Rabbit kidney proximal tubule cells in primary culture: Evaluation of the impact of expressed phenotype on cellular toxic response

Morin, Jean Paul; Monteil, Christelle; Glomot, R.; Fillastre, J P

doi:10.1016/0887-2333(91)90058-l

Cited by 7 publications

(3 citation statements)

References 23 publications

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“…Metabolism of RPTC in primary culture rapidly becomes predominantly glycolytic (Chung et aI., 1982;Sakhrani et aI., 1984;Tang et aI., 1989, Toutain et aI., 1991. As discussed in the literature, three main factors have been claimed to be responsible for this metabolic orientation : i) availability of metabolism substrates (Tang and Tannen, 1990;Morin et al, 1991, Blais et aI., 1992Aleo andSchnellmann, 1992, Jung et aI., 1992), ii) cellular proliferation (Tang et aI., 1989) or iii) limitation of O 2 availability (Aleo and Schnellmann, 1992). Previous studies from our laboratory indicated that insulin and glucose deprivation in the culture medium only partially prevented the rise in glycolytic activity and delayed the drop of gluconeogenic pathways (Morin et aI., 1991;Monteil et aI., 1993).…”

Section: Discussionmentioning

confidence: 99%

Modulation of glycolysis induction in primary cultures of rabbit kidney proximal tubule cells: the role of shaking, glucose and insulin.

Monteil

Leclere

Dantzer

et al. 1993

Cell Biology International

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We have assessed the impact of increasing oxygen availability on cellular phenotype expression of rabbit proximal tubule cells in primary culture developed with variable glucose and/or insulin contents. To mitigate hypoxia at the cell/medium interface, cells were shaken for the whole culture duration and their expressed phenotype was compared with those expressed by static cultures. O2 and CO2 tensions were kept constant in the incubator atmosphere. Glycolysis and gluconeogenesis pathways, detoxication system, and mitochondrial, apical and basolateral membrane marker enzyme activities were assessed. This study showed that the induction of glycolysis which appear in primary cultures of proximal tubule cells may be partially prevented by continuously shaking the cultures. This effect was more marked in the presence of glucose, suggesting better substrate oxidation in shaken cultures.

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Section: Discussionmentioning

confidence: 99%

Modulation of glycolysis induction in primary cultures of rabbit kidney proximal tubule cells: the role of shaking, glucose and insulin.

Monteil

Leclere

Dantzer

et al. 1993

Cell Biology International

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“…The effects of high glucose on transport by primary RPT cells have been examined so as to understand the changes that occur in the RPT during diabetes [ 82 , 87 ]. The effects of toxicants have been studied extensively in primary rabbit RPT cell cultures [ 88 , 89 , 90 ], given that this nephron segment is a target of a number of drugs, oxidants, and heavy metals. Finally, primary rabbit kidney cell cultures have been the source of a number of established cell lines, including those originating from the proximal tubule [ 91 , 92 , 93 ].…”

Section: Regulation Of the Nak-atpase β1 Subunit Gene Amentioning

confidence: 99%

Gene Level Regulation of Na,K-ATPase in the Renal Proximal Tubule Is Controlled by Two Independent but Interacting Regulatory Mechanisms Involving Salt Inducible Kinase 1 and CREB-Regulated Transcriptional Coactivators

Taub

2018

IJMS

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For many years, studies concerning the regulation of Na,K-ATPase were restricted to acute regulatory mechanisms, which affected the phosphorylation of Na,K-ATPase, and thus its retention on the plasma membrane. However, in recent years, this focus has changed. Na,K-ATPase has been established as a signal transducer, which becomes part of a signaling complex as a consequence of ouabain binding. Na,K-ATPase within this signaling complex is localized in caveolae, where Na,K-ATPase has also been observed to regulate Inositol 1,4,5-Trisphosphate Receptor (IP3R)-mediated calcium release. This latter association has been implicated as playing a role in signaling by G Protein Coupled Receptors (GPCRs). Here, the consequences of signaling by renal effectors that act via such GPCRs are reviewed, including their regulatory effects on Na,K-ATPase gene expression in the renal proximal tubule (RPT). Two major types of gene regulation entail signaling by Salt Inducible Kinase 1 (SIK1). On one hand, SIK1 acts so as to block signaling via cAMP Response Element (CRE) Binding Protein (CREB) Regulated Transcriptional Coactivators (CRTCs) and on the other hand, SIK1 acts so as to stimulate signaling via the Myocyte Enhancer Factor 2 (MEF2)/nuclear factor of activated T cell (NFAT) regulated genes. Ultimate consequences of these pathways include regulatory effects which alter the rate of transcription of the Na,K-ATPase β1 subunit gene atp1b1 by CREB, as well as by MEF2/NFAT.

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“…The possibility for cells to grow in total absence of hexoses, pentoses and ribonucleosides im plies the capacity of the cells to synthesize phosphorylated sugars from oxaloacetate, amino acids, or trioses through the gluconeo genic pathway. Proliferation of proximal tu bule cells in our model in the absence of hex oses, pentoses and ribonucleosides which was described by Morin et al [26,27] provides the evidence for an active gluconeogenic pathway in our culture condition. This is in agreement with the data of Goligorski et al [ 1] and Wang and Taub [28] who showed that in low glucose media (<5 mM) or glucose-free media proxi mal tubule cells in primary culture were capa ble of gluconeogenesis, and that these cells were able to reutilize significant amounts of the glucose produced under these conditions.…”

Section: Discussionmentioning

confidence: 84%

Insulin and Glucose Impact on Functional and Morphological Differentiation of Rabbit Proximal Tubule Cells in Primary Culture

Monteil

Leclere²,

Fillastre³

et al. 1993

Kidney Blood Press Res

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Glucose and insulin impact on cellular phenotype expression was assessed on rabbit proximal tubule cells in primary culture. Glycolysis, gluconeogenesis pathways and lysosomal, mitochondrial, detoxication system, apical and basolateral membrane marker enzyme activities were assessed. Both insulin and glucose deprivation partially prevented the rise in glycolysis and delayed the drop of gluconeogenesis pathways. Scanning electron microscopy analysis of the apical surface of the monolayer revealed a much higher density of microvilli in glucose-free cultures compared to cultures developed with glucose. In conclusion, culture medium deprivation in both glucose and insulin allowed closer functional, biochemical and morphological properties to those which exist in the in vivo situation for proximal tubule cells.

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Rabbit kidney proximal tubule cells in primary culture: Evaluation of the impact of expressed phenotype on cellular toxic response

Cited by 7 publications

References 23 publications

Modulation of glycolysis induction in primary cultures of rabbit kidney proximal tubule cells: the role of shaking, glucose and insulin.

Modulation of glycolysis induction in primary cultures of rabbit kidney proximal tubule cells: the role of shaking, glucose and insulin.

Gene Level Regulation of Na,K-ATPase in the Renal Proximal Tubule Is Controlled by Two Independent but Interacting Regulatory Mechanisms Involving Salt Inducible Kinase 1 and CREB-Regulated Transcriptional Coactivators

Insulin and Glucose Impact on Functional and Morphological Differentiation of Rabbit Proximal Tubule Cells in Primary Culture

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