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Ichiki, Katsuyuki; Mitani, Noriyasu; Doki, Yoshinori; Hara, Hiroiku; Misaki, Takuro; Saiki, Ikuo

doi:10.1023/a:1011980313237

Cited by 38 publications

(15 citation statements)

References 33 publications

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“…1). This is highly consistent with our previous observation in mediastinal lymph node metastases of orthotopically implanted Lewis lung carcinoma cells, in which curcumin inhibited the metastasis but not the primary tumor growth [16]. These results suggest that curcumin targeted metastasis-specific processes.…”

Section: Discussionsupporting

confidence: 93%

“…Curcumin, a natural product of the Indian spice turmeric isolated from Curcuma longa , has potent anti-inflammatory [12], anti-carcinogenic [13]and anti-metastatic [14, 15, 16]activities. It has been shown that the suppression of AP-1 transcriptional activity is one major part of the activities of curcumin [17].…”

Section: Introductionmentioning

confidence: 99%

“…The AP-1 DNA-binding sites were identified in the promoter regions of mouse MMP-9 [18]and integrins [19], and play pivotal roles in their gene expression. We have previously reported that curcumin inhibited mediastinal lymph node metastasis of orthotopically implanted Lewis lung carcinoma [16]. However, the detailed anti-metastatic mechanism of curcumin remains unclear.…”

Section: Introductionmentioning

confidence: 99%

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Prevention of Intrahepatic Metastasis by Curcumin in an Orthotopic Implantation Model

et al. 2003

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Curcumin has been shown to have potent anti-metastatic activity, however, its mechanism of action is still unclear. Here, we analyzed the anti-metastatic mechanism using hepatocellular carcinoma, CBO140C12 cells. Daily oral administration of curcumin suppressed intrahepatic metastasis in a dose-dependent manner, whereas the growth of implanted tumors was not affected. We next examined the effect of curcumin on several metastatic properties in vitro. Curcumin inhibited the invasion of tumor cells through Matrigel-coated filters and the production of MMP-9. In addition, curcumin significantly inhibited adhesion and haptotactic migration to fibronectin and laminin without affecting the expression of integrins on the cell surface. Furthermore, the formation of actin stress fibers was affected by treatment with curcumin. These results suggested that curcumin suppressed the intrahepatic metastasis mediated by the inhibiton of several metastatic properties, in which the functional alteration of cytoskeletal organization, at least in part, could play an important role.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevention of Intrahepatic Metastasis by Curcumin in an Orthotopic Implantation Model

et al. 2003

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“…It was found that 24 to 30-hr treatments reduced the number of cells in a dose-dependent manner, which is in agreement with published results using other cell lines 1 as well as LLC 37. However, a shorter treatment interval (2 or 4 hrs) significantly reduced the density of cells only when they were treated with the highest curcumin dose (60 µM).…”

Section: Discussionsupporting

confidence: 90%

“…We selected a well studied lung cancer cell line to evaluate the use of curcumin at concentrations that are known to be cytotoxic to many different cancer cells 36. Previously, when LLC cells were used in an orthotopic tumor model, with the cells implanted in the lungs, curcumin administered orally had no effect on tumor growth but did minimize metastasis to lymph nodes near the lungs 37, suggesting that curcumin or its metabolites had some effects despite low bioavailability following ingestion.…”

Section: Discussionmentioning

confidence: 99%

Properties of Lewis Lung Carcinoma Cells Surviving Curcumin Toxicity

Yan¹,

Geusz²,

Jamasbi³

2012

J. Cancer

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The anti-inflammatory agent curcumin can selectively eliminate malignant rather than normal cells. The present study examined the effects of curcumin on the Lewis lung carcinoma (LLC) cell line and characterized a subpopulation surviving curcumin treatments. Cell density was measured after curcumin was applied at concentrations between 10 and 60 μM for 30 hours. Because of the high cell loss at 60 μM, this dose was chosen to select for surviving cells that were then used to establish a new cell line. The resulting line had approximately 20% slower growth than the original LLC cell line and based on ELISA contained less of two markers, NF-κB and ALDH1A, used to identify more aggressive cancer cells. We also injected cells from the original and surviving lines subcutaneously into syngeneic C57BL/6 mice and monitored tumor development over three weeks and found that the curcumin surviving-line remained tumorigenic. Because curcumin has been reported to kill cancer cells more effectively when administered with light, we examined this as a possible way of enhancing the efficacy of curcumin against LLC cells. When LLC cells were exposed to curcumin and light from a fluorescent lamp source, cell loss caused by 20 μM curcumin was enhanced by about 50%, supporting a therapeutic use of curcumin in combination with white light. This study is the first to characterize a curcumin-surviving subpopulation among lung cancer cells. It shows that curcumin at a high concentration either selects for an intrinsically less aggressive cell subpopulation or generates these cells. The findings further support a role for curcumin as an adjunct to traditional chemical or radiation therapy of lung and other cancers.

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