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Matyushichev, V. B.; Шамратова, В. Г.; Muzafarova, D. A.

doi:10.1023/a:1007123811532

Cited by 6 publications

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“…For instance, when studying the correlation between EPM and volume, we did not consider the intensity of metabolism and, in particular, activity of ion transport systems involved in the autoregulation of erythrocyte volume [7] and EPM control [5]. It can be assumed that young and functionally mature cells have high EPM because of high metabolic activity and energy supply rather than large size.…”

Section: Resultsmentioning

confidence: 99%

Dynamics of correlation between erythrocyte electrophoretic mobility and volume in stress

1999

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At rest, rat erythrocyte electrophoretic mobility varied independently on cell volume. Emotional and physical stress gave rise to a short-lived moderate negative correlation between erythrocyte volume and the coefficient of asymmetry of electrophoretic mobility distribution, probably originating from accompanying metabolic and systemic influences.Key Words: erythrocyte electrophoretic mobility; erythrocyte volume; rats; stress; correlation Electrophoretic mobility (EPM) of erythrocytes as a function of their surface charge is a vitally important parameter determining cell aggregation resistance, blood fluidity, and microcirculation in capillaries. It is of interest, therefore, to identify the factors participating in EPM regulation. Cell volume may be one of such factors [7]. Surface charge is proportional to cell size [11]. Young erythrocytes have higher mobility compared to old cells [ 10]. Young mature erythrocytes are larger than aging cells [2], and therefore are characterized by higher EPM, To clarify this question we studied the correlation between EPM and erythrocyte volume using the model of emotional and physical stress in rats. MATERIALS AND METHODSExperiments were carried out on 30 outbred male albino rats weighing 160-180 g. The animals were forced to swim in ice-cold water until exhaustion and decapitated under ether narcosis 10 min (n=15) and 1 h (n=15) after swimming session. EPM parameters, erythrocyte and reticulocyte count, hematocrit, and mean erythrocyte volume were determined. The conInstitute of Physiology, St. Petersburg State University; Bashkirian State University, Ufa; Institute of Evolutional Physiology and Biochemistry, Russian Academy of Sciences, St, Petersburg trol group (n=10) comprised intact rats of the same weight.Erythrocyte EPM was measured automatically with a "Parmoquant-2" apparatus at 25~ in a 0.02-ml blood sample diluted (1:200) with 0.1 M phosphate buffer (pH=7.4). Electrophoretic scanograms were input to a computer. The mean EPM for each sample was calculated by averaging data of 250 individual measurements. Coefficients of asymmetry (As) and excess (Ex) for EPM distribution were determined for each sample and for the whole groups. The significance of differences was evaluated by Student's t test. Correlation analysis was performed using standard software.

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Section: Resultsmentioning

confidence: 99%

Dynamics of correlation between erythrocyte electrophoretic mobility and volume in stress

1999

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“…Blood samples were taken from intact albino male rats (n=72, 160-200 g) and animals exposed to cold stress (n=293) [4]. Specific features of treatment are not described in the manuscript.…”

Section: Methodsmentioning

confidence: 99%

Effect of blood reticulocyte concentration on electrophoretic mobility of erythrocytes in rats

Matyushichev

Шамратова

2004

Bull Exp Biol Med

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In intact rats electrokinetic characteristics of erythrocytes weakly depended on the intensity of erythropoiesis. This dependence became significant when the degree of erythropoiesis approached the upper limit of variations. Electrophoretic mobility of erythrocytes progressively decreased with increasing blood reticulocyte count. Subpopulations of erythrocytes with low mobility in electric field accumulated in the blood. Our results indicate that erythrocytes whose electrokinetic potential is similar to the mean mobility of circulating cells migrate from the bone marrow into the circulation under normal conditions.

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“…Men and women differ in their erythrocyte concentration, hemoglobin (Hb) content, cell size [4], some biochemical indices [2], and electrophoretic mobility [5]. Given the fact that erythrocyte population is heterogenous and consists of cells with different properties, it remains unclear whether the difference in average indices can be explained by qualitative specificity of male and female erythrocytes or just by a different subpopulation structure.…”

Section: Abstract: Erythrocytes; Functional Characteristics; Volume;mentioning

confidence: 99%

Qualitative difference between male and female erythrocytes

et al. 1999

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Erythrocytes in men and women were characterized by similar distribution of cell volumes and their mean values, but considerably differed in the correlations of volumetric parameters with cell count, hematocrit, and hemoglobin content. The factorial structure of the correlations showed sex-related specificity. These data suggest that male and female erythrocytes are qualitatively different. Key Words: erythrocytes; functional characteristics; volume; correlation; sex-related specificityCharacteristics of human blood erythrocytes are known to be sex-specific. Men and women differ in their erythrocyte concentration, hemoglobin (Hb) content, cell size [4], some biochemical indices [2], and electrophoretic mobility [5]. Given the fact that erythrocyte population is heterogenous and consists of cells with different properties, it remains unclear whether the difference in average indices can be explained by qualitative specificity of male and female erythrocytes or just by a different subpopulation structure. The problem of the sex-related specificity of erythrocytes has never been considered in this way, and this work was designed to analyze it by studying the correlations between the main functional characteristics of these cells in men and women. MATERIALS AND METHODSBlood samples from healthy men (n=14) and women (n=24) aged 35-50 years were analyzed with a Sysmex NE-1500 analyzer. The following parameters were determined: erythrocyte count, Hb, hematocrit, mean Hb content per erythrocyte, mean erythrocyte volume.Institute of Physiology of St. Petersburg State University; Bashkiriya State University, Ufa; Institute of Evolutional Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg Standard deviation, coefficients of variation, asymmetry (As), and excess (Ex) were calculated from the volume distribution histograms. Student's t test was used to evaluate the difference between the mean values. Correlation analysis was performed using "Statistica" software.

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Cited by 6 publications

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Dynamics of correlation between erythrocyte electrophoretic mobility and volume in stress

Dynamics of correlation between erythrocyte electrophoretic mobility and volume in stress

Effect of blood reticulocyte concentration on electrophoretic mobility of erythrocytes in rats

Qualitative difference between male and female erythrocytes

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