R-spondins are involved in the ovarian differentiation in a teleost, medaka (Oryzias latipes)

Zhou, Lei; Charkraborty, Tapas; Yu, Xiangguo; Wu, Limin; Liu, Gang; Mohapatra, Sipra; Wang, Deshou; Nagahama, Yoshitaka

doi:10.1186/1471-213x-12-36

Cited by 51 publications

(36 citation statements)

References 43 publications

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“…latipes , and D . rerio (Parma et al., ; Smith et al., ; Tomaselli et al., ; Zhang et al., ; Zhou, Charkraborty, & Yu, ), indicating a conserved expression pattern and potentially conserved function in vertebrates. The preceding observations and the findings of the present study suggest that rspo1 might participate in both development and maintenance of female traits, since it was expressed at almost all developmental stages of the female gonads (Figure ).…”

Section: Discussionmentioning

confidence: 99%

Characterization and expression pattern of r‐spondin1 in Cynoglossus semilaevis

Zhu

et al. 2017

J Exp Zool Pt B

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r-spondin1 (rspo1) encodes a secreted protein that is involved in the determination and differentiation of the mammalian ovary. However, little information is yet available for teleosts. Here, we identified a homologue of rspo1 in Cynoglossus semilaevis. The full-length cDNA of rspo1 had a length of 2,703 bp with an open reading frame of 834 bp, encoding a protein with a length of 277 amino-acids. rspo1 expression was detected via qRT-PCR in various tissues, and significant sexually dimorphic expression was observed in the gonads. Furthermore, ISH located rspo1 in germ cells such as spermatogonia, spermatocytes, spermatids, spermatozoa, and oocytes, as well as in somatic cells of the gonads. Following knockdown of rspo1 in an ovarian cell line, the expressions of wnt4a, β-catenin, foxl2, and StAR were highly affected; wnt4a and β-catenin were significantly downregulated, whereas foxl2 and StAR were significantly upregulated. In summary, these data suggest that rspo1 may be involved in the regulation of ovarian development and differentiation through a conserved pathway, while the function of the gene in the testis remains elusive.

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Section: Discussionmentioning

confidence: 99%

Characterization and expression pattern of r‐spondin1 in Cynoglossus semilaevis

Zhu

et al. 2017

J Exp Zool Pt B

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“…Moreover, transgenic inhibition of wnt signaling results in male-biased sex ratios, suggesting that Wnt signaling is a conserved key pathway during gonadal differentiation in zebrafish (Sreenivasan et al 2014). In medaka (Oryzias latipes), Rspo1 and Rspo2 are prominently expressed in both germ cells and somatic cells (Zhou et al 2012). Their functions in this species remain yet to be clarified.…”

Section: Teleost Fishesmentioning

confidence: 99%

R-spondin1, WNT4, and the CTNNB1 signaling pathway: strict control over ovarian differentiation

Chassot¹,

Gillot²,

Chaboissier³

2014

Reproduction

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Sex differentiation is a unique developmental process. Starting from a bipotential gonad, it gives rise to the ovary and the testis, two highly specialized organs that differ morphologically and physiologically despite sharing common reproductive and endocrine functions. This highlights the specific plasticity of the gonadal precursors and the existence of complex antagonistic genetic regulation. Mammalian sex determination is controlled by paternal transmission of the Y-linked gene, sex-determining region Y (SRY). Using mouse models, it has been shown that the main role of Sry is to activate the expression of the transcription factor Sox9; either one of these two genes is necessary and sufficient to allow testicular development through Sertoli cell differentiation. Thus, defects in SRY/Sry and/or SOX9/Sox9 expression result in male-to-female sex reversal of XY individuals. Molecular mechanisms governing ovarian differentiation remained unknown for a long time, until the discovery of the roles of R-spondin1 (RSPO1) and WNT4. In XX individuals, activation of the b-catenin signaling pathway by the secreted proteins RSPO1 and WNT4 is required to allow granulosa cell differentiation and, in turn, ovarian differentiation. Thus, mutations in RSPO1 result in female-to-male sex reversal of XX patients, and mouse models have allowed the identification of genetic cascades activated by RSPO1 and WNT4 to regulate ovarian development. In this review, we will discuss the respective roles of RSPO1, WNT4, and the b-catenin signaling pathway during ovarian differentiation in mice.Reproduction (2014) 148 R97-R110

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“…The amh ( anti‐mullerian hormone ) and its receptor ( anti‐mullerian hormone receptor : amhr ), igf1 ( insulin‐like growth factor 1 ) and its receptor ( insulin‐like growth factor 1 receptor : igf1r ), and rspo1 ( r‐spondin 1 ) are thought to be involved in gonadal differentiation (e.g. Rodríguez‐Marí et al., ; Schlueter et al., ; Zhou et al., ). This also appears to be the case for gonadotropins ( follicle stimulating hormone : fsh and luteinizing hormone : lh ) and their receptors ( follicle stimulating hormone receptor : fshr and luteinizing hormone receptor : lhr ) (e.g.…”

Section: Introductionmentioning

confidence: 99%

Identification of genes involved in gonadal sex differentiation and the dimorphic expression pattern in undifferentiated gonads of Russian sturgeonAcipenser gueldenstaedtiiBrandt & Ratzeburg, 1833

et al. 2014

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The establishment of a sexing technique at an early developmental stage is an important issue in sturgeon aquaculture, yet the mechanism of sex differentiation in sturgeons remains poorly understood. This study aimed to identify genes involved in gonadal sex differentiation and to investigate sexually dimorphic gene expression in undifferentiated gonads. RNA-seq analyses using next-generation sequencers were carried out on undifferentiated gonads of five 9-month-old juvenile Russian sturgeon Acipenser gueldenstaedtii. A total of 45 686 832 (8498 Mb) and 79 743 269 (7887 Mb) qualitycontrolled reads were obtained using Ion PGM and HiSeq 2000 sequencers, respectively, and were assembled into 338 648 contigs (N50: 532 b). tBLASTx analyses identified 26 transcripts potentially involved in gonadal differentiation. Read-mapping analyses were performed to obtain relative transcript levels (RPKM values). Multivariate analyses of the RPKM values of five transcripts (gsdf, dmrt1a, dmrt1b, foxl2 and cyp19a1a) showed that the five individuals could be separated into two distinct groups. One group, comprising two individuals, had increased levels of foxl2, hsd17b1 and cyp19a1a, suggesting differentiation into females. Alternatively, levels of gsdf were higher in the other three individuals, suggesting differentiation into males. We describe the levels of 26 transcripts potentially involved in gonadal differentiation in undifferentiated sturgeon gonads and suggest that levels of gsdf, foxl2, hsd17b1 and cyp19a1a are appropriate sexing markers for sturgeon at an early developmental stage. U.S.

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R-spondins are involved in the ovarian differentiation in a teleost, medaka (Oryzias latipes)

Cited by 51 publications

References 43 publications

Characterization and expression pattern of r‐spondin1 in Cynoglossus semilaevis

Characterization and expression pattern of r‐spondin1 in Cynoglossus semilaevis

R-spondin1, WNT4, and the CTNNB1 signaling pathway: strict control over ovarian differentiation

Identification of genes involved in gonadal sex differentiation and the dimorphic expression pattern in undifferentiated gonads of Russian sturgeonAcipenser gueldenstaedtiiBrandt & Ratzeburg, 1833

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