Rabbit model of tracheal stenosis using cylindrical diffuser

Lee, Hyoung Shin; Kim, Sung Won; Oak, Chulho; Kang, Hyun Wook; Oh, Junghwan; Jung, Min-Jung; Kim, Sung Bin; Won, Jun Hee; Lee, Kang Dae

doi:10.1002/lsm.22615

Cited by 11 publications

(16 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It may be difficult to validate the impact of certain materials or devices to prevent tracheal stenosis based on such models. We have established a reliable animal model of tracheal stenosis that shows a predictable grade of stenosis according to the delivered energy of cylindrical diffusion laser irradiation (Lee et al, ). Therefore, we believe that the difference between rabbits of the two groups in our study may be more reliable than the results of previous studies.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, we obtained polyphenolic compounds (10 g) and stored at −80°C until use. These polyphenolic compounds referred as phlorotannins have been known to be rich in E. cava (Lee et al, ). According to the previous studies (Kang et al, ; Kim, Lee, Shin, & Lee, ; Lee et al, ), phlorotannins from E. cava are composed of rich polyphenols (45.99%) and these consist of phloroglucinol (13.91 mg), eckol (1.24 mg), 6,6‐bieckol (0.59 mg), dieckol (55.16 mg), and phlorofucofuroeckol (0.61 mg; Figure ).…”

Section: Methodsmentioning

confidence: 99%

“…In this study, we sought to evaluate the ability of phlorotannins to prevent tracheal stenosis based on both in vitro and in vivo studies. We recently established an animal model of tracheal stenosis with a reproducible grade of tracheal stenosis using diffusing laser irradiation (Lee et al, ). Using this reliable in vivo model, we evaluated the feasibility of using a segmented endotracheal tube coated with phlorotannin to ameliorate or prevent tracheal stenosis.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fabrication and biological activity of polycaprolactone/phlorotannin endotracheal tube to prevent tracheal stenosis: An in vitro and in vivo study

Lee

Jeong

et al. 2019

J Biomed Mater Res

Self Cite

View full text Add to dashboard Cite

Prolonged endotracheal intubation is the most common cause of tracheal stenosis, which may lead to serious airway obstruction. Development of an endotracheal tube coated with biomaterials that exhibit anti‐inflammatory or anti‐fibrogenic effects may prevent tracheal stenosis. This study demonstrates that an endotracheal tube coated with phlorotannin, which is present in extracts of the brown alga Ecklonia cava, can prevent tracheal stenosis in a rabbit model. An in vitro study shows that phlorotannin inhibits proliferation of human tracheal fibroblasts treated with transforming growth factor β1. Phlorotannin‐coated endotracheal tubes show steady release of phlorotannin for up to 7 days, and removal of the tube 1 week after insertion reveals a reduction in both fibrogenesis and thickening of tracheal submucosa. Western blot analysis of tracheal tissues after removal of the phlorotannin‐coated tube shows decreased protein expression levels of phenotypic markers of fibrosis such as collagen type I and α‐smooth muscle actin. The ability of phlorotannin‐coated endotracheal tube to prevent tracheal stenosis caused by endotracheal intubation indicates that phlorotannin may be considered as a candidate biomaterial for coating the cuff of endotracheal tubes to prevent tracheal stenosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fabrication and biological activity of polycaprolactone/phlorotannin endotracheal tube to prevent tracheal stenosis: An in vitro and in vivo study

Lee

Jeong

et al. 2019

J Biomed Mater Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…Other biological factors reacting with PHL and LLLT may lead to various responses, particularly in in vivo environments. To validate the safety and efficacy of the combined therapy for clinical translation, further in vivo testing is under way using animal models with skin scarring and trachea stenosis that were established in our previous studies . The chronic response of tissue fibrosis after the combined treatment will be monitored to confirm the proposed therapeutic effect and to demonstrate the principal mechanism by immunohistochemistry and cell signaling analysis.…”

Section: Discussionmentioning

confidence: 99%

Combined treatment of low‐level laser therapy and phloroglucinol for inhibition of fibrosis

et al. 2019

Self Cite

View full text Add to dashboard Cite

Background and Objectives Fibrosis is a highly prevalent disease, which is responsible for 45% of deaths through pathological effects in developed countries. Previous studies have reported that low‐level laser therapy (LLLT) can modulate fibrotic activity, but significant enhancement of therapeutic efficacy is still required for clinical translation. The aim of this study is to evaluate the feasible effect of LLLT combined with phloroglucinol (PHL) on the inhibition of fibrosis in vitro. Study Design/Materials and Methods NIH/3T3 murine embryonic fibroblasts cells were cultured and transforming growth factor‐β1 (TGF‐β1) was treated for transition of fibroblasts. After TGF‐β1 treatment, LLLT and PHL were used, respectively, and in combination to suppress fibrosis. 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide and BrdU assays were performed to estimate the cell viability and proliferation. To evaluate the expression of fibrotic markers, we used confocal immunofluorescence and western blot. Results When compared with respectively treated groups, the group with the combined treatment of LLLT and PHL significantly reduced cell viability and proliferation. Immunofluorescence staining showed that the combined group minimized more α‐smooth muscle actin (α‐SMA) and type I collagen than the other groups. Western blot analysis showed that the combined treatment had significant decreases in α‐SMA, TGF‐β1, and type I collagen. Conclusions PHL‐assisted LLLT may be an effective treatment to inhibit fibrosis due to its additive effects. The combined treatment has a potential to be an alternative treatment for fibrosis. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

show abstract

“…Recently, diffusing optical applicators have been developed and investigated for the treatment of tubular tissue such as photodynamic therapy, nasopharyngeal carcinoma treatment [5], laser lipolysis [6], endovenous laser ablation [7], and tracheal stenosis treatment [8]. However, these developments are partly finished and their availability on the market is still unsatisfactory.…”

Section: Introductionmentioning

confidence: 99%

Spatial effect of conical angle on optical-thermal distribution for circumferential photocoagulation

Truong¹,

Park²,

Tran³

et al. 2017

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

A uniformly diffusing applicator can be advantageous for laser treatment of tubular tissue. The current study investigated various conical angles for diffuser tips as a critical factor for achieving radially uniform light emission. A customized goniometer was employed to characterize the spatial uniformity of the light propagation. An model was developed to quantitatively compare the temperature development and irreversible tissue coagulation. The 10-mm diffuser tip with angle at 25° achieved a uniform longitudinal intensity profile (i.e., 0.90 ± 0.07) as well as a consistent thermal denaturation on the tissue. The proposed conical angle can be instrumental in determining the uniformity of light distribution for the photothermal treatment of tubular tissue.

show abstract

Rabbit model of tracheal stenosis using cylindrical diffuser

Abstract: The degree of stenosis was significantly different according to the delivered optical energy to tracheal mucosa. Therefore, the proposed model may be used in animal studies to emulate variable grades of tracheal stenosis. Lasers Surg. Med. 49:372-379, 2017. © 2016 Wiley Periodicals, Inc.

Cited by 11 publications

References 21 publications

Fabrication and biological activity of polycaprolactone/phlorotannin endotracheal tube to prevent tracheal stenosis: An in vitro and in vivo study

Fabrication and biological activity of polycaprolactone/phlorotannin endotracheal tube to prevent tracheal stenosis: An in vitro and in vivo study

Combined treatment of low‐level laser therapy and phloroglucinol for inhibition of fibrosis

Spatial effect of conical angle on optical-thermal distribution for circumferential photocoagulation

Contact Info

Product

Resources

About