Rabbit serum against K1 peptide, an immunogenic epitope of the Trypanosoma cruzi KMP-11, decreases parasite invasion to cells

A terpenoid-rich extract from Clethra fimbriata exhibits anti-Trypanosoma cruzi activity and induces T cell cytokine production

“…The number of infected cells was estimated by counting 200 randomly distributed cells using 100 X magnification in a light microscope. Three independent experiments were performed in duplicate [ 31 ].…”

Altering the motility of Trypanosoma cruzi with rabbit polyclonal anti-peptide antibodies reduces infection to susceptible mammalian cells

“…As we have previously described (Diaz-Soto et al, 2012), polyclonal antibodies against TcTLE peptide are able to bind to the native KMP-11 on T. cruzi's trypomastigote surface and reduce the parasite's capacity to infect susceptible Vero cells. However, the relatively low antibody titers, although significant for immunization with a 9-mer peptide alone, limited further functional analysis.…”

“…We have previously shown that immunization of rabbits with the TcTLE peptide produces polyclonal antibodies that recognize live forms of T. cruzi and reduce parasite invasion in Vero cells (Diaz-Soto et al, 2012). However, the low antibody titers obtained prevented further assessment of their potential functional properties.…”

Trypanosoma cruzi-specific CD8+ T cells and other immunological hallmarks in chronic Chagas cardiomyopathy: Two decades of research

“…%, SD = 0.07). In addition, MP-FLU-specific CD8 + T cells were predominantly effector memory T cells (T EM : CCR7 -CD62L -), distributed in the early or intermediate/late differentiation stages, producing IL-2, IFN-g, CD107a/b, and perforin without showing significant differences when compared with those from HD (Lasso et al, 2012). In all, these data support the hypothesis that parasite antigens induce a specific CD8 + T cell dysfunctionality in terms of reduced proliferation capacity and a prolonged activation state.…”

“…It was also studied whether the alteration in the functionality of CD8 + T cells was nonspecific or specific to parasite antigens; we studied the CD8 + T cell response of CCP against an antigen of another microorganism. In this case, the MP-FLU peptide (GILGFVFTL) derived from the influenza virus, which is also restricted to HLA-A2 (Lasso et al, 2012). The results showed that MP-FLU peptide-specific CD8 + T cells presented similar frequencies in the five HLA-A2 + HD (0.12-0.29%, mean 0.17%, SD = 0.07) and the 13 HLA-A2 + CCP (0.12-0.37%, mean 0.21.…”