Rabbit erythrocyte survival following diminished sialic acid and ATP depletion

Marikovsky, Y.; Elazar, E.; Danon, D.

doi:10.1016/0047-6374(77)90024-0

Cited by 27 publications

(7 citation statements)

References 13 publications

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“…Liver and spleen sequester desialylated RBCs. 78,79 The human platelet lifespan is about 7 to 10 days, whereas transfused platelet lifespan is only 4 to 5 days. 80,81 The initial clearance of a portion of transfused platelets occurs, as for RBCs, within the first 24 hours, primarily in the spleen and liver and de- 86 The mechanisms behind the initial clearance of transfused platelets defining platelet recovery remain elusive but are independent of apoptosis and change in glycosylation.…”

Section: Recovery and Lifespan Of Transfused Platelets And Rbcsmentioning

confidence: 99%

Multifaceted role of glycosylation in transfusion medicine, platelets, and red blood cells

Lee‐Sundlov

Stowell

Hoffmeister

2020

Journal of Thrombosis and Haemostasis

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The conjugation of carbohydrate moieties, or saccharides, onto various glycosyl acceptors, be it a protein, lipid, or any organic molecule, defines glycosylation. It is a very prevalent posttranslational nontemplated modification. Glycosylation is highly modular; carbohydrate building blocks are repeatedly linked and assembled in varying lengths, branches, and configurations. Being nontemplated, glycosylation does not have canonical patterns, which can result in high diversity and an extensive repertoire of biologically functional molecules.Despite their seemingly nontemplated assembly, glycans synthesis does appear to adhere to some common structural patterns. Humans use nine monosaccharides in various glycoconjugate forms (Table 1).Glycan residues are conjugated on asparagine (N-glycan) or serine/ threonine (O-glycans) residues (Figure 1) to form glycoproteins. Two N-acetylglucosamine (GlcNAc) and three mannose residues often serves as the core of N-glycans, which are often highly branched.Conversely, O-glycans are linked with N-acetylgalactosamine (GalNAc) moiety and are often less branched than N-glycans. Although N-glycan attachment to proteins occurs on asparagines (N) within the consensus (N glycan sequon) NXS/T, where X is any amino acid except proline, there is no predictive sequon for O-glycans. Rather, O-glycans often occur in "bottle brush" conformations in variable

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Section: Recovery and Lifespan Of Transfused Platelets And Rbcsmentioning

confidence: 99%

Multifaceted role of glycosylation in transfusion medicine, platelets, and red blood cells

Lee‐Sundlov

Stowell

Hoffmeister

2020

Journal of Thrombosis and Haemostasis

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“…In addition to the decrease in membrane SA seen in aged cells, when the membrane SA of RBCs is reduced experimentally by enzyme treatment, the biochemical and biophysical properties and survival time of the cells also suffer . Similarly, since the effects of neuraminidases, significant fall in SA was noted as well on the RBCs of the patients with diseases such as diabetes mellitus, malaria, anaemia and inflammation like in sepsis .…”

Section: Introductionmentioning

confidence: 99%

Restoring the youth of aged red blood cells and extending their lifespan in circulation by remodelling membrane sialic acid

Huang¹,

Tuo²,

Wang³

et al. 2015

J Cellular Molecular Medi

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Membrane sialic acid (SA) plays an important role in the survival of red blood cells (RBCs), the age‐related reduction in SA content negatively impacts both the structure and function of these cells. We have therefore suggested that remodelling the SA in the membrane of aged cells would help recover cellular functions characteristic of young RBCs. We developed an effective method for the re‐sialylation of aged RBCs by which the cells were incubated with SA in the presence of cytidine triphosphate (CTP) and α‐2,3‐sialytransferase. We found that RBCs could be re‐sialylated if they had available SA‐binding groups and after the re‐sialylation, aged RBCs could restore their membrane SA to the level in young RBCs. Once the membrane SA was restored, the aged RBCs showed recovery of their biophysical and biochemical properties to similar levels as in young RBCs. Their life span in circulation was also extended to twofold. Our findings indicate that remodelling membrane SA not only helps restore the youth of aged RBCs, but also helps recover injured RBCs.

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“…Hence, there has been a recurrent suspicion that decreased ATP concentration might be involved 1n destruction of senescent red cells, despite some contrary evidence (7,8).…”

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confidence: 99%