R-spondin1 and FOXL2act into two distinct cellular types during goat ovarian differentiation

Kocer, Ayhan; Pinheiro, Iris; Pannetier, Maëlle; Renault, Ludovic; Parma, P.; Radi, Orietta; Kim, Kyung-Ah; Camerino, Giovanna; Pailhoux, Eric

doi:10.1186/1471-213x-8-36

Cited by 67 publications

(67 citation statements)

References 49 publications

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“…Thus, RSPO1 appears to activate canonical b-catenin signaling in both somatic cells and germ cells of the ovary . Consistent with this model, RSPO1 protein is detected on the membrane of both cell types (Kocer et al 2008, Smith et al 2008, suggesting that CTNNB1 is directly activated in female germ cells to regulate their fate. Consistent with this hypothesis, germ cells of the XX embryos normally enter meiosis when Ctnnb1 is specifically ablated in somatic cells of the developing ovary (Sf1:cre; Ctnnb1 flox/flox mice; Manuylov et al 2008, Liu et al 2009).…”

Section: Rspo1 and Wnt4 Regulate Meiosis Entry Of Female Germ Cells supporting

confidence: 67%

“…RSPO1 and WNT4 are secreted by the somatic cells of the ovary. In addition, RSPO1 expression is detected at the membrane of both somatic and germ cells at 12.5 and 14.5 dpc (Kocer et al 2008, Smith et al 2008, suggesting that it may act on both cell types (see below).…”

Section: R99mentioning

confidence: 99%

“…In goats, RSPO1 is expressed in both male and female mesonephroi and the level of expression peaks just before germ cell entry into meiosis (55 dpc; Kocer et al 2008). In addition to RSPO1, RSPO2 is expressed at the time of folliculogenesis (from 70 dpc until before birth) in goat gonads, while RSPO4 begins to be very faintly expressed in 50 dpc ovaries (Kocer et al 2008).…”

Section: Mammalsmentioning

confidence: 99%

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R-spondin1, WNT4, and the CTNNB1 signaling pathway: strict control over ovarian differentiation

Chassot¹,

Gillot²,

Chaboissier³

2014

Reproduction

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Sex differentiation is a unique developmental process. Starting from a bipotential gonad, it gives rise to the ovary and the testis, two highly specialized organs that differ morphologically and physiologically despite sharing common reproductive and endocrine functions. This highlights the specific plasticity of the gonadal precursors and the existence of complex antagonistic genetic regulation. Mammalian sex determination is controlled by paternal transmission of the Y-linked gene, sex-determining region Y (SRY). Using mouse models, it has been shown that the main role of Sry is to activate the expression of the transcription factor Sox9; either one of these two genes is necessary and sufficient to allow testicular development through Sertoli cell differentiation. Thus, defects in SRY/Sry and/or SOX9/Sox9 expression result in male-to-female sex reversal of XY individuals. Molecular mechanisms governing ovarian differentiation remained unknown for a long time, until the discovery of the roles of R-spondin1 (RSPO1) and WNT4. In XX individuals, activation of the b-catenin signaling pathway by the secreted proteins RSPO1 and WNT4 is required to allow granulosa cell differentiation and, in turn, ovarian differentiation. Thus, mutations in RSPO1 result in female-to-male sex reversal of XX patients, and mouse models have allowed the identification of genetic cascades activated by RSPO1 and WNT4 to regulate ovarian development. In this review, we will discuss the respective roles of RSPO1, WNT4, and the b-catenin signaling pathway during ovarian differentiation in mice.Reproduction (2014) 148 R97-R110

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Section: Rspo1 and Wnt4 Regulate Meiosis Entry Of Female Germ Cells supporting

confidence: 67%

Section: R99mentioning

confidence: 99%

Section: Mammalsmentioning

confidence: 99%

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R-spondin1, WNT4, and the CTNNB1 signaling pathway: strict control over ovarian differentiation

Chassot¹,

Gillot²,

Chaboissier³

2014

Reproduction

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“…Forty-eight hours after transfection, the cells were fixed in 4% paraformaldehyde in PBS for 10 min and immunocytochemistry was performed as previously described (Kocer et al, 2008). For the antibodies used, see Supp.…”

Section: Immunofluorescence On Cos-7 Cellsmentioning

confidence: 99%

A study of goat SRY protein expression suggests putative new roles for this gene in the developing testis of a species with long‐lasting SRY expression

Montazer-Torbati

Kocer

Auguste

et al. 2010

Developmental Dynamics

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The testis-determining gene SRY is not well-conserved among mammals, and particularly between mouse and other mammals. To evaluate SRY function in a nonrodent species, we produced an antibody against goat SRY and used it to investigate the expression pattern of SRY throughout goat testicular development. By contrast with the mouse, SRY is primarily expressed in most cells of XY genital-ridges and not solely in pre-Sertoli cells. Between cord formation and prepuberty, SRY remains expressed in both Sertoli and germinal cells. During adulthood, SRY expression declines and then disappears from meiotic germ cells, only remaining present at low levels in some spermatogonia. Unlike the germinal lineage, SRY continues to be highly expressed in adult Sertoli cells with a typical nuclear staining. Our data indicate that in goat, the role of SRY may not be limited to testis determination and could have other functions in testicular maintenance and hence male fertility. Developmental Dynamics 239:3324-3335,

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“…38 R-spondin1 and FOXL2 act in two distinct cellular types during goat ovarian differentiation. 39 This interaction appears critical for early genital development and ovarian determination. Mutations in the R-spondin1 (RSPO1) gene have been associated with 46,XX testicular DSD in the absence of the testis-determining gene SRY, as well as with ovotesticular DSD in a 46,XX individual.…”

mentioning

confidence: 99%

Sex determination and disorders of sex development according to the revised nomenclature and classification in 46,XX individuals

Kousta¹,

Papathanasiou²,

Skordis³

2010

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There have been considerable advances concerning understanding of the early and later stages of ovarian development; a number of genes have been implicated and their mutations have been associated with developmental abnormalities. The most important genes controlling the initial phase of gonadal development, identical in females and males, are Wilms' tumor suppressor 1 (WT1) and steroidogenic factor 1 (SF1). Four genes are likely to be involved in the subsequent stages of ovarian development (WNT4, DAX1, FOXL2 and RSPO1), but none is yet proven to be the ovarian determining factor. changes in nomenclature and classification were recently proposed in order to incorporate genetic advances and substitute gender-based diagnostic labels in terminology. The term "disorders of sex development" (DsD) is proposed to substitute the previous term "intersex disorders". Three main categories have been used to describe DsD in the 46,XX individual: 1) disorders of gonadal (ovarian) development: ovotesticular DsD, previously named true hermaphroditism, testicular DsD, previously named XX males, and gonadal dysgenesis; 2) disorders related to androgen excess (congenital adrenal hyperplasia, aromatase deficiency and P450 oxidoreductase deficiency); and 3) other rare disorders. In this mini-review, recent advances concerning development of the genital system in 46,XX individuals and related abnormalities are discussed. basic embryology of the ovary and molecular pathways determining ovarian development are reviewed, focusing on mutations disrupting normal ovarian development. Disorders of sex development according to the revised nomenclature and classification in 46,XX individuals are summarized, including genetic progress in the field.

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R-spondin1 and FOXL2act into two distinct cellular types during goat ovarian differentiation

Cited by 67 publications

References 49 publications

R-spondin1, WNT4, and the CTNNB1 signaling pathway: strict control over ovarian differentiation

R-spondin1, WNT4, and the CTNNB1 signaling pathway: strict control over ovarian differentiation

A study of goat SRY protein expression suggests putative new roles for this gene in the developing testis of a species with long‐lasting SRY expression

Sex determination and disorders of sex development according to the revised nomenclature and classification in 46,XX individuals

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