Radial extracorporeal shock wave reduces myogenic contracture and muscle atrophy via inhibiting NF-κB/HIF-1α signaling pathway in rabbit

Wang, Feng; Li, Wen; Zhou, Yun; Huang, Peng; Zhang, Quan Bing

doi:10.1080/03008207.2021.1920934

Cited by 12 publications

(17 citation statements)

References 34 publications

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“…In the histologic assessment of the present study, the myo ber cross-sectional area, intermuscular collagen deposition, and extent of hyperplasia in the anterior joint capsule supported the biological ndings. The myo ber cross-sectional area was signi cantly smaller in the immobilization-3d group than the control group, not in group immobilization-3w, after 3 days after 2 weeks of immobilization, as the decreased skeletal muscle mass caused by an imbalance in protein metabolism is characterized by a signi cantly smaller muscle ber area [25,26,27]. The amount of intermuscular collagen deposition was signi cantly greater in the immobilization-1w group than the control group, but did not signi cantly differ between the immobilization-2w and − 3w groups.…”

Section: Discussionmentioning

confidence: 97%

A Novel External Immobilization Method to Establish a Rat Model of Knee Extension Contracture

Zhou

Wang

et al. 2022

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Background: The study aimed to elucidate the formation process and therapeutic strategies of knee extension contracture, we developed a novel rat model using an aluminum external fixator.Methods: Sixty male SD rats with mature bones were divided into the control group (n=6) and groups that had the left knee immobilized with an aluminum external fixator for 1, 2, and 3 days, and 1, 2, 3, 4, 6, and 8 weeks (n=6 in each group). The passive extension range of motion, histology, and expression of fibrosis-related proteins were compared between the control group and the immobilization groups.Results: Myogenic contracture progressed very quickly during the initial 2 weeks of immobilization; after 2 weeks, the contracture gradually changed from myogenic to arthrogenic. The arthrogenic contracture progressed slowly during the first week, rapidly progressed until the third week, and then showed a steady progression. Histological analysis confirmed that the anterior joint capsule of the extended fixed knee became increasingly thicker over time. Correspondingly, the level of TGFβ-1 in the anterior joint capsule also increased with the immobilization time. Over time, the cross-sectional area of muscle fibers gradually decreased, while the amount of intermuscular collagen and TGFβ-1 increased. Unexpectedly, the amount of intermuscular collagen and TGFβ-1 decreased during the late stage of immobilization (6–8 weeks).Conclusion: Myogenic contracture is stabilized after 2 weeks of immobilization, while arthrogenic contracture is stabilized after 3 weeks of immobilization. This novel rat model may be a useful tool to study the etiology of joint contracture and establish new therapeutic approaches.

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Section: Discussionmentioning

confidence: 97%

A Novel External Immobilization Method to Establish a Rat Model of Knee Extension Contracture

Zhou

Wang

et al. 2022

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“…It is also a safe and effective non-invasive treatment for many fibrotic diseases such as myogenic contracture, pathological scars, epidural fibrosis, etc. [13][14][15][16][17][18][19][20]. In this study, extension knee joint contracture in rats was treated in different ways, to explore whether the treatment effect of SPS combined with ESWT is better than single treatment, and to guide clinical practice.…”

Section: Discussionmentioning

confidence: 99%

“…Extracorporeal shock wave therapy (ESWT) is widely used in musculoskeletal system diseases [ 13 , 14 ]. The mechanical stimulation produced by ESWT has biochemical effects on some cells and affects the cell cycle and metabolic pathways, which can regulate the migration, proliferation, differentiation and apoptosis of fibroblasts, endothelial cells, osteoblasts, and tenocytes [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, ESWT can significantly and effectively reduce the fibrosis in the treatment of epidural fibrosis [ 18 ] and capsular fibrosis after insertion of silicone implants [ 19 ]. Huang et al [ 13 ] found that radial ESWT combined with ultrashort wave therapy could inhibit the high expression of TGF-β1 and hypoxia-inducible factor 1 alpha (HIF-1α), and radial ESWT could inhibit the NF-κB/HIF-1α signaling pathway [ 14 ], which could reduce the myogenic contracture after long-term immobilization. A study reported that the adhesion between the lateral femoral condyle and the joint capsule was significantly lower than that of the control group after ESWT during the repair of the knee in rabbits [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Possible mechanism of static progressive stretching combined with extracorporeal shock wave therapy in reducing knee joint contracture in rats based on MAPK/ERK pathway

Zhang

Zhou

et al. 2022

Bosn J of Basic Med Sci

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The study aimed to observe the therapeutic effect of static progressive stretching (SPS) combined with extracorporeal shock wave therapy (ESWT) on extension knee joint contracture in rats and the effect on the MAPK/ERK pathway in the development of joint capsule fibrosis. Thirty-six Sprague Dawley rats were randomly divided into blank control group, immobilization model group, natural recovery group, ESWT intervention group, SPS intervention group, and SPS combined with ESWT intervention group. The left knee joints of the rats, except for the control group, were fixed with an external fixation brace for four weeks at full extension to form joint contractures. The therapeutic effect of each intervention was assessed by evaluating total and arthrogenic contracture, the number of total cells and collagen deposition in the anterior joint capsule, the protein levels of TGF-β1, FGF-2, and ERK2 in the anterior joint capsule, the mean optical density of upstream RAS and downstream ERK2 positive expression in the MAPK/ERK pathway. SPS in combination with ESWT was more effective in relieving joint contracture, improving the histopathological changes in the anterior joint capsule, and suppressing the high expression of target proteins and the overactivated MAPK/ERK pathway. The overactivated MAPK/ERK pathway was involved in the formation of extension knee joint contracture in rats. SPS in combination with ESWT was effective in relieving joint contracture and fibrosis of joint capsule. Moreover, the inhibition of the overactivated MAPK/ERK pathway may be the potential molecular mechanism for its therapeutic effect.

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“…This phenomenon indicates that the catabolism of muscle protein in skeletal muscle tissue was enhanced. Our previous studies suggest that the occurrence of disuse muscular atrophy is related to the happening of joint contracture [5,9]. The decrease in muscle wet weights is one of the most direct re ections of muscle atrophy [10].…”

Section: Introductionmentioning

confidence: 99%

Electrical Stimulation Attenuates Disuse Muscular Atrophy by Modulating Endoplasmic Reticulum Stress-induced Parkin-dependent Mitophagy

Zhang

Liu

Fang

et al. 2022

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Background: The aim of this study was to investigate the therapeutic effect of electrical stimulation on disuse muscular atrophy in a rabbit model of knee joint contracture and explore the role of endoplasmic reticulum stress-induced Parkin-dependent mitophagy in this process.Methods: Two sub-experiments were carried out successively in our study. In the first sub-experiment, 24 rabbits were divided into four groups on average based on the immobilization time: Ctrl 1, I-2, I-4, and I-6 groups. In the second sub-experiment, 24 rabbits were also divided into four groups on average in accordance with the process mode: Ctrl2, ES, NR, and EST groups. To test the time-dependent changes of the rectus femoris muscles after immobilization in rabbits, and to evaluate the effect of electrical stimulation on the atrophic rectus femoris muscles, the wet weights of rectus femoris muscles were assessed in this study, along with the protein levels of atrogin-1, p-PERK, Parkin and COXIV.Results: The wet weights of rectus femoris muscles, the protein levels of atrogin-1, p-PERK and Parkin increased after immobilization. It was also revealed that the protein levels of COXIV decreased after immobilization. Electrical stimulation was effective against muscle atrophy, the elevated expression of atrogin-1, p-PERK, Parkin, and the decreased expression of COXIV.Conclusions: Immobilization of unilateral lower limb could induce rectus femoris muscle atrophy, endoplasmic reticulum stress and Parkin mediated mitophagy. Endoplasmic reticulum stress-induced Parkin-dependent mitophagy may be one of the mechanisms by which electrical stimulation can play a significant role.

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Radial extracorporeal shock wave reduces myogenic contracture and muscle atrophy via inhibiting NF-κB/HIF-1α signaling pathway in rabbit

Cited by 12 publications

References 34 publications

A Novel External Immobilization Method to Establish a Rat Model of Knee Extension Contracture

A Novel External Immobilization Method to Establish a Rat Model of Knee Extension Contracture

Possible mechanism of static progressive stretching combined with extracorporeal shock wave therapy in reducing knee joint contracture in rats based on MAPK/ERK pathway

Electrical Stimulation Attenuates Disuse Muscular Atrophy by Modulating Endoplasmic Reticulum Stress-induced Parkin-dependent Mitophagy

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