cosmetic use.

Biopigments (Phycoerythrin, Fucoxanthin and Siphonaxanthin) from Seaweeds and Their Potential Applications as Ingredients in Cosmeceutical Industries: A Review

“…There are conventional and non-conventional methods for extracting biopigments (PE, FX, and SX) from seaweeds. Conventional techniques comprise maceration with buffer [46,71,82], water [20], DMSO-water [27], and organic solvents [35,39,75,[83][84][85], whereas non-conventional techniques include enzyme-assisted extraction (EAE) [21,56,86], ultrasound-assisted extraction (UAE) [49], combination of UAE and EAE [87], combination of high pressure-assisted extraction (HPAE) and UAE [72], combination of ME, UAE and HPAE [53], supercritical fluid extraction (SFE) [88], pressurized liquid extraction (PLE) [89], and microwave-assisted extraction (MAE) [90].…”

Red Seaweed Pigments from a Biotechnological Perspective

“…However, the primary commercial interest in PBP stems from the fact that these proteins offer health benefits as antioxidants and free-radical scavengers [5,[37][38][39], having a therapeutic and nutraceutical effect [40][41][42][43], as well as being effective neuroprotective, anti-bacterial [37], anti-viral [44], anti-inflammatory [45], anti-allergic [46], antitumoral [37,[47][48][49][50], anti-ageing [46], anti-Alzheimer, hepatoprotective [39], immunomodulatory [51], and hypocholesterolemia agents [4,19,42].…”

“…Common solvents applied and tested among researchers, to retrieve PE from red seaweeds, are phosphate buffer [35,46,50,58,69,70,73,78,79,89,90,93] and sodium phosphate buffer [53,103], featured prominently in Table 1. However, solvents such as potassium phosphate buffer [96], distilled water [45,77,83,91], acetic acid-sodium acetate [81,102], citrate buffer [57,74], diethyl ether [74], and EDTA [84], have also been found to be effective.…”

Comparison of Production and Fluorescence Characteristics of Phycoerythrin from Three Strains of Porphyridium

“…Currently, it is widely used as fluorescent dye. Additionally, the potential functions of phycoerythrin were reported, including modulating the gut microbiota [ 1 ], inducing apoptosis of cancer cells [ 2 ], regulating immunity [ 3 , 4 ], anti-allergy and anti-aging [ 5 ]. Thus, phycoerythrin is considered as a natural high-value product in future.…”