Rabbit Study of an In Situ Forming Hydrogel Vitreous Substitute

Swindle-Reilly, Katelyn E; Shah, Milan; Hamilton, Paul D.; Eskin, Thomas A.; Kaushal, Shalesh; Ravi, Nathan

doi:10.1167/iovs.08-2891

Cited by 70 publications

(53 citation statements)

References 20 publications

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“…Several groups have developed synthetic gels to replace the vitreous after vitrectomy [86][87][88][89] . In most cases, these preparations were designed to assist in re-attaching the retina after detachment.…”

Section: Preventing Nuclear Cataractmentioning

confidence: 99%

Oxidative Damage and the Prevention of Age-Related Cataracts

Beebe

Holekamp²,

Shui³

2010

Ophthalmic Res

226

158

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Purpose: Cataracts are often considered to be an unavoidable consequence of aging. Oxidative damage is a major cause or consequence of cortical and nuclear cataracts, the most common types of age-related cataracts. Methods: In this review, we consider the different risk factors, natural history and etiology of each of the 3 major types of age-related cataract, as well as the potential sources of oxidative injury to the lens and the mechanisms that protect against these insults. The evidence linking different oxidative stresses to the different types of cataracts is critically evaluated. Results: We conclude from this analysis that the evidence for a causal role of oxidation is strong for nuclear, but substantially lower for cortical and posterior subcapsular cataracts. The preponderance of evidence suggests that exposure to increased levels of molecular oxygen accelerates the age-related opacification of the lens nucleus, leading to nuclear cataract. Factors in the eye that maintain low oxygen partial pressure around the lens are, therefore, important in protecting the lens from nuclear cataract. Conclusions: Maintaining or restoring the low oxygen partial pressure around that lens should decrease or prevent nuclear cataracts.

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Section: Preventing Nuclear Cataractmentioning

confidence: 99%

Oxidative Damage and the Prevention of Age-Related Cataracts

Beebe

Holekamp²,

Shui³

2010

Ophthalmic Res

226

158

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“…The protocol was as follows: under a dry nitrogen atmosphere, 0.5 g of macromonomer (6) was dissolved in DMF and 5 g of AMPS-Na þ was added. Additionally, DMF (20 mL in total) was added dropwise until a clear homogenous solution was obtained.…”

Section: Viscosimetric Measurementsmentioning

confidence: 99%

“…[4,5] Swindle-Reilly et al presented an in situ gelling copolymer hydrogel using disulfide crosslinks formed by air oxidation. [6] Therefore, the design of novel polymers and regarding to those in situ forming systems appears especially attractive. We propose a new concept as a practical consideration for vitreous substitutes, based on thermoresponsive in situ gelation.…”

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confidence: 99%

In Situ Forming Hydrogels: A Thermo‐Responsive Polyelectrolyte as Promising Liquid Artificial Vitreous Body Replacement

et al. 2011

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The use of fully synthetic polymers as a liquid artificial vitreous body replacement (LAVR) was already considered, but yet none of these polymers have proven to be successful in long term retention in the vitreous cavity due to inadequate biocompatibility and fast biodegradation. Our novel cross‐linked polyelectrolyte as a potential intraocular endotamponade shows an innovative concept of thermoresponsive behavior proposed for the first time in ophthalmologic surgery. The polyelectrolyte has been elaborated via a three‐step procedure by combining classical synthetic protocols and microwave technique. We observed that the polyelectrolyte comprising sodium 2‐acrylamido‐2‐methylpropane sulfonate and acrylic functionalized poly(N‐isopropylacrylamide) macromonomer with glycerol dimethacrylate as cross‐linker displays a noticeable thermosensitive behavior. It exhibits enhancement of shear viscosity at physiological temperatures without phase separation. First in vitro experiments on a retinal ganglion cell line (RGC‐5) present a promising biocompatibility without harmful effects. Cell viability, proliferation rate, and inflammatory cascade (COX‐2 and iNOS expression) were not affected.

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“…18 There is a type of hydrogel that can undergo sol-gel transformation by inducing an oxidationreduction reaction or a thermal difference, but its use is extremely inconvenient because the hydrogel must be handled in a vacuum or under a controlled temperature immediately prior to injection. 19,20 A self-assembling peptide, SPG-178, spontaneously assembles itself into nanofibers, creating a stable b-sheet structure in water. The fibers then spontaneously build themselves into a three-dimensional mesh net (Fig.…”

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confidence: 99%

A Self-Assembling Peptide Gel as a Vitreous Substitute: A Rabbit Study

Uesugi

Sakaguchi

Hayashida

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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Citation: Uesugi K, Sakaguchi H, Hayashida Y, et al. A self-assembling peptide gel as a vitreous substitute: a rabbit study. Invest Ophthalmol Vis Sci. 2017;58:4068-4075. DOI: 10.1167/iovs.17-21536 PURPOSE. To evaluate a self-assembling peptide gel as a potential vitreous substitute.METHODS. PanaceaGel SPG-178, a self-assembling peptide gel, was diluted with distilled water and a balanced salt solution to achieve a final peptide concentration of 0.1%. The gel's refractive index, visible light transmission rate, and rheologic properties were investigated. The gel's biocompatibility was evaluated by examining the cellular viability (live and dead staining) and proliferation rate (alamarBlue assay). A 25-G pars plana vitrectomy was performed on the right eye of 21 New Zealand white rabbits. The gel was then injected into the vitreous cavity of 15 eyes. Six eyes were injected with a balanced salt solution (BSS) and served as controls. Toxicity was examined using electroretinography and histologic analysis after the injection of the gel.RESULTS. The gel's physical properties closely resembled those of human vitreous. The gel showed no apparent toxicity. When the gel was injected into the vitreous cavity, fragmentation was not observed. Additionally, the gel remained transparent in the vitreous cavity and no complications were observed for 3 months after the injection. Electroretinography and histology confirmed the gel's biocompatibility.CONCLUSIONS. This diluted self-assembling peptide gel could be provide a promising vitreous substitute.Keywords: vitreous humor, retina, self-assembling peptide gel T he vitreous is located in the posterior segment of the eye and fills approximately two-thirds of the globe. It is a waterbased hydrogel containing collagen and hyaluronic acid. It also plays an important role in the pathophysiology for some vitreoretinal diseases, including retinal detachment and macular hole. 1,2To treat these diseases, the vitrectomy is an important procedure, and the use of vitreous substitute is necessary. The aim of its use is to not only provide an organic substitute to recover IOP and eyeball volume but to also supply a functional substitute for retinal reattachment. Air, expandable gas, and silicone are currently used as vitreous substitutes. However, air and expandable gas are not suitable long-term substitutes due to their absorption. A silicone substitute must be removed from the eye after a specific period of time because its toxicity can lead to complications, including glaucoma and cataracts. Because of their hydrophobic properties of these substitutes, the eye is not completely filled and growth factors can accumulate in the unfilled spaces. This may also lead to postoperative complications, including development of proliferative membranes. [4][5][6] Recently, several studies have examined both natural 7-13 and synthetic 2,14-20 hydrophilic polymers as vitreous substitutes. These polymers do not have limitations related to hydrophobicity, but other problems may be observed. Hydrogels ...

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Rabbit Study of an In Situ Forming Hydrogel Vitreous Substitute

Cited by 70 publications

References 20 publications

Oxidative Damage and the Prevention of Age-Related Cataracts

Oxidative Damage and the Prevention of Age-Related Cataracts

In Situ Forming Hydrogels: A Thermo‐Responsive Polyelectrolyte as Promising Liquid Artificial Vitreous Body Replacement

A Self-Assembling Peptide Gel as a Vitreous Substitute: A Rabbit Study

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