Rabbit VX2 Liver Tumor Model: A Review of Clinical, Biology, Histology, and Tumor Microenvironment Characteristics

Pascale, Florentina; Pelage, Jean-Pierre; Wassef, Michel; Ghegediban, S.; Saint‐Maurice, Jean‐Pierre; Baère, Thierry De; Denys, Alban; Durán, Rafael; Deschamps, Frédèric; Pellerin, Olivier; Maeda, Nobuji; Laurent, Alexandre; Namur, Julien

doi:10.3389/fonc.2022.871829

Cited by 10 publications

(5 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This tumor can be transplanted serially from one animal to another by means of xenotransplantation and can grow in any organ or grafted tissue. A notable feature of VX2 is that it does not require genetically modified or immunocompromised subjects but can be transplanted into immunocompetent animals [32]. We observed morphological differences in VX2 tumor cells across multiple focal planes and documented the biological process of cell division in situ over a period of 1 hour, demonstrating the ability of our ultra-low-cost smartphone microscope to have real-time microscopic monitoring of closed cell culture processes.…”

Section: Application In the Monitoring Of Vx2 Tumor Cell Culturementioning

confidence: 80%

Towards ultra-low-cost smartphone microscopy

Zhang,

Zuo

et al. 2023

Preprint

View full text Add to dashboard Cite

The outbreak of COVID-19 exposed the inadequacy of our technical tools for home health surveillance, and recent studies have shown the potential of smartphones as a universal optical microscopic imaging platform for such applications. However, most of them use laboratory-grade optomechanical components and transmitted illuminations to ensure focus tuning capability and imaging quality, which keeps the cost of the equipment high. Here we propose an ultra-low-cost solution for smartphone microscopy. To realize focus tunability, we designed a seesaw-like structure capable of converting large displacements on one side into small displacements on the other (reduced to ∼9.1%), which leverages the intrinsic flexibility of 3D printing materials. We achieved a focus-tuning accuracy of ∼ 5µm, which is 40 times higher than the machining accuracy of the 3D-printed lens holder itself. For microscopic imaging, we use an off-the-shelf smartphone camera lens as the objective and the built-in flashlight as the illumination. To compensate for the resulting image quality degradation, we developed a learning-based image enhancement method. We use the CycleGAN architecture to establish the mapping from smartphone microscope images to benchtop microscope images without pairing. We verified the imaging performance on different biomedical samples. Except for the smartphone, we kept the full costs of the device under 4 USD. We think these efforts to lower the costs of smartphone microscopes will benefit their applications in various scenarios, such as point-of-care testing, on-site diagnosis, and home health surveillance.Research highlightsWe propose a solution for ultra-low-cost smartphone microscopy. Utilizing the flexibility of 3D-printed material, we can achieve focusing accuracy of ∼ 5µm. Such a low-cost device will benefit point-of-care diagnosis and home health surveillance.

show abstract

Section: Application In the Monitoring Of Vx2 Tumor Cell Culturementioning

confidence: 80%

Towards ultra-low-cost smartphone microscopy

Zhang,

Zuo

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…This tumor can be transplanted serially from one animal to another by means of xenotransplantation and can grow in any organ or grafted tissue. A notable feature of VX2 is that it does not require genetically modified or immunocompromised subjects but can be transplanted into immunocompetent animals (Pascale et al, 2022). We observed morphological differences in VX2 tumor cells across multiple focal planes and documented the biological process of cell division in situ over a period of 1 h, demonstrating the ability of our ultra‐low‐cost smartphone microscope to have real‐time microscopic monitoring of closed cell culture processes.…”

Section: Resultsmentioning

confidence: 99%

Towards ultra‐low‐cost smartphone microscopy

Zhang,

Zuo

et al. 2024

Microscopy Res & Technique

View full text Add to dashboard Cite

The outbreak of COVID‐19 exposed the inadequacy of our technical tools for home health surveillance, and recent studies have shown the potential of smartphones as a universal optical microscopic imaging platform for such applications. However, most of them use laboratory‐grade optomechanical components and transmitted illuminations to ensure focus tuning capability and imaging quality, which keeps the cost of the equipment high. Here, we propose an ultra‐low‐cost solution for smartphone microscopy. To realize focus tunability, we designed a seesaw‐like structure capable of converting large displacements on one side into small displacements on the other (reduced to ∼9.1%), which leverages the intrinsic flexibility of 3D printing materials. We achieved a focus‐tuning accuracy of ∼5 𝜇m, which is 40 times higher than the machining accuracy of the 3D‐printed lens holder itself. For microscopic imaging, we used an off‐the‐shelf smartphone camera lens as the objective and the built‐in flashlight as the illumination. To compensate for the resulting image quality degradation, we developed a learning‐based image enhancement method. We used the CycleGAN architecture to establish the mapping from smartphone microscope images to benchtop microscope images without pairing. We verified the imaging performance on different biomedical samples. Except for the smartphone, we kept the full costs of the device under 4 USD. We think these efforts to lower the costs of smartphone microscopes will benefit their applications in various scenarios, such as point‐of‐care testing, on‐site diagnosis, and home health surveillance.Research Highlights We propose a solution for ultra‐low‐cost smartphone microscopy. Utilizing the flexibility of 3D‐printed material, we can achieve focusing accuracy of ∼5 𝜇m. Such a low‐cost device will benefit point‐of‐care diagnosis and home health surveillance.

show abstract

“…Support for this was also given by the result of experimental analysis using the rabbit VX2 CCA model. The VX2 rabbit cancer model has been applied to multiple organs, including the liver (Pascale et al 2022 ), kidney (Li et al 2019a , b ), and brain (Wang et al 2015 ). A previous study described the implantation of VX2 tumors into rabbit bile ducts (Suzuki et al 1994 ).…”

Section: Discussionmentioning

confidence: 99%

Mechanistic insights into 125I seed implantation therapy for Cholangiocarcinoma: focus on ROS-Mediated apoptosis and the role of GPX2

Luo,

Yao,

Liang

et al. 2024

J Cancer Res Clin Oncol

View full text Add to dashboard Cite

Objectives Cholangiocarcinoma (CCA) is a rare tumor with a poor prognosis and poses significant therapeutic challenges. Herein, we investigated the mechanism of efficacy of 125I seed implantation therapy in CCA, focusing on the induction of reactive oxygen species (ROS)-mediated apoptosis and the involvement of glutathione peroxidase 2 (GPX2). Materials and methods Human cholangiocarcinoma cell lines QBC939 and RBE were purchased for in vitro studies. In vivo studies were performed using a rabbit VX2 CCA model. Apoptosis and proliferation were detected by TUNEL staining and clone formation, respectively. ROS generation was detected by dihydroethidium staining. Histological evaluation was performed by hematoxylin and eosin staining. Protein expression was determined by Western blotting and immunohistochemistry. Results Our results demonstrate that 125I seeds effectively inhibited tumor growth in the rabbit VX2 tumor model and promoted the apoptosis of CCA cells in vitro in a dose-dependent manner. Molecular analyses indicate a marked increase in reactive oxygen species (ROS) levels following treatment with 125I seeds, suggesting the involvement of ROS-mediated apoptosis in the therapeutic mechanism. Furthermore, the downregulation of glutathione peroxidase 2 (GPX2) was observed, indicating its potential role in modulating ROS-mediated apoptosis in CCA. Conclusion 125I seed implantation therapy exerts therapeutic effects on CCA by inducing ROS-mediated apoptosis. The downregulation of GPX2 may contribute to enhanced ROS accumulation and apoptotic cell death. These findings provide mechanistic insights into the therapeutic potential of 125I seed implantation for CCA and highlight ROS-mediated apoptosis and GPX2 regulation as promising targets for further investigation and therapeutic intervention in this malignancy.

show abstract

Rabbit VX2 Liver Tumor Model: A Review of Clinical, Biology, Histology, and Tumor Microenvironment Characteristics

Cited by 10 publications

References 80 publications

Towards ultra-low-cost smartphone microscopy

Towards ultra-low-cost smartphone microscopy

Towards ultra‐low‐cost smartphone microscopy

Mechanistic insights into 125I seed implantation therapy for Cholangiocarcinoma: focus on ROS-Mediated apoptosis and the role of GPX2

Contact Info

Product

Resources

About