Radial profile detection of multiple spherical particles in contact with interacting surfaces

Waschke, Johannes; Pompe, Tilo; Rettke, David; Schmidt, Stephan; Hlawitschka, Mario

doi:10.1371/journal.pone.0214815

Cited by 8 publications

(7 citation statements)

References 23 publications

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“…Obtained images of radial profiles resulting from the respective adhering or non-adhering SCPs were analyzed by a home-built software based on pattern matching. 32 Contact areas, particle radii as well as adhesion energies were calculated by the software.…”

Section: Methodsmentioning

confidence: 99%

Microfluidics-assisted synthesis and functionalization of monodisperse colloidal hydrogel particles for optomechanical biosensors

et al. 2022

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Section: Methodsmentioning

confidence: 99%

Microfluidics-assisted synthesis and functionalization of monodisperse colloidal hydrogel particles for optomechanical biosensors

et al. 2022

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“…Images with RICM patterns were read out using self‐written image analysis software, and contact areas and particle profiles were evaluated using home written software. [ 57 ] After adding the SCPs to the ConA‐coated glass slides, the temperature‐dependent adhesion measurements were conducted in three cycles: 1) at 20 °C after 30 min equilibration, 2) at 45 °C after 60 min equilibration, and 3) 20 °C after 60 min equilibration.…”

Section: Methodsmentioning

confidence: 99%

Quantifying Thermoswitchable Carbohydrate‐Mediated Interactions via Soft Colloidal Probe Adhesion Studies

Strzelczyk

Paul

Schmidt

2020

Macromolecular Bioscience

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affinity of linked biomolecules, e.g., by varying their accessibility to control their specific binding. Thermoresponsive polymers with a lower critical solution temperature (LCST) between 30 and 40 °C are most frequently used for such applications, where poly(N-isopropyl acrylamide) (PNIPAM), poly(N-vinyl caprolactam) or poly(oligoethylene glycols) are well-known examples. [10] As bioligands conjugated to such LCST polymers, carbohydrates have recently gained attention since they dominate biomolecular interactions on the cellular level and drive numerous physiological processes in the healthy or diseased state. [11] For example, lectins, a class of carbohydrate binding proteins, mediate cell adhesion, communication, fertilization, or pathogen invasion. [12,13] To target carbohydrate binding pathogens or lectins directly, responsive carbohydrate ligand presenting polymers are being employed in microgels, [14-16] on nano particle surfaces, [17-19] 2D-surface coatings, [4] and linear or branched polymers. [20-23] Although many studies could show a temperature controllable affinity shift of thermoresponsive glycopolymers, the cause for this behavior and the molecular details are not well understood. For example, when increasing the temperature above the LCST, some studies found that the affinity increased, [16,17,24] whereas other studies obtained decreasing carbohydrate binding affinities. [4,20,21] The factors that may increase the binding affinities upon temperature increase are: 1) an increase of carbohydrate subunit density due to an increase of statistical rebinding or subsite binding. [25-27] 2) An increase of carbohydrate surface density due to the formation of a compact polymer globule where the hydrophilic carbohydrates enrich at the surface. [28] 3) A smoother surface upon polymer collapse leading to a reduced steric repulsion. [29,30] On the other hand, the collapse of the thermoresponsive polymer above the LCST can lead to a reduced accessibility of the carbohydrate units, e.g., due to the more compact polymer globules or aggregate formation. [21] These potentially negating mechanisms make it hard to predict the change in glycopolymer affinity upon the temperature induced coil-to-globule transition. In addition, the use of these materials is often motivated by being able to remotely "switch" the ligand-receptor interaction on and off, implying reversible ligand-receptor complex formation and dissociation. However, such reversible binding of LCST polymers was rarely shown and typically limited to Thermosensitive polymers enable externally controllable biomolecular interactions but hysteresis effects hamper the reversibility and repeated use of these materials. To quantify the temperature-dependent interactions and hysteresis effects, an optical adhesion assay based on poly(ethylene glycol) microgels (soft colloidal probes, SCPs) with mannose binding concanavalin A surfaces is used. A series of thermoresponsive glycopolymers is synthesized varying the carbohydrate type, their density, and linker type, and...

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“…Our adhesion studies with soft hydrogel‐based probes have shown that material parameters such as the scaffold rigidity have a strong effect on carbohydrate interactions. The SCPs are ideal for fast, high throughput optical readout but can also serve as a biomimetic colloidal probe in AFM to directly study adhesive properties on cells . This will be particularly useful to investigate the molecular mechanisms underlying the cell‐instructive properties of specifically interacting carbohydrate functionalized scaffolds …”

Section: Switchable Carbohydrate Presenting Scaffoldsmentioning

confidence: 99%

“…The adhesion leads to mechanical deformation of the probe, which can be quantified by measuring its contact radius a and overall shape by RICM. [18][19][20] With the previously determined elastic modulus of the probes, the adhesion energies can be then quantified by the JKR (Johnson-Kendall-Roberts) approach. [14] showed an identical ranking in terms of ConA binding energy.…”

Section: Effect Of Ligand Presentation On the Affinity Of Carbohydratmentioning

confidence: 99%

“…The SCP adhesion assay: Ligand functionalized hydrogel‐based SCPs adhere to a receptor functionalized glass slide. The adhesion leads to mechanical deformation of the probe, which can be quantified by measuring its contact radius a and overall shape by RICM . With the previously determined elastic modulus of the probes, the adhesion energies can be then quantified by the JKR (Johnson–Kendall–Roberts) approach …”

Section: Introductionmentioning

confidence: 99%

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Interactive Polymer Gels as Biomimetic Sensors for Carbohydrate Interactions and Capture–Release Devices for Pathogens

Schmidt

Paul

Strzelczyk

2019

Macro Chemistry & Physics

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Adhesive processes mediated by carbohydrate‐decorated interfaces play a crucial role in many biological processes such as cell development or pathogen invasion. The involved carbohydrate scaffolds are soft and present multiple subsites forming complex and dynamic bonds to carbohydrate binding proteins. New tools and data are needed to understand how ligand presentation and mechanical properties drive these binding processes. This article highlights recent developments in the area of adhesion assays with a focus on soft biomimetic carbohydrate scaffolds as probes of adhesion forces. Key findings state that carbohydrate functionalized polymer networks largely show additive multivalency (statistical effects) and that the overall interaction forces are strongly affected by the stiffness of the network. These results indicate that phase transitions of carbohydrate bearing polymer gels may enable tunable affinity toward carbohydrate binding proteins. As an example, polymer networks undergoing large changes in mechanical rigidity, density, and spacing of carbohydrate ligands upon temperature stimulus are shown to bind or release carbohydrate binding bacteria such as Escherichia coli. The presented adhesion assays and the developed responsive systems can provide new insights into the mechanism through which carbohydrates mediate adhesion processes and establish new avenues toward scaffolds for the capture or release of cells or pathogens.

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Radial profile detection of multiple spherical particles in contact with interacting surfaces

Cited by 8 publications

References 23 publications

Microfluidics-assisted synthesis and functionalization of monodisperse colloidal hydrogel particles for optomechanical biosensors

Microfluidics-assisted synthesis and functionalization of monodisperse colloidal hydrogel particles for optomechanical biosensors

Quantifying Thermoswitchable Carbohydrate‐Mediated Interactions via Soft Colloidal Probe Adhesion Studies

Interactive Polymer Gels as Biomimetic Sensors for Carbohydrate Interactions and Capture–Release Devices for Pathogens

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