RAC3 influences the chemoresistance of colon cancer cells through autophagy and apoptosis inhibition

Rubio, Miguel; Lira, María Cecilia; Rosa, Francisco; A, Sambresqui; Güemes, María Cecilia Salazar; Costas, Mónica Alejandra

doi:10.1186/s12935-017-0483-x

Cited by 26 publications

(22 citation statements)

References 51 publications

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“…The development of drug resistance among leukemia cells is the main cause of chemotherapy failure in leukemia patients, which adversely affects prognosis in patients with leukemia 20. Studies have shown that chemoresistance is associated with decreased sensitivity of tumor cells to apoptosis 2122. In this study, we discovered that PIK3CA expression is increased in Nalm-6 cells and that PIK3CA silencing enhances the sensitivity of Nalm-6 cells to chemotherapy drugs (VCR and DNR) by suppressing the phosphorylation of Akt.…”

Section: Discussionmentioning

confidence: 72%

Silencing thePIK3CAGene Enhances the Sensitivity of Childhood Leukemia Cells to Chemotherapy Drugs by Suppressing the Phosphorylation of Akt

Liang

Xin

et al. 2019

Yonsei Med J

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PurposeThis study aimed to investigate the effects of PIK3CA on the sensitivity of acute B lymphocytic leukemia cells (Nalm-6 cells) to chemotherapy drugs.Materials and MethodsChildren's normal B lymphocytes and Nalm-6 cells were cultured. Nalm-6 cells were transfected with PIK3CA siRNA (siPIK3CA group) or its negative control (PIK3CA-Control group). Normal Nalm-6 cells were named Mock group. Nalm-6 cells transfected by PIK3CA siRNA were treated with Akt inhibitor (siPIK3CA+Akti-1/2 group). mRNA and protein expression was detected by qRT-PCR and Western blot. Proliferation and sensitivity to chemotherapeutic drugs was detected by MTT assay. Cell cycle and apoptosis was explored by low cytometry. Transwell assay was performed to test invasion.ResultsPIK3CA mRNA (p=0.008) and protein (p=0.006) expression was higher in Nalm-6 cells than that in normal B lymphocytes. Compared with the Mock group and PIK3CA-Control group, Nalm-6 cells of the siPIK3CA group had lower OD495 values (all p<0.05) and invasion cell numbers (p=0.03 and p=0.025), as well as a higher proportion of G0/G1 phase cells (p=0.020 and p=0.022), percentage of apoptosis (p=0.016 and p=0.022), and inhibition rate (all p<0.05). pAkt expression in the siPIK3CA group (p=0.026 and p=0.031) and siPIK3CA+Akti-1/2 group (p=0.019 and p=0.023) was lower than that in the Mock group.ConclusionPIK3CA silencing inhibited Nalm-6 cell proliferation and invasion, and promoted their apoptosis and sensitivity to chemotherapeutic drugs, potentially through regulation of the PI3K/AKT signaling pathway.

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Section: Discussionmentioning

confidence: 72%

Silencing thePIK3CAGene Enhances the Sensitivity of Childhood Leukemia Cells to Chemotherapy Drugs by Suppressing the Phosphorylation of Akt

Liang

Xin

et al. 2019

Yonsei Med J

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“…The results indicated the role of RAC3 on endothelial dysfunction by down-regulating autophagy [ 39 ]. Rubio et al demonstrate that the expression level of RAC3 was negatively associated with autophagy, influencing chemoresistance of colorectal cancer [ 40 ]. In a word, RAC3 was verified to have a capacity for down-regulating autophagy.…”

Section: Discussionmentioning

confidence: 99%

FAM201A, a long noncoding RNA potentially associated with atrial fibrillation identified by ceRNA network analyses and WGCNA

Chen

Dan

et al. 2022

BMC Med Genomics

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Background Being the most common arrhythmia in clinic, atrial fibrillation (AF) causes various comorbidities to patients such as heart failure and stroke. LncRNAs were reported involved in pathogenesis of AF, yet, little is known about AF-associated lncRNAs. The present study aims to explore lncRNAs associated with AF susceptibility based on competing endogenous RNA (ceRNA) network analysis and weighted gene co-expression network analysis (WGCNA). Methods GSE41177 and GSE79768 datasets were obtained from the Gene Expression Omnibus (GEO) database. Competing endogenous RNA (ceRNA) network analysis was performed using GSE41177. Differentially expressed lncRNAs (DElncRNAs), mRNAs (DEmRNAs) between AF patients and patients with sinus rhythm (SR) were identified from GSE41177 using R software. Then, the ceRNA network was constructed based on DElncRNAs, the predicted target miRNAs and DEmRNAs. Weighted gene co-expression network analysis (WGCNA) was performed using GSE79768 to validate the AF-related lncRNAs identified from GSE41177. LncRNA modules and crucial lncRNAs relevant to AF and were identified. Results In summary, 18 DElncRNAs and 350 DEmRNAs were found between AF patients and SR patients. A total of 5 lncRNAs, 10 miRNAs, and 21 mRNAs were contained in the final ceRNA network. Taking into consideration both the ceRNA theory and inference scores from the comparative toxicogenomics database (CTD) database, the ceRNA axis FAM201A-miR-33a-3p-RAC3 was identified as mostly relevant to AF susceptibility. FAM201A (Gene significance, GS = − 0.62; Module membership, MM = 0.75) was also proved in the blue module, which was identified most highly relevant with AF by WGCNA. Conclusions These results demonstrated that decreased expression of FAM201A might be associated with susceptibility of AF. Working as the ceRNA to regulate RAC3 might be one function of FAM201A in AF susceptibility, which requires further exploration in future research.

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“…The results indicated the role of RAC3 on endothelial dysfunction by down-regulating autophagy [38]. Rubio et al demonstrate that the expression level of RAC3 was negatively associated with autophagy, in uencing chemoresistance of colorectal cancer [39]. In a word, RAC3 was veri ed to have a capacity for down-regulating autophagy.…”

Section: Discussionmentioning

confidence: 99%

FAM201A, a Long Noncoding RNA Potentially Associated With Atrial Fibrillation Identified by ceRNA Network Analyses and WGCNA

Chen

Dan

et al. 2021

Preprint

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Background: Atrial fibrillation (AF) is the most common cardiac arrhythmia that contributes to various complications. However, little is known about lncRNAs associated with AF susceptibility. In the present study, we aim to identify lncRNAs involved in pathogenesis of AF based on competing endogenous RNA (ceRNA) network analyses and weighted gene co-expression network analysis (WGCNA).Methods: Two lncRNA and mRNA microarray datasets GSE41177 and GSE79768 were retrieved from the Gene Expression Omnibus (GEO) database. Differentially expressed lncRNAs (DElncRNAs), mRNAs (DEmRNAs) between AF patients and patients with sinus rhythm (SR) were identified from dataset GSE41177. Then, those DElncRNAs associated target miRNAs were predicted. The ceRNA network was constructed based on DElncRNAs, predicted miRNAs and DEmRNAs. To validate the role of AF-related lncRNAs, all lncRNAs form dataset GSE79768 were selected to perform WGCNA. LncRNA modules relevant to AF were identified. Crucial lncRNAs in the module that was most relevant to AF were screened according to the criteria of | Gene significance (GS)| > 0.6 and |Module membership (MM)| > 0.5. Results: A total of 18 DElncRNAs and 350 DEmRNAs were identified between AF patients and SR patients. The final ceRNA network contained 5 lncRNAs, 10 miRNAs, and 21 mRNAs. According to the ceRNA theory, combined with the comparative toxicogenomics database (CTD) database, the ceRNA axis FAM201A-miR-33a-3p-RAC3 was considered associated with AF susceptibility. By WGCNA, the blue module was detected most highly relevant with AF. The lncRNA FAM201A was proved in the blue module and highly related to AF. Conclusions: These results demonstrated that FAM201A might have great potential for susceptibility of AF based on ceRNA network analyses and WGCNA. FAM201A may function, at least partly, as ceRNA to regulate RAC3 in AF susceptibility.

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RAC3 influences the chemoresistance of colon cancer cells through autophagy and apoptosis inhibition

Cited by 26 publications

References 51 publications

Silencing thePIK3CAGene Enhances the Sensitivity of Childhood Leukemia Cells to Chemotherapy Drugs by Suppressing the Phosphorylation of Akt

Silencing thePIK3CAGene Enhances the Sensitivity of Childhood Leukemia Cells to Chemotherapy Drugs by Suppressing the Phosphorylation of Akt

FAM201A, a long noncoding RNA potentially associated with atrial fibrillation identified by ceRNA network analyses and WGCNA

FAM201A, a Long Noncoding RNA Potentially Associated With Atrial Fibrillation Identified by ceRNA Network Analyses and WGCNA

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