Objective: Our aim was to assess students’ preparedness for the smoking cessation role after a teaching module on tobacco cessation and to assess the relationship between smoking status and preparedness. Methods: Pharmacy students attended a 4-hour module on the role of health professionals in smoking cessation based on the Rx for Change training program. Two years after the first introduction of the module, a questionnaire on preparedness and smoking status was filled in by 64 pharmacy students who had attended the module and 243 students from other health sciences that had not been exposed to the module. Results: Preparedness scores for the smoking cessation role for pharmacy students were significantly higher (mean = 19.5, n = 63) for the nonsmoking pharmacy students compared to other nonsmoking students in medicine (mean = 16.2, n = 149), dentistry (mean = 16.1, n = 40) and physical therapy (mean = 14.9, n = 16) (p = 0.009). The smoking prevalence was 3% among all females and 31% among all males. Smoking students were significantly less likely (p = 0.031) to agree that it is their professional responsibility to help patients quit smoking. Conclusion: Significant and long-lasting differences in students’ perception of preparedness for the smoking cessation role based on exposure to a short teaching module were demonstrated. Furthermore, our results support previous findings that smoking compromises attitude towards helping smokers to quit.