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Кузнецова, Н. П.; Gudkin, L. R.; Mishaeva, R. N.

doi:10.1023/a:1020353200456

Cited by 1 publication

(2 citation statements)

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“…It is established that protein stabilization by using chemical crosslinking in neutral aqueous solutions forms stable Schiff bases. These Schiff bases formed do not dissociate because of the parallel aldol condensation of aldehydes or other chemicals to give α, β‐unsaturated oligomers immobilized on a protein molecule . Such crosslinking treatments have shown to decrease its biodegradability nature.…”

Section: Introductionmentioning

confidence: 99%

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Stabilization of collagen through bioconversion: An insight in protein–protein interaction

et al. 2014

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Collagen is a natural protein, which is used as a vital biomaterial in tissue engineering. The major concern about native collagen is lack of its thermal stability and weak resistance to proteolytic degradation. In this scenario, the crosslinking compounds used for stabilization of collagen are mostly of chemical nature and exhibit toxicity. The enzyme mediated crosslinking of collagen provides a novel alternative, nontoxic method for stabilization. In this study, aldehyde forming enzyme (AFE) is used in the bioconversion of hydroxylmethyl groups of collagen to formyl groups that results in the formation of peptidyl aldehyde. The resulted peptidyl aldehyde interacts with bipolar ions of basic amino acid residues of collagen. Further interaction leads to the formation of conjugated double bonds (aldol condensation involving the aldehyde group of peptidyl aldehyde) within the collagen. The enzyme modified collagen matrices have shown an increase in the denaturation temperature, when compared with native collagen. Enzyme modified collagen membranes exhibit resistance toward collagenolytic activity. Moreover, they exhibited a nontoxic nature. The catalytic activity of AFE on collagen as a substrate establishes an efficient modification, which enhances the structural stability of collagen. This finds new avenues in the context of protein-protein stabilization and discovers paramount application in tissue engineering.

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Section: Introductionmentioning

confidence: 99%

“…Such crosslinking treatments have shown to decrease its biodegradability nature. Moreover, they impart some degree of toxicity before cell seeding . Enzymes play a vital role in self‐assembling of collagen during biosynthesis.…”

Section: Introductionmentioning

confidence: 99%