Rab3D, a small GTP-binding protein implicated in regulated secretion, is associated with the transcytotic pathway in rat hepatocytes

Larkin, Janet M.; Woo, Bonnie; Balan, Vijayan; Marks, David L.; Oswald, Barbara; LaRusso, Nicholas F.; McNiven, Mark A.

doi:10.1053/jhep.2000.9110

Cited by 34 publications

(24 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different combinations of the apical proteins all showed overlapping staining patterns (our unpublished data), thus the apical markers in nonpolarized cells were also used interchangeably throughout the study. Finally, rab3D, a GTPase peripherally associated with the hepatic apical PM (Larkin et al, 2000), was also present in the intracellular compartment (Figure 2, j-l).…”

Section: Apical Proteins Are Present At Pm and In A Novel Compartmentmentioning

confidence: 96%

Nonpolarized Cells Selectively Sort Apical Proteins from Cell Surface to a Novel Compartment, but Lack Apical Retention Mechanisms

Tuma

Nyasae

Hubbard

2002

MBoC

View full text Add to dashboard Cite

Membrane trafficking is central to establishing and maintaining epithelial cell polarity. One open question is to what extent the mechanisms regulating membrane trafficking are conserved between nonpolarized and polarized cells. To answer this question, we examined the dynamics of domain-specific plasma membrane (PM) proteins in three classes of hepatic cells: polarized and differentiated WIF-B cells, nonpolarized and differentiated Fao cells, and nonpolarized and nondifferentiated Clone 9 cells. In nonpolarized cells, mature apical proteins were uniformly distributed in the PM. Surprisingly, they were also in an intracellular compartment. Double labeling revealed that the compartment contained only apical proteins. By monitoring the dynamics of antibody-labeled molecules in nonpolarized cells, we further found that apical proteins rapidly recycled between the compartment and PM. In contrast, the apical PM residents in polarized cells showed neither internalization nor return to the basolateral PM from which they had originally come. Cytochalasin D treatment of these polarized cells revealed that the retention mechanisms are actin dependent. We conclude from these data that both polarized and nonpolarized cells selectively sort apical proteins from the PM and transport them to specific, but different cellular locations. We propose that the intracellular recycling compartment in nonpolarized cells is an intermediate in apical surface formation.

show abstract

Section: Apical Proteins Are Present At Pm and In A Novel Compartmentmentioning

confidence: 96%

Nonpolarized Cells Selectively Sort Apical Proteins from Cell Surface to a Novel Compartment, but Lack Apical Retention Mechanisms

Tuma

Nyasae

Hubbard

2002

MBoC

View full text Add to dashboard Cite

show abstract

“…Rab3b regulates transcytotic trafficking of pIgR from the ARE. Similarly, Rab3d localizes near the apical plasma membrane in liver cells and colocalizes with the pIgR in transcytotic carrier vesicles, which suggests it is involved in the regulation of a distal step in transcytosis in these cells (Larkin et al, 2000).…”

Section: Rab Proteinsmentioning

confidence: 96%

The subapical compartment: a traffic center in membrane polarity development

Hoekstra

Tyteca

IJzendoorn

2004

Journal of Cell Science

131

132

View full text Add to dashboard Cite

IntroductionPolarity is a ubiquitous feature of the biology of eukaryotic cells and entails the establishment and maintenance of spatial and functional asymmetry, triggered by cues from the external and intracellular environments. For example, cell movement in response to chemoattractants causes asymmetric shape changes, generating a leading edge that protrudes at the front of the cell and a rear edge that retracts (Lauffenburger and Horwitz, 1996). Instrumental in this is the establishment of membrane polarity. Molecules such as (glyco)sphingolipids segregate to the two opposing membrane surfaces (GomezMouton et al., 2001) and, by virtue of their ability to organize microdomains, may establish a platform for recruitment of the different molecular machineries required at these locations. Polarity is also apparent in neuronal cells, in which membrane domains are segregated by a diffusion barrier formed by a row of protein 'pickets' assembled on a membrane-cytoskeletal meshwork at the axonal initial segment (Nakada et al., 2003). However, most attention has focused on epithelial cells and it is these that perhaps best exemplify the principles of cell polarity, displaying asymmetric cellular morphology, molecular composition and functional properties.Epithelial cells line many tissues and simultaneously face different environments: the lumen on one side, and adjacent cells and underlying connective tissue on the other. Accordingly, compartmentalization of the cell surface and hence the operation of distinctly localized sorting and retention mechanisms are crucial for their function. Signals triggered by extracellular matrix and cell-cell cues, and relayed by key players such as integrins and E-cadherin-catenin complexes, respectively, cause the establishment of specific intercellular junctions, rearrange the cytoskeletal architecture, and activate sorting mechanisms to generate the so-called apical and basolateral plasma membrane domains (Knust, 2002;Mostov et al., 2003;Nelson, 2003). Tight junctions separate these membrane domains, preventing mixing of membrane proteins and (outer leaflet) lipids between them. Complex signaling and sorting machineries regulate transport to and from either domain. In addition, the apical and basolateral domains communicate by transcytosis, the extent of which depends on the cell type. Here, we briefly summarize what is known about these mechanisms and focus on the role of the subapical compartment (SAC), a subcellular trafficking center that is thought to orchestrate these complex trafficking pathways and is required for the generation and maintenance of epithelial membrane polarity. Endocytosis and polarity: organization and pathwaysPolarization of epithelial Madin-Darby canine kidney (MDCK) cells establishes separate apical and basolateral membrane domains, but also results in a sixfold increase in the surface area of the lateral membrane (Vega-Salas et al., 1987). This demands high levels of membrane biogenesis, which relies on a variety of closely interconnected signaling, bios...

show abstract

“…SDS-PAGE was performed in 5% to 10% gradient gels (22 cm ϫ 23 cm ϫ 1.5 mm) 13 or on 7.5% and 12% minigels, 18 which were electrophoresed at 150 to 200 V for approximately 1 hour. Some gels were processed for fluorography 16 or sandwich autoradiography to detect [ 35 S]-and [ 32 P]-labeled proteins separately.…”

Section: Methodsmentioning

confidence: 99%

“…Immunoblot analysis of hepatic proteins was performed as reported. 13 For comparison within each fluorogram or blot, the OD of the control band was set to 1.0 and the OD for the BDL band was calculated as a multiple of the control band (i.e., OD BDL :OD control ). Mean signal ratio Ϯ SD is reported when 3 or more independent blots had been performed.…”

Section: Methodsmentioning

confidence: 99%

“…Rab proteins of the Ras superfamily of guanosine triphosphate (GTP) binding proteins have been identified as molecular regulators of vesicle-mediated trafficking in various cells. 12 Our own work on rab3D 13 as well as that of other investigators on rabs 3B, 11, 13, 17, and 25, have localized these GTPases to the apical pole of hepatocytes, 14 implicating them as possible transcytotic regulators.…”

mentioning

confidence: 89%

See 1 more Smart Citation

Intracellular Accumulation of Piga–R and Regulators of Transcytotic Trafficking in Cholestatic Rat Hepatocytes

et al. 2003

View full text Add to dashboard Cite

Rab3D, a small GTP-binding protein implicated in regulated secretion, is associated with the transcytotic pathway in rat hepatocytes

Cited by 34 publications

References 39 publications

Nonpolarized Cells Selectively Sort Apical Proteins from Cell Surface to a Novel Compartment, but Lack Apical Retention Mechanisms

Nonpolarized Cells Selectively Sort Apical Proteins from Cell Surface to a Novel Compartment, but Lack Apical Retention Mechanisms

The subapical compartment: a traffic center in membrane polarity development

Intracellular Accumulation of Piga–R and Regulators of Transcytotic Trafficking in Cholestatic Rat Hepatocytes

Contact Info

Product

Resources

About