Rab11 is required for neurite pruning and developmental membrane protein degradation in Drosophila sensory neurons

Krämer, Rafael; Rode, Sandra; Rumpf, Sebastian

doi:10.1016/j.ydbio.2019.03.003

Cited by 27 publications

(33 citation statements)

References 46 publications

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“…The degradation of cell adhesion molecule Nrg is impaired in Rab11 mutant C4da neurons during dendrite pruning [25], suggesting the requirement of Rab11 for normal membrane protein degradation. Since the small GTPase Rab family proteins are the key regulators of Proposed model for the roles of Rab11 in dendrite pruning.…”

Section: Discussionmentioning

confidence: 99%

“…While performing Rab11 RNAi in neurons, we noticed that Rab11 RNAi also caused abnormal dendritic morphology with reduced dendritic branches in larval C4da neurons ( Fig 2B) [25], compared to the control ones with Luciferase RNAi (Fig 2A). The similar phenotypes with decreasing dendritic branches were observed not only in the larval neurons with Rab11-DN expression ( Fig 2C), but also in the ones of Since Rab11 has a role in dendrite development, it is possible that the pruning defects we observed in pupal Rab11 mutant neurons are indirectly due to the abnormal dendrite morphogenesis in larval neurons, not due to the lack of Rab11 function in pupal neurons during pruning.…”

Section: Rab11 Gtpases Play Roles In Both the Dendrite Morphogenesis mentioning

confidence: 92%

“…To test this possibility, we expressed Rab11 double-stranded RNAs (dsRNAs) under the control of C4da-specific ppk-GAL4 to knockdown the endogenous Rab11 protein expression in cells by RNA interference (RNAi). We observed pruning defects in Rab11 RNAi neurons, whose primary dendrites were found connected to the soma, at 16 h APF (S2B-S2D Fig) [25], while the wild-type neurons had pruned their dendrites (S2A, S2C and S2D Fig). To rule out the nonspecific effects of UAS-Rab11-dsRNAs line in dendrite pruning, we examined the pruning phenotypes in C4da neurons of UAS-Rab11-dsRNAs line alone, without the driver ppk-GAL4, and did not observe pruning defects (S2C and S2D Fig).…”

Section: The Small Gtpase Rab11 Is Required For Dendrite Pruningmentioning

confidence: 93%

“…To further verify the requirement of Rab11 in C4da neurons during dendrite pruning, a null allele of Rab11, Rab11 ΔFRT [26], was used in mosaic analysis with a repressive cell marker (MARCM) technique to generate homozygous Rab11 mutant clones in otherwise heterozygous background by mitotic recombination [27]. The primary dendrites of C4da neurons were Fig 1E) [25], but not to the ones of control clones ( Fig 1D), at 16 h APF. Furthermore, these pruning defects could be successfully rescued by expressing the wild-type (wt) Rab11 in the C4da neurons of Rab11 ΔFRT MARCM clones ( Fig 1F, 1I and 1J), indicating that the impaired pruning is indeed caused by a loss-of-function mutation in Rab11.…”

Section: The Small Gtpase Rab11 Is Required For Dendrite Pruningmentioning

confidence: 99%

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Rab11 activation by Ik2 kinase is required for dendrite pruning in Drosophila sensory neurons

et al. 2020

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Neuronal pruning is a commonly observed phenomenon for the developing nervous systems to ensure precise wiring of neural circuits. The function of Ik2 kinase and its downstream mediator, Spindle-F (Spn-F), are essential for dendrite pruning of Drosophila sensory neurons during development. However, little is known about how Ik2/Spn-F signaling is transduced in neurons and ultimately results in dendrite pruning. Our genetic analyses and rescue experiments demonstrated that the small GTPase Rab11, especially the active GTP-bound form, is required for dendrite pruning. We also found that Rab11 shows genetic interactions with spn-F and ik2 on pruning. Live imaging of single neurons and antibody staining reveal normal Ik2 kinase activation in Rab11 mutant neurons, suggesting that Rab11 could have a functional connection downstream of and/or parallel to the Ik2 kinase signaling. Moreover, we provide biochemical evidence that both the Ik2 kinase activity and the formation of Ik2/Spn-F/Rab11 complexes are central to promote Rab11 activation in cells. Together, our studies reveal that a critical role of Ik2/Spn-F signaling in neuronal pruning is to promote Rab11 activation, which is crucial for dendrite pruning in neurons.

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Section: Discussionmentioning

confidence: 99%

Section: Rab11 Gtpases Play Roles In Both the Dendrite Morphogenesis mentioning

confidence: 92%

Section: The Small Gtpase Rab11 Is Required For Dendrite Pruningmentioning

confidence: 93%

Section: The Small Gtpase Rab11 Is Required For Dendrite Pruningmentioning

confidence: 99%

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Rab11 activation by Ik2 kinase is required for dendrite pruning in Drosophila sensory neurons

et al. 2020

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“…For instances, rab11 was reported to be a regulator of presynaptic function and axon regeneration [19,20], Rab11dependent recycling pathway was revealed to be involved in regulating cortical neuronal migration via the regulation of N-cadherin trafficking [21,22]. in Drosophila, rab11 was found to regulate dendrite pruning of sensory neurons via regulating degradation of Neuroglian [23].…”

Section: Discussionmentioning

confidence: 99%

Expression analysis of Rab11 during zebrafish embryonic development

Zhang

Gao

Qian³

et al. 2019

Preprint

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Background: Rab proteins are GTPases responsible for intracellular vesicular trafficking regulation. Rab11 proteins, members of the Rab GTPase family, are known to regulate vesicular recycling during embryonic development. In zebrafish, there are 3 rab11 paralogues, known as rab11a, rab11ba and rab11bb, sharing high identity with each other. However, the expression analysis of rab11 is so far lacking. Results: Here, by phylogeny analysis, we found the three rab11 genes are highly conserved especially for their GTPase domains. We examined the expression patterns of rab11a, rab11ba and rab11bb using RT-PCR and in situ hybridization. We found that all the three genes were highly enriched in the central nervous system, but in different areas of the brain. Apart from brain, rab11a was also expressed in caudal vein, pronephric duct, proctodeum, pharyngeal arches and digestive duct, rab11ba was detected to express in muscle, and rab11bb was expressed in kidney, fin and spinal cord. Different from rab11a and rab11ba, which both have maternal expressions in embryos, rab11bb only expresses during 24hpf to 96hpf. Conclusions: Our results suggest that rab11 genes play important but distinct roles in the development of the nervous system in zebrafish. The findings could provide new evidences for better understanding the functions of rab11 in the development of zebrafish embryos.

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Rab11 is required for neurite pruning and developmental membrane protein degradation in Drosophila sensory neurons

Cited by 27 publications

References 46 publications

Rab11 activation by Ik2 kinase is required for dendrite pruning in Drosophila sensory neurons

Rab11 activation by Ik2 kinase is required for dendrite pruning in Drosophila sensory neurons

Expression analysis of Rab11 during zebrafish embryonic development

AMPK adapts metabolism to developmental energy requirement during dendrite pruning in Drosophila

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