Racemization of Lens Crystalline Constituents in UV-lnduced Cataract Evaluated by Chiral GC/MS Spectroscopy

Abstract

The racemization of amino acids is hypothesized to caust cataract by disrupting the crystalline’s tertiary structure which, in turn, alters the optical characteristics of the lens. Tc better understand the role of these modifications in cataracto·genesis, the changes in stereoisomer ratio of amino acids fron lens crystalline by UV-B-induced cataract in animal models were studied using chiral separation gas chromatography-single quadrupole mass spectroscopy. The anticataract actior of a compound, {[l-(phenylmethyl)-lH-indazol-3-yl]oxy}acetk acid lysinate, was also evaluated by this method.