Rabbit ductus arteriosus during development: Anatomical structure and smooth muscle cell composition

Giuriato, Luca; Scatena, Marta; Chiavegato, Angela; Guidolin, Diego; Pauletto, Paolo; Sartore, Saverio

doi:10.1002/ar.1092350110

Cited by 19 publications

(17 citation statements)

References 35 publications

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“…Human DA studies have found that there is a positive correlation between endothelial cell detachment from the IEL and smooth muscle cell migration into the neointimal zone once intimal cushion formation has occurred (De Reeder et al, 1990). Yet DA intimal thickening is not essential for DA closure in all species, including smaller mammals such as rat (Jarkovska et al, 1989), rabbit (Giuriato et al, 1993), and mouse (Tada and Kishimoto, 1990). A similar lack of intimal thickening before the closure of the hatching chicken DA was observed (Bergwerff et al, 1996).…”

Section: Morphological Changesmentioning

confidence: 85%

Morphological Changes in the Chicken Ductus Arteriosi During Closure at Hatching

Belanger¹,

Copeland²,

Muirhead³

et al. 2008

The Anatomical Record

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The chicken embryo has two functioning ductus arteriosi (DA) during development. These blood vessels connect the pulmonary arteries to the descending aorta providing a right-to-left shunt of blood away from the nonrespiring lungs and to the systemic circuit and chorioallanotic membrane. The DA consists of two distinct tissue types along its length, a muscular proximal portion and an elastic distal portion. During hatching, the DA must close for proper separation of systemic and pulmonary circulation. We examined the morphological changes of the chicken DA before, during, and after hatching. Occlusion of the proximal DA began during external pipping and was complete at hatching. Anatomical remodeling began as early as external pipping with fragmentation of the internal elastic lamina and smooth muscle actin appearing in the neointimal zone. By day 2 posthatch, the proximal DA lumen was fully occluded by endothelial cells and smooth muscle actin positive cells. In contrast, the distal DA was not fully occluded by day 2 posthatch. Increases in Po 2 of the blood serves as the main stimulus for closure of the mammalian DA. The responsiveness of the chicken proximal DA to oxygen increased during hatching, peaking during external pipping. This peak correlated with an increase in blood gas Po 2 and the initial occlusion of the vessel. The distal portion remained unresponsive to oxygen throughout hatching. In conclusion, the chicken DA begins to close during external pipping when arterial Po 2 increases and vessel tone is most sensitive to oxygen.

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Section: Morphological Changesmentioning

confidence: 85%

Morphological Changes in the Chicken Ductus Arteriosi During Closure at Hatching

Belanger¹,

Copeland²,

Muirhead³

et al. 2008

The Anatomical Record

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“…Similarly, higher contractile effects of cathecolamines have been observed in the pulmonary end of human DA (48). In the majority of mammals, normal DA closure takes place from the pulmonary artery to the aortic end (17,18), but the presence of areas with so marked differences in reactivity has not been described. Development of a specific phenotype compared with surrounding arteries provides the structural and biomechanical basis for DA constriction after birth (36).…”

Section: Heterogeneity Of Contractile Responses Along the Chicken Damentioning

confidence: 97%

Ontogeny of chicken ductus arteriosus response to oxygen and vasoconstrictors

Ågren¹,

Cogolludo²,

Kessels³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

108

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The present study aimed to characterize the contractile reactivity of the chicken ductus arteriosus (DA) from the last stage of prenatal development and throughout the perinatal period. Isolated DA rings from 15-day, noninternally-pipped 19-day, and externally-pipped 21-day embryos were studied using myograph techniques. On embryonic day 15, the chicken DA did not respond to O 2 (0 to 21%), norepinephrine (NE), or phenylephrine (Phe) but contracted in response to high-K ϩ solution, the inhibitor of voltage-gated channels 4-aminopyridine, U-46619, and endothelin (ET)-1. These responses increased with advancing incubation age. Contractile responses to O 2, NE, and Phe were present in the 19-and 21-day embryo. Oxygen-induced contraction was restricted to the pulmonary side of the DA and was augmented by the nitric oxide synthase inhibitor N -nitro-L-arginine methyl ester and the soluble guanylate cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one and reduced by the peptidic ET A and ETB-receptor antagonist PD-142,893. Transmural electrical stimulation of nerves, the nonselective cyclooxygenase (COX) inhibitor indomethacin, the COX-1 inhibitor valeryl salicylate, the COX-2 inhibitor nimesulide, the inhibitor of ATP-sensitive K ϩ channels glibenclamide, and the inhibitor of Ca 2ϩ -activated K ϩ channels tetraethylammonium did not cause contraction of the DA rings at any age. We conclude that transition to ex ovo life is accompanied by dramatic changes in chicken DA reactivity. At 0.7 incubation, excitation-contraction and pharmacomechanical coupling for several contractile agonists are already present, whereas the constrictor effects of O 2 and cathecolamines appear later in development and are located in the pulmonary side of the DA. ductus arteriosus; chicken embryo; potassium channels; oxygen sensing; cathecolamines.THE DUCTUS ARTERIOSUS (DA) is a vessel that connects the pulmonary artery to the aorta and provides, during the fetal life, a pulmonary-to-systemic diversion that shunts more than half of the right ventricle output away from the nonventilated lungs into the systemic circulation (43, 47). The main factors maintaining patency of the DA in utero are low O 2 tension, high levels of circulating PGE 2 , and locally produced PGE 2 and PGI 2 (24, 43). Failure of DA closure after birth is a common complication of premature delivery that is still presenting challenges in terms of diagnosis, assessment, and treatment options (43).Although the isolated DA is sensitive to a wide range of contractile agonists, the major factor actively stimulating contraction at birth is increasing O 2 tension, which has a profound effect on the DA, both directly and by modulating its response to vasodilators and vasoconstrictors (43). To constrict properly after birth, the DA prepares itself for this specific task from a quite early onset during development (3). This preparation is reflected by changes in responsiveness with advancing gestational age. These have been extensively characterized in numerous mammalian specie...

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“…Frozen specimens of the aortic arch (about 5 mm long) from 60-day-old WHHL or aged-matched control NZW rabbits 29 were serially sectioned, in a transverse plane, at 20-/nm intervals. Cryosections were fixed for 10 minutes in 10% formalin buffered in PBS and then processed for hematoxylin-eosin staining.…”

Section: Histologymentioning

confidence: 99%

“…In fact, in this vascular region of 60-day-old WHHL rabbits, a grossly visible, raised lesion was consistently found (Figure 1). This lesion was localized at the level of a small cavity in the arch, which is formed during the closure of ductus arteriosus 29 ( Figure 1). Light microscopy analysis of hematoxylin-eosin-stained cryosections from this vascular region confirmed that the lesion was localized at the level of the juncture of the ligamentum arteriosum with the aortic wall (not shown).…”

Section: Localization Of Vascular Wall Changes In Whhl Rabbitsmentioning

confidence: 99%

Localization and smooth muscle cell composition of atherosclerotic lesions in Watanabe heritable hyperlipidemic rabbits.

Giuriato

Scatena

Chiavegato

et al. 1993

Arterioscler Thromb

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Morphological techniques (histology and electron microscopy), as well as immunofluorescence assays, were applied to the study of the localization and smooth muscle cell (SMC) composition of atherosclerotic lesions in Watanabe heritable hyperlipidemic (WHHL) rabbits during a 4.5-month period. Vascular segments from different arteries (carotid, coronary, and iliac arteries) or from the same vessel at different levels (aorta) of animals at days 7,15, 30, 40, 60, 90, and 135 showed that the atherosclerotic lesion first became visible at the level of the aortic arch in 60-day-old WHHL animals. Histological examination of serial cryosections from this vascular region indicated that the vascular lesion arose from a cavity in the media layer, located anatomically at the level of the juncture of the ligamentum arteriosum with the aortic arch. This aortic arch cavity is formed during the postnatal closure of the ductus arteriosus and is characterized by the presence of a thickened intima, which was absent in the other vascular regions examined. Immunofluorescence comparison of normal and atherosclerotic tissues from the aortic arch cavity wall with the use of monoclonal antibodies specific for smooth muscle and nonmuscle myosin isoforms revealed the existence of distinct SMC populations. SMCs in the thickened intima showed a myosin isoform pattern peculiar to cells with a degree of maturation intermediate between the fully differentiated and the developing (fetal) aortic SMCs. By contrast, SMCs present in atherosclerotic lesions displayed a predominant fetal-type pattern of myosin isoform expression. The achievement of this myosin isoform content seems to be correlated with the accumulation of lipids in the intima. In the media subjacent to the intimal thickening or atherosclerotic lesion, SMCs primarily displayed an intermediate degree of maturation. In older WHHL animals and at this aortic level, the SMC composition of the atherosclerotic lesion did not change, whereas in the subjacent media, the cells of intermediate type almost disappeared. In the vascular regions in which the atherosclerotic lesion appeared at later stages, such as near the aortic bifurcation, the distribution of fetal and intermediate cell types in the atherosclerotic wall was similar to that taken at the aortic arch level. These results indicate that there is 1) a preferential anatomic site from which atherogenesis initiates in WHHL rabbits; 2) a time correlation between the accumulation of lipids in the wall and the phenotypic change of SMCs toward a poorly differentiated cell type; and 3) the tendency for SMCs to follow the same differentiation pattern in early atherosclerotic lesions, irrespective of the site and time at which they develop. (

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Rabbit ductus arteriosus during development: Anatomical structure and smooth muscle cell composition

Cited by 19 publications

References 35 publications

Morphological Changes in the Chicken Ductus Arteriosi During Closure at Hatching

Morphological Changes in the Chicken Ductus Arteriosi During Closure at Hatching

Ontogeny of chicken ductus arteriosus response to oxygen and vasoconstrictors

Localization and smooth muscle cell composition of atherosclerotic lesions in Watanabe heritable hyperlipidemic rabbits.

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