R-phycoerythrin from the macroalgaCorallina officinalis (Rhodophyceae) and application of a derived phycofluor probe for detecting sugar-binding sites on cell membranes

Hilditch, C. M.; Balding, P.; Jenkins, Robert E.; Smith, A. J.; Rogers, Lyndon J.

doi:10.1007/bf02392888

Cited by 37 publications

(6 citation statements)

References 18 publications

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“…ENVI's "Cross-track Illumination Correction" function produced a spectrally coherent dataset with no evident light field variation, while the subsequent conversion from upwelling spectral radiance to IARR generated "pseudo reflectance" that in terms of spectral shape closely resembled absolute reflectance. An example that supports the latter claim is the IARR signature of coralline algae in Figure 2, where distinguished dips corresponding to the in vivo absorption maxima of chlorophyll a (438 and 679 nm [30,31]) and R-phycoerythrin (497 and 566 nm [32][33][34]) clearly are present. The georeferenced IARR transect lined up well with the Figaro 3D model (Figure 11a), which implied that the time-stamped navigation data from the photogrammetry model were sufficiently accurate for geospatial positioning of push-broom hyperspectral imagery.…”

Section: Data Quality Of Underwater Hyperspectral Imagerymentioning

confidence: 55%

Mapping the Historical Shipwreck Figaro in the High Arctic Using Underwater Sensor-Carrying Robots

et al. 2020

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In 2007, a possible wreck site was discovered in Trygghamna, Isfjorden, Svalbard by the Norwegian Hydrographic Service. Using (1) a REMUS 100 autonomous underwater vehicle (AUV) equipped with a sidescan sonar (SSS) and (2) a Seabotix LBV 200 mini-remotely operated vehicle (ROV) with a high-definition (HD) camera, the wreck was in 2015 identified as the Figaro: a floating whalery that sank in 1908. The Figaro is to our knowledge currently the northernmost wreck in the world to be investigated by archaeologists. As the wreck is protected by law as an underwater cultural heritage (UCH) site, only non-intrusive methods could be used during surveys. In this study, we demonstrate how using multiple complementary remote sensing techniques can be advantageous with respect to acquiring a holistic overview of a recently discovered wreck site. In January 2016, the wreck was revisited, and a full photogrammetric survey of the site was conducted with a Sperre Subfighter 7500 medium class ROV. In addition to stereo camera images, HD-video and underwater hyperspectral imagery was also obtained from the wreck site. In terms of data analysis and interpretation, the emphasis was in the current study put on the photogrammetric 3D model and the underwater hyperspectral imagery. The former provided an excellent general overview of the Figaro wreck site, whereas the latter supplied detailed information from a 14.65-m2 sub-area situated on the top of the wreck. By analyzing classified underwater hyperspectral imagery in context with supplementary information from the 3D model, the levels of biofouling associated with different marine archaeological substrate types were assessed. Our findings suggest that strongly protruding archaeological objects support significantly higher levels of biofouling than their surroundings, and consequently that high-density biological assemblages could serve as proxies for identifying human-made artifacts on the seafloor.

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Section: Data Quality Of Underwater Hyperspectral Imagerymentioning

confidence: 55%

Mapping the Historical Shipwreck Figaro in the High Arctic Using Underwater Sensor-Carrying Robots

et al. 2020

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“…According to the ultraviolet absorption characteristics, there are mainly two types of PE in red microalga, namely, B-PE and R-phycoerythrin (R-PE). B-PE exhibits two absorption peaks at 545 and 564 nm, and one absorption shoulder at 499 nm; R-PE exhibits two characteristic absorption peaks at 499 and 564 nm, and one absorption shoulder at 545 nm (Glazer, 1984;Hilditch et al, 1991;Galland-Irmouli et al, 2000). It can be concluded that the PE fraction extracted and purified in this study is B-PE.…”

Section: Analysis Of Phycoerythrin Extractionmentioning

confidence: 63%

Integration Bioprocess of B-Phycoerythrin and Exopolysaccharides Production From Photosynthetic Microalga Porphyridium cruentum

et al. 2022

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Red microalga Porphyridium cruentum has great potential for converting CO2 into high-value bioactive compounds, such as B-phycoerythrin (B-PE) and extracellular polysaccharides or exopolysaccharides (EPS). This study aimed to establish the integration bioprocess of B-PE and EPS production from P. cruentum. First, different kinds of growth medium and CO2 concentration were assessed indoor in terms of high biomass and B-PE and EPS contents. As follows, P. cruentum cells were outdoor scale-up cultured in 700 L pressurized tubular reactors for 9 days till the biomass reached 0.85 g/L and then separated from supernatants via centrifugation. Three different methods were adopted to extract phycobiliproteins, and the highest PE contents were extracted from cells by repeated freeze-thawing treatment along with the optimization of significant variables, and finally, 7.99 mg/L B-PE (16,500 Da) with a purity index of 0.82 was obtained. Moreover, analysis of physicochemical properties of EPS extracted from P. cruentum showed that the sulfate content was 14.85% and the uronic acid content was 9.36%.

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“…PE has gained tremendous interest due to excellent spectroscopic properties. It has been extensively investigated and exploited commercially for usage such as for fluorescent dyes in bio-assay [3,4]. In this regards, OsPP might be substituted chemicals of PE.…”

Section: E3s Web Of Conferences 47mentioning

confidence: 99%

“…was sub cultured in test tubes (3 cm i.d. x 20 cm) containing 50 ml of modified C medium at pH 7.5 with the following composition: (per liter) 5 g KNO 3 4 .5H 2 O. The cells were cultivated with aeration (10 ml min -1 , 1 % CO2) at 25 o C under 50 µmol photons m -2 s -1 of continuous illumination using white fluorescence lamps.…”

Section: Algal Cultivation Conditionmentioning

confidence: 99%

Effect of medium and light quality on pink pigment production of cyanobacteria Oscillatoria sp. BTCC/A0004

2018

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Abstract. Cyanobacteria are well known as promising source of valuable chemicals for human usage. Especially, cyanobacteria in tropical area are very wide in diversity and they are potent producers of unique metabolites which exhibit interesting bioactivities. Oscilatoria sp. BTCC/A0004 produce pink pigments extracellularly (OsPP). The effects of various environmental factors on the production of cyanobacteria metabolites were well documented. In this research, the effect of medium and light quality on cell growth and OsPP production were investigated. In case, three different culture media, named No 18, C, and modified C media, in which nutrient compositions are different, and light quality (white, blue, green, pink) were tested. The highest cell growth and OsPP production were obtained in modified C medium.

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R-phycoerythrin from the macroalgaCorallina officinalis (Rhodophyceae) and application of a derived phycofluor probe for detecting sugar-binding sites on cell membranes

Cited by 37 publications

References 18 publications

Mapping the Historical Shipwreck Figaro in the High Arctic Using Underwater Sensor-Carrying Robots

Mapping the Historical Shipwreck Figaro in the High Arctic Using Underwater Sensor-Carrying Robots

Integration Bioprocess of B-Phycoerythrin and Exopolysaccharides Production From Photosynthetic Microalga Porphyridium cruentum

Effect of medium and light quality on pink pigment production of cyanobacteria Oscillatoria sp. BTCC/A0004

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