R-plasmid transfer in soil and water

Trevors, J. T.; Oddie, K.M.

doi:10.1139/m86-114

Cited by 87 publications

(35 citation statements)

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“…According to the results of the present study, incubation temperatures of 4 and 15°C did not favor the transfer of integron-mediated antibiotic resistance genes in bovine feces and storm water. Previous studies showed that a temperature higher than 15°C was required for conjugal transfer of R-plasmids between E. coli cells (21,23). Singleton and Anson (21) found that transfer of an R-plasmid between E. coli cells occurred at 17 to 37°C when they studied the transfer of the plasmid at six different temperatures, 15, 17, 20, 22, 27, and 37°C; no transconjugants were obtained when the conjugation experiment was conducted at 15°C.…”

Section: Discussionmentioning

confidence: 99%

Transfer of Class 1 Integron-Mediated Antibiotic Resistance Genes from Shiga Toxin-Producing Escherichia coli to a Susceptible E. coli K-12 Strain in Storm Water and Bovine Feces

Nagachinta

Chen

2008

Appl Environ Microbiol

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Transfer of class 1 integron-mediated antibiotic resistance genes has been demonstrated under laboratory conditions. However, there is no information concerning the transfer of these genes in an agricultural environment. The present study sought to determine if integron-mediated streptomycin and sulfisoxazole resistance genes could be transferred from Shiga toxin-producing Escherichia coli (STEC) strains 6-20 (O157: H7) and 7-63 (O111:H8) to the susceptible strain E. coli K-12 MG1655 in bovine feces (pH 5.5, 6.0, or 6.5) and storm water (pH 5, 6, 7, or 8) at 4, 15, and 28°C, which are average seasonal temperatures for winter, spring-fall, and summer, respectively, in the Griffin, GA, area. The results indicated that at 28°C, the integron-mediated antibiotic resistance genes were transferred from both of the STEC donors in bovine feces. Higher conjugation efficiencies were, however, observed in the conjugation experiments involving STEC strain 6-20. In storm water, the resistance genes were transferred only from STEC strain 6-20. Greater numbers of transconjugants were recovered in the conjugation experiments performed with pH 6.5 bovine feces and with pH 7 storm water. Antibiotic susceptibility tests confirmed the transfer of integron-mediated streptomycin resistance and sulfisoxazole resistance, as well as the transfer of non-integron-mediated oxytetracycline resistance and tetracycline resistance in the transconjugant cells. These results suggest that the antibiotic resistance genes in STEC could serve as a source of antibiotic resistance genes disseminated via conjugation to susceptible cells of other E. coli strains in an agricultural environment.

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Section: Discussionmentioning

confidence: 99%

Transfer of Class 1 Integron-Mediated Antibiotic Resistance Genes from Shiga Toxin-Producing Escherichia coli to a Susceptible E. coli K-12 Strain in Storm Water and Bovine Feces

Nagachinta

Chen

2008

Appl Environ Microbiol

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“…The effects of environmental parameters (including temperature, pH, nutrient concentration, salinity and metal ions) on plasmid transfer have been previously investigated by a number of other groups (Singleton & Anson, 1981 ;Gauthier e t al., 1985;Trevors & Oddie, 1986;Khalil & Gealt, 1987;Van Elsas e t al., 1987;Rochelle e t al., 1989;Fernandez-Astorga e t al., 1992). The approach of all these studies differed from that of the present paper in that they measured the frequency of plasmid transfer in response to variation of environmental factors.…”

Section: Discussionmentioning

confidence: 99%

The use of two-dimensional gradient plates to investigate the range of conditions under which conjugal plasmid transfer occurs

Venables

Wimpenny²,

Ayres³

et al. 1995

Microbiology

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Gel-stabilized two-dimensional gradient plates were used to study the effects of pH, salt concentration and temperature on the conjugal transfer of plasmid RP4 between strains of Escherichia coli and Pseudomonas putida. The combinations of pH and salt concentration that permitted conjugation were mapped as a two-dimensional growth area occupied by transconjugants following conjugation. This conjugation domain was less extensive than the areas that supported growth of the parental strains, and showed evidence for the interactive effects of pH and salt concentration in determination of conditions that permitted conjugation. The size and shape of the conjugation domain was influenced by time, temperature, the identities of the donor and recipient bacteria, and the combination of donor and recipient bacteria.

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“…Biotic factors such as competition, antagonism and prédation might also affect transfer frequencies. It should be stressed that, so far, most data on plasmid transfer in soil have been obtained using a system of introduced donor and recipient bacteria and mobilizable or broad-host-range self-transmissible plasmids [17,[34][35][36][37]. These data represent a "worst case scenario", rather unrealistic for the planned releases in which heterologous DNA will mainly be present on the chromosome.…”

Section: Genetic Stability and Gene Tvansfermentioning

confidence: 99%

Environmental risks and fate of genetically engineered microorganisms in soil

Elsas¹,

Trevors

1991

Journal of Environmental Science and Health . Part A: Environme

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Genetically engineered microorganisms in the environment will prove to be useful if they provide a means by which to perform a desirable function in the environment, whilst not posing unacceptable risks to any component of the environment. Moreover, the uncertainty associated with the environmental release of living organisms due to the high density of cells that will be released and the capability of the organisms to grow, reproduce and undergo genetic modifications creates a unique challenge. To minimize risks, a thorough understanding of the ecology, physiology and genetics of the organism under consideration for release, in realistic environmental conditions must be available. In addition, the environment into which the organism is to be released must also be wellcharacterized with respect to its influence on the introduced organism and the effect of the released organism on the target environment.This review examines potential risks to the environment should genetically engineered organisms be released and methods useful in assessing their fate once they are released. It includes organism survival,

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R-plasmid transfer in soil and water

Cited by 87 publications

References 0 publications

Transfer of Class 1 Integron-Mediated Antibiotic Resistance Genes from Shiga Toxin-Producing Escherichia coli to a Susceptible E. coli K-12 Strain in Storm Water and Bovine Feces

Transfer of Class 1 Integron-Mediated Antibiotic Resistance Genes from Shiga Toxin-Producing Escherichia coli to a Susceptible E. coli K-12 Strain in Storm Water and Bovine Feces

The use of two-dimensional gradient plates to investigate the range of conditions under which conjugal plasmid transfer occurs

Environmental risks and fate of genetically engineered microorganisms in soil

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