Rab10 is Involved in Basolateral Transport in Polarized Madin–Darby Canine Kidney Cells

Schuck, Sebastian; Gerl, Mathias J.; Ang, Agnes; Manninen, Aki; Keller, Patrick; Mellman, Ira; Simons, Kai

doi:10.1111/j.1600-0854.2006.00506.x

Cited by 114 publications

(129 citation statements)

References 46 publications

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“…The fusion event may further be regulated by the mammalian homolog of drosophila tumor suppressor lethal giant larvae (lgl) [46]. Furthermore, the small GTPases Cdc42, Rab10 and Rab8 have been implicated in VSVG sorting along the biosynthetic pathway, most likely in sorting from recycling endosomes or the TGN [47][48][49]. Interestingly, Cdc42 also has a well-established function at the tight junctions where activated Cdc42 binds to the Par3/Par6/aPKC complex [50].…”

Section: Sorting Through Recycling Endosomesmentioning

confidence: 99%

Regulation of membrane trafficking in polarized epithelial cells

Fölsch¹

2008

Current Opinion in Cell Biology

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Polarized epithelial cells continuously sort transmembrane proteins to either apical or basolateral plasma membrane domains. Research in recent years has made tremendous progress in understanding the molecular mechanisms of the major pathways to either basolateral or apical domain. This understanding will help us elucidating how these pathways are interconnected in ensuring maintenance of cell polarity and integrity of epithelial monolayers.

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Section: Sorting Through Recycling Endosomesmentioning

confidence: 99%

Regulation of membrane trafficking in polarized epithelial cells

Fölsch¹

2008

Current Opinion in Cell Biology

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“…1A). Interestingly, members of this functional group, which did not localize to the SGs (i.e., Rab8A, Rab10, Rab11A, Rab12, Rab20, and Rab22A), are all implicated in regulating transport from the endocytic recycling compartment (ERC) (26,29,(36)(37)(38)(39), a discrete pericentriolar endosomal organelle, implicated in slow endocytic recycling (29).…”

Section: Functional Impacts Of Rab Gtpases and Their Phenotypic Corrementioning

confidence: 99%

Decoding the Regulation of Mast Cell Exocytosis by Networks of Rab GTPases

Azouz

Matsui

Fukuda

et al. 2012

The Journal of Immunology

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Exocytosis is a key event in mast cell functions. By this process, mast cells release inflammatory mediators, contained in secretory granules (SGs), which play important roles in immunity and wound healing but also provoke allergic and inflammatory responses. The mechanisms underlying mast cell exocytosis remained poorly understood. An essential step toward deciphering the mechanisms behind exocytosis is the identification of the cellular components that regulate this process. Because Rab GTPases regulate specific trafficking pathways, we screened 44 Rabs for their functional impacts on exocytosis triggered by the FcεRI or combination of Ca2+ ionophore and phorbol ester. Because exocytosis involves the continuous reorganization of the actin cytoskeleton, we also repeated our screen in the presence of cytochalasin D that inhibits actin polymerization. In this paper, we report on the identification of 30 Rabs as regulators of mast cell exocytosis, the involvement of 26 of which has heretofore not been recognized. Unexpectedly, these Rabs regulated exocytosis in a stimulus-dependent fashion, unless the actin skeleton was disrupted. Functional clustering of the identified Rabs suggested their classification as Rabs involved in SGs biogenesis or Rabs that control late steps of exocytosis. The latter could be further divided into Rabs that localize to the SGs and Rabs that regulate transport from the endocytic recycling compartment. Taken together, these findings unveil the Rab networks that control mast cell exocytosis and provide novel insights into their mechanisms of action.

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“…Rab8 has previously been found to act together with Rab10 [18], and we report that this second small GTPase is also involved in regulating the release of VWF. Exactly how, and through which effectors these two regulatory proteins act is now an open question, but the lack of any additive effect of the double Rab10/Rab8 knockdown indicates that they are likely .…”

Section: Discussionmentioning

confidence: 87%

“…Given the similar localization and role of Rab8A and Rab10, together with reports of these Rabs acting together or in parallel to control polarized secretion [18], we decided to analyze its localization within HUVECs. Expression of a GFPRab8A construct shows a very similar distribution within HUVECs to that of Rab10, associating with the Golgi apparatus and not colocalizing with budded WPB (Fig.…”

Section: Rab10 Function In Human Endothelial Cellsmentioning

confidence: 99%

“…Rab10, together with Rab8 and Rab13, is a member of a subfamily of Rabs similar in sequence to Sec4p, shown in yeast to be essential for postGolgi trafficking and sorting [16,17]. It has been shown to localize to the trans-Golgi network [16,18], to sorting endosomes targeting cargo to the basolateral membrane [18,19], and to early endosomes [19,20]. Rab8A is reported to have both a similar location and similar role to Rab10: it localizes to the perinuclear region and recycling endosomes [21], and also to the Golgi apparatus [22,23].…”

Section: Introductionmentioning

confidence: 99%

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A role for Rab10 in von Willebrand factor release discovered by an AP‐1 interactor screen in C. elegans

Michaux

Dyer

Nightingale

et al. 2011

Journal of Thrombosis and Haemostasis

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Summary. Background: Endothelial von Willebrand factor (VWF) mediates platelet adhesion and acts as a protective chaperone to clotting factor VIII. Rapid release of highly multimerized VWF is particularly effective in promoting hemostasis. To produce this protein, an elaborate biogenesis is required, culminating at the trans-Golgi network (TGN) in storage within secretory granules called Weibel-Palade bodies (WPB). Failure to correctly form these organelles can lead to uncontrolled secretion of low-molecular-weight multimers of VWF. The TGN-associated adaptor AP-1 and its interactors clathrin, aftiphilin and c-synergin are essential to initial WPB formation at the Golgi apparatus, and thus to VWF storage and secretion. Objectives: To identify new proteins implicated in VWF storage and/or secretion. Methods: A genomewide RNA interference (RNAi) screen was performed in the Nematode C. elegans to identify new AP-1 genetic interactors. Results: The small GTPase Rab10 was found to genetically interact with a partial loss of function of AP-1 in C. elegans. We investigated Rab10 in human primary umbilical vein endothelial cells (HUVECs). We report that Rab10 is enriched at the Golgi apparatus, where WPB are formed, and that in cells where Rab10 expression has been suppressed by siRNA, VWF secretion is altered: the amount of rapidly released VWF was significantly reduced. We also found that Rab8A has a similar function. Conclusion: Rab10 and Rab8A are new cytoplasmic factors implicated in WPB biogenesis that play a role in generating granules that can rapidly respond to secretagogue.

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Rab10 is Involved in Basolateral Transport in Polarized Madin–Darby Canine Kidney Cells

Cited by 114 publications

References 46 publications

Regulation of membrane trafficking in polarized epithelial cells

Regulation of membrane trafficking in polarized epithelial cells

Decoding the Regulation of Mast Cell Exocytosis by Networks of Rab GTPases

A role for Rab10 in von Willebrand factor release discovered by an AP‐1 interactor screen in C. elegans

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