Rad54, a Swi2/Snf2-like Recombinational Repair Protein, Disassembles Rad51:dsDNA Filaments

Solinger, Jachen A.; Kiianitsa, Kostantin; Heyer, Wolf Dietrich

doi:10.1016/s1097-2765(02)00743-8

Cited by 245 publications

(272 citation statements)

References 42 publications

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“…Both proteins form the active nucleoprotein filaments on ssDNA but also bind dsDNA with an affinity that is only slightly less than their ssDNA binding (see Morrical 2014;Brown and Bishop 2015). Thus, Rad51 and Dmc1 form dead-end complexes on duplex DNA, unless they are constantly dissociated by the motor proteins Rad54 and Rdh54/Tid1 (Solinger et al 2002;Holzen et al 2006;Shah et al 2010). Although such deadend complexes are not direct intermediates in the HR pathway, they illustrate the need for reversibility of DNA complexes formed by HR proteins.…”

Section: Reversibility At the Rad51 Nucleoprotein Filament Stage: Resmentioning

confidence: 99%

Regulation of Recombination and Genomic Maintenance

Heyer

2015

Cold Spring Harb Perspect Biol

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104

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Recombination is a central process to stably maintain and transmit a genome through somatic cell divisions and to new generations. Hence, recombination needs to be coordinated with other events occurring on the DNA template, such as DNA replication, transcription, and the specialized chromosomal functions at centromeres and telomeres. Moreover, regulation with respect to the cell-cycle stage is required as much as spatiotemporal coordination within the nuclear volume. These regulatory mechanisms impinge on the DNA substrate through modifications of the chromatin and directly on recombination proteins through a myriad of posttranslational modifications (PTMs) and additional mechanisms. Although recombination is primarily appreciated to maintain genomic stability, the process also contributes to gross chromosomal arrangements and copy-number changes. Hence, the recombination process itself requires quality control to ensure high fidelity and avoid genomic instability. Evidently, recombination and its regulatory processes have significant impact on human disease, specifically cancer and, possibly, neurodegenerative diseases.

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Section: Reversibility At the Rad51 Nucleoprotein Filament Stage: Resmentioning

confidence: 99%

Regulation of Recombination and Genomic Maintenance

Heyer

2015

Cold Spring Harb Perspect Biol

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104

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“…Such structure arises after recombination-mediated invasion of a single-stranded end into a homologous duplex, as can be envisioned at arrested replication forks. Rad54 is thought to be crucial for facilitating formation and movement of the D-loop (Solinger et al, 2002;Mazin et al, 2003;Li et al, 2007). Therefore, given the incomplete epistatic relationship between mph1 and rad54, it seems reasonable to place Mph1 at the Rad51-mediated strand invasion process, with a function distinct from that of Rad54.…”

Section: Mph1 Probably Acts At a Stage After Initiation Of Recombinationmentioning

confidence: 99%

Genetic evidence for a role of Saccharomyces cerevisiae Mph1 in recombinational DNA repair under replicative stress

Panico

Ede

Schildmann

et al. 2009

Yeast

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In yeast as in human, DNA helicases play critical roles in assisting replication fork progression. The Saccharomyces cerevisiae MPH1 gene, homologue of human FANCM, has been involved in homologous recombination and DNA repair. We describe a synthetic growth defect of an mph1 deletion if combined with an srs2 deletion that can result -depending on the genetic background -in synthetic lethality. The lethality is suppressed by mutations in homologous recombination (rad51, rad52, rad55, rad57 ) and in the DNA damage checkpoint (rad9, rad24, rad17 ). Importantly, rad54 and mph1, epistatic for damage sensitivity, are subadditive for spontaneous mutator phenotype. Therefore, Mph1 could be placed at the Rad51-mediated strand invasion process, with a function distinct from Rad54. Moreover, siz1 mutation is viable with mph1 and additive for DNA damage sensitivity. mph1 srs2 double mutants, isolated in a background where they are viable, are synergistically sensitive to DNA damage. Moderate overexpression of SGS1 partially suppresses this sensitivity. Finally, we observe an epistatic relationship in terms of sensitivity to camptothecin of mms4 or mus81 to mph1. Overall, our results support a role of Mph1 in assisting replication progression. We propose two models for the resumption of DNA synthesis under replicative stress where Mph1 is placed at the sister chromatid interaction step.

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“…115 These results are consistent with the ability of Rad54 to displace Rad51 from DNA in an ATP-dependent reaction. 116,117 In contrast to previous reports, Shina and Peterson 115 suggest that the chromatin remodeling activity of SWI/SNF does not generally enhance homology search in chromatin donors. The role of SWI/SNF in HR repair may be restricted to situations in which the donor locus is embedded in a highly condensed, heterochromatic chromatin structure.…”

confidence: 66%

Chromatin dynamics coupled to DNA repair

Huertas

Sendra²,

Muñoz³

2009

Epigenetics

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In order to protect and preserve the integrity of the genome, eukaryotic cells have developed accurate DNA repair pathways involving a coordinated network of DNA repair and epigenetic factors. The DNA damage response has to proceed in the context of chromatin, a packaged and compact structure that is flexible enough to regulate the accession of the DNA repair machinery to DNA-damaged sites. Chromatin modifications and ATP-remodeling activities are both necessary to ensure efficient DNA repair. Here we review the current progress of research into the importance of chromatin modifications and the ATP-remodeling complex to the DNA damage response, with respect to the sensing and signaling of DNA lesions, DNA repair and the processes that restore chromatin structure.

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Rad54, a Swi2/Snf2-like Recombinational Repair Protein, Disassembles Rad51:dsDNA Filaments

Cited by 245 publications

References 42 publications

Regulation of Recombination and Genomic Maintenance

Regulation of Recombination and Genomic Maintenance

Genetic evidence for a role of Saccharomyces cerevisiae Mph1 in recombinational DNA repair under replicative stress

Chromatin dynamics coupled to DNA repair

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