Rabies Infection: An Overview of Lyssavirus-Host Protein Interactions

Abstract

List of Abbreviations: 2-5A, 2',5'-oligoadenylate; ABLV, Australian bat lyssavirus; AKT, protein kinase B; AMPK, AMP-activated protein kinase; ATP6V1A, V type proton ATPase catalytic subunit A; CASP, caspase; Cco1, cytochrome c oxidase subunit 1; CCT, T-complex protein 1; CNS, central nervous system; Co-IP, co-immunoprecipitation; CVS, challenge virus standard; DLC1, dynein light chain 1; DUVV, Duvenhage lyssavirus; EBLV-1,2, European bat lyssaviruses type 1 and 2; eIF3h, eukaryotic translation initiation factor 3, subunit h; ESCRT, endosomal sorting complexes required for transport; FAK, focal adhesion kinase; G, glycoprotein;

Clofazimine derivatives as potent broad-spectrum antiviral agents with dual-target mechanism

“…Among all 5 proteins expressed by rabies RNA genome, L protein plays the vital role in conducting the RdRp activity in rabies virus [ 22 ]. Therefore, molecular docking analysis between compound 15f with lyssavirus L protein (PDB ID: 6EUB ) was launched by Discovery Studio 4.5, taking 1 as the control.…”

Looking at the Pathogenesis of the Rabies Lyssavirus Strain Pasteur Vaccins through a Prism of the Disorder-Based Bioinformatics

“…The amino acid sequences and the structures are the most valuable resources out of these for the purposes of studying intrinsic disorder in the virus. A set of 37 human proteins involved in interaction with the RABV proteins was assembled through the literature search, with the majority of data being retrived from [71]. Amino acid sequences and basic disorder-related features of these proteins are provided in Supplementary Figures S1, S2, S3, S4, and S5.…”

“…FuzDrop indicated that the L-protein has a low pLLPS value of 0.11860 and does not contain any DPRs. However, this protein possesses 24 In addition to be involved in interaction with the RABV L-and N-proteins and the viral ribonucleocapsids, the P-protein is known to bind to the multitude of host proteins, such as dynein light chain 1 and 2 (DYNLL1 (UniProt ID: P63167) and DYNLL2 (UniProt ID: Q96FJ2), respectively) [129], as well as host signal transducer and activator of transcription 1-alpha/beta and signal transducer and activator of transcription 2 (STAT1 (Uni-Prot ID: P42224) and STAT2 proteins (UniProt ID: P52630), respectively) [16,17,[100][101][102][103], promyelocytic leukemia (PML) protein (UniProt ID: P29590) [109], the ribosomal protein L9 (UniProt ID: Q02878) [15], STAT3 (UniProt ID: P40763), nucleolin (NCL; UniProt ID: P19338), focal adhesion kinase (FAK; UniProt ID: Q05397), Janus kinase 1 (JAK1; UniProt ID: P23458), inhibitor of nuclear factor kappa-B kinase subunit epsilon (IKKε; UniProt ID: Q14164), Beclin-1 (BECN1; UniProt ID: Q14457), tubulin alpha (TUB-α; UniProt ID: Q71U36 for tubulin alpha-1A chain), tubulin beta (TUB-β; UniProt ID: Q9H4B7 for tubulin beta-1 chain), ATP-binding cassette sub-family E member 1 (ABCE1; UniProt ID: P61221), T-complex protein 1 subunit gamma (CCTγ; UniProt ID: P49368), Hsp90 co-chaperone Cdc37 (CDC37; UniProt ID: Q16543), and heat shock protein HSP 90-alpha (Hsp90AA1; UniProt ID: P07900) [71]. Furthermore, P-protein can interact with the complex I in mitochondria leading to mitochondrial dysfunction, increased generation of reactive oxygen species (ROS), and oxidative stress [130].…”

“…Several human proteins interacting who the RABV M-protein where established. The list includes RelAp43 (which is a splicing variant of RelA (UniProt ID: Q04206) and other proteins in the NF-κB pathway [23], as well as V-type proton ATPase catalytic subunit A (ATP6V1A; UniProt ID: P38606), E3 ubiquitin-protein ligase NEDD4 (NEDD4; UniProt ID: P46934), Transcriptional coactivator YAP1 (UniProt ID; P46937), Eukaryotic translation initiation factor 3 subunit H (EIF3H, UniProt ID: O15372), JAK1 (UniProt ID: P23458), and STAT1 (UniProt ID: P63167) [71]. Note that the M-protein shares two human interactors (JAK1 and STAT1) with the P-protein [71].…”

“…Very few host proteins were shown to act as physical partners of the RABV N-protein. These include heat shock 70 kDa proteins 1A (HSPA1A, UniProt ID: P0DMV8) and 1B (HSPA1B, UniProt ID: P0DMV9), prefoldin subunit 1 (PFDN1, UniProt ID: O60925), and CCTγ (UniProt ID: P49368), with the CCTγ being a shared partner for the RABV Pand N-proteins [71].…”