Radial segmentation of leukocyte nuclei in some rheumatic disorders

Core, P.; Muti, S.; Cervini, C

doi:10.1007/bf00290203

Cited by 2 publications

(3 citation statements)

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“…The term radial segmentation (RS) is applied to a characteristic nuclear deformation, occurring in vivo in many neoplastic and leukemic cells, but often also as an in vitro artifact in different conditions [ 28 ]. Furthermore, the RS phenomenon is more frequent in infectious, inflammatory, and necrotizing diseases than in healthy subjects [ 29 ]. The normal segmentation of the nucleus is quite different from RS due to the pathologic or toxic environment, for example, storage in a culture medium with in EDTA sodium oxalate.…”

Section: Resultsmentioning

confidence: 99%

“…For the evaluation of cytoskeletal lymphocytes, the indirect test of RS formation was used. Radial segmentation is a fascinating phenomenon, yet to be fully explained, that involves the leucocyte nuclei of various diseases [ 29 ]. RS of nuclei may provide a convenient and reliable screening test for metaphase-blocking activities of new substances [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

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Investigation of the immune effects of Scutellaria baicalensis on blood leukocytes and selected organs of the chicken’s lymphatic system

Króliczewska

Graczyk

Króliczewski

et al. 2017

J Animal Sci Biotechnol

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BackgroundThe health of chickens and the welfare of poultry industry are central to the efforts of addressing global food security. Therefore, it is essential to study chicken immunology to maintain and improve its health and to find novel and sustainable solutions. This paper presents a study on investigation of the effect of Scutellaria baicalensis root (SBR) on the immune response of broiler chicken, especially on lymphocytes and heterophils reactivity, regarding their contribution to the development of immunity of the chickens.MethodsThe 121-day-old Hubbard Hi-Y male broiler hybrids were randomly assigned to four treatment groups, three SBR supplemented groups (0.5, 1.0, and 1.5% of SBR) and one control group. Each treatment was replicated five times with six birds per replicate pen in a battery brooder. Blood was collected after 3rd and 6th wk of the experiment, and hemoglobin and hematocrit values were determined, as well as total leukocyte count and differential count were performed. Nitroblue tetrazolium test and phagocytosis assay as nonspecific immune parameters and humoral immune responses to the antigenic challenge by sheep red blood cells were performed. Moreover, the ability of peripheral blood lymphocytes to form radial segmentation (RS) of their nuclei was analyzed. Body weight and relative weight of spleen, liver, and bursa of Fabricius were recorded.ResultsResults showed that mean heterophile/lymphocyte ratio increased in the SBR groups compared to the control group and the blood of the chickens showed lymphocytic depletion. The results also demonstrated that the relative weight of bursa of Fabricius and spleen in groups fed with SBR significantly decreased compared to the control group. This study also showed that the addition of SBR significantly inhibited the formation of RS of nuclei compared to some cytotoxic substances.ConclusionWe found that SBR supplementation should be carefully evaluated when given to poultry. The excess intake of SBR supplementation may cause immunologic inhibition and may negatively affect the development of immune organs. SBR has inhibited the formation of radial segmentation nuclei showing antimetastatic properties and also the phagocytosis of chicken heterophils.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Investigation of the immune effects of Scutellaria baicalensis on blood leukocytes and selected organs of the chicken’s lymphatic system

Króliczewska

Graczyk

Króliczewski

et al. 2017

J Animal Sci Biotechnol

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“…2 Botryoid nuclei have been described in patients with hyperthermia due to cocaine and methamphetamine use, 3 malignant hyperthermia, neuroleptic malignant syndrome, 4 and autoimmune disorders such as rheumatoid arthritis, psoriatic arthritis, and systemic sclerosis. 5 In comparison, the multilobed nuclei in cobalamin and folate deficiency appear disorganized.…”

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confidence: 96%

Hypersegmented Neutrophils in an Adolescent Male With Heatstroke

Im¹,

Chan

2015

Journal of Pediatric Hematology/Oncology

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A 14-year-old male presented to the hospital after syn cope during football practice on a hot summer day. On examination, temperature was 40.41C (104.71F) and heart rate was 180 beats per minute. He was unresponsive, intubated, and cooled in the pediatric intensive care unit. Laboratory values revealed acute kidney injury and ischemic hepatitis. Complete blood count showed a white blood cell count of 17 Â 10 9 /L, hemoglobin of 14.8 g/L, platelet count of 395Â10 9 /L, and mean corpuscular volume of 83.8 fL (normal, 81.4 to 91.9 fL). Peripheral blood smear showed hypersegmented neutrophils with "botryoid" nuclei (Supplemental Fig. 1 Supplemental Digital Content 1, http://links.lww.com/JPHO/A98).Hypersegmented neutrophils are classically seen with folate (vitamin B 9 ) orcobalamin (vitamin B 12 ) deficiency. These morphologic changes of the neutrophil nucleus occur due to impaired DNA synthesis from inadequate substrate or impaired replication from a toxin or medication effect. Arrest of nuclear maturation, impaired cell division, and unbalanced cell growth results in characteristic large cells with immature nuclei with relative cytoplasmic maturity. Red blood cell macrocytosis often accompanies hypersegmented neutrophils and can be seen in hypothyroidism, alcohol abuse, uremia, and myelodysplastic syndromes.Hypersegmented neutrophils without red blood cell macrocytosis, as in our patient, has been described in patients with hyperthermia, uremia, and concurrent megaloblastic and microcytic anemia from combined folate and/ or cobalamin deficiency along with iron deficiency or thalassemia. As the finding of hypersegmented neutrophils preceeds macrocytosis, neutrophil hypersegmentation without macrocytosismay represent early cobalamin and folate deficiency. 1 The term "botryoid" refers to nuclei that appear like a cluster of grapes around a stem. 2 Botryoid nuclei have been described in patients with hyperthermia due to cocaine and methamphetamine use, 3 malignant hyperthermia, neuroleptic malignant syndrome, 4 and autoimmune disorders such as rheumatoid arthritis, psoriatic arthritis, and systemic sclerosis. 5 In comparison, the multilobed nuclei in cobalamin and folate deficiency appear disorganized.The pathogenesis of botryoid nuclei in hyperthermia has not been clearly elucidated. Hyperthermia may activate the intrinsic signaling pathway that initiates apoptosis since pyknosis, nuclear condensation in the setting of irreversible cell death, is seen in neutrophils in response to hyperthermia. 6 In addition, microtubular decomposition may play a role. 7 In vitro experiments have shown that radially segmented neutrophils are induced by applying heat. 8 Botryoid changes in >50% of neutrophils on a peripheral blood smear may be sufficient to diagnose heatstroke. 2 Fewer than 50% botryoid neutrophils is suggestive of heatstroke. Clinicians should be aware that hyperthermia can cause hypersegmented neutrophils so as to avoid unnecessary evaluations for other etiologies.Our patient's mental status and renal function impro...

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Radial segmentation of leukocyte nuclei in some rheumatic disorders

Cited by 2 publications

References 5 publications

Investigation of the immune effects of Scutellaria baicalensis on blood leukocytes and selected organs of the chicken’s lymphatic system

Investigation of the immune effects of Scutellaria baicalensis on blood leukocytes and selected organs of the chicken’s lymphatic system

Hypersegmented Neutrophils in an Adolescent Male With Heatstroke

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